RGS16 (NM_002928) Human Tagged ORF Clone

CAT#: RC202430

RGS16 (Myc-DDK-tagged)-Human regulator of G-protein signaling 16 (RGS16)

ORF Plasmid: DDK tGFP



  "NM_002928" in other vectors (6)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


RGS16 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms A28-RGS14; A28-RGS14P; RGS-R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202430 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCCGCACCCTGGCCGCCTTCCCCACCACCTGCCTGGAGAGAGCCAAAGAGTTCAAGACACGTCTGG
GGATCTTTCTTCACAAATCAGAGCTGGGCTGCGATACTGGGAGTACTGGCAAGTTCGAGTGGGGCAGTAA
ACACAGCAAAGAGAATAGAAACTTCTCAGAAGATGTGCTGGGGTGGAGAGAGTCGTTCGACCTGCTGCTG
AGCAGTAAAAATGGAGTGGCTGCCTTCCACGCTTTCCTGAAGACAGAGTTCAGTGAGGAGAACCTGGAGT
TCTGGCTGGCCTGTGAGGAGTTCAAGAAGATCCGATCAGCTACCAAGCTGGCCTCCAGGGCACACCAGAT
CTTTGAGGAGTTCATTTGCAGTGAGGCCCCTAAAGAGGTCAACATTGACCATGAGACCCGCGAGCTGACG
AGGATGAACCTGCAGACTGCCACAGCCACATGCTTTGATGCGGCTCAGGGGAAGACACGTACCCTGATGG
AGAAGGACTCCTACCCACGCTTCCTGAAGTCGCCTGCTTACCGGGACCTGGCTGCCCAAGCCTCAGCCGC
CTCTGCCACTCTGTCCAGCTGCAGCCTGGACGAGCCCTCACACACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202430 protein sequence
Red=Cloning site Green=Tags(s)

MCRTLAAFPTTCLERAKEFKTRLGIFLHKSELGCDTGSTGKFEWGSKHSKENRNFSEDVLGWRESFDLLL
SSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKIRSATKLASRAHQIFEEFICSEAPKEVNIDHETRELT
RMNLQTATATCFDAAQGKTRTLMEKDSYPRFLKSPAYRDLAAQASAASATLSSCSLDEPSHT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002928
ORF Size 606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002928.2, NP_002919.2
RefSeq Size 2432 bp
RefSeq ORF 609 bp
Locus ID 6004
UniProt ID O15492
Domains RGS
Protein Families Druggable Genome
MW 22.8 kDa
Gene Summary The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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