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HIF-1 alpha (HIF1A) (NM_001530) Human Tagged ORF Clone
CAT＃: RC202461
HIF1A (Myc-DDK-tagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1
ORF Plasmid: tGFP
"NM_001530" in other vectors (6)
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CNY 6,048.00
| Cited in 18 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202461 representing NM_001530
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAG ATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCC ACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGTGTGAGG AAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGA AAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAA CAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACAC AGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGC AACATGGAAGGTATTGCACTGCACAGGCCACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGT GGGTATAAGAAACCACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTG AAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGA AAGAATTACCGAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCAT GCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACTAAAGGACAAGTCACCACAGGAC AGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAACAC CAAGAATTCTCAACCACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTG ATTTTCTCCCTTCAACAAACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGC TATTCACCAAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTT AACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACT GATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTCCCCTCACCCAACGAAAAATTAC AGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAGCCACTTCGAAGTAGTGCTGA CCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACC ATGCCCCAGATTCAGGATCAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTA ATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTAGA AAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAG ATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTGTCACCATTAG AAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACAGTTACAGTATTCCAGCAGACTCAAAT ACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGAC CGTATGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTG CCACATCATCACCATATAGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCAT AGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGTCAAAGAACTACA GTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGAAAAATGGAAC ATGATGGTTCACTTTTTCAAGCAGTAGGAATTGGAACATTATTACAGCAGCCAGACGATCATGCAGCTAC TACATCACTTTCTTGGAAACGTGTAAAAGGATGCAAATCTAGTGAACAGAATGGAATGGAGCAAAAGACA ATTATTTTAATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGATTACCAC AGCTGACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGA ATTACTCAGAGCTTTGGATCAAGTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202461 representing NM_001530
Red=Cloning site Green=Tags(s) MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVR KLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCD HEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQC GYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYH ALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDL IFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTET DDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFT MPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLE MLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKD RMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTT VPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKT IILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001530 |
| ORF Size | 2478 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001530.4 |
| RefSeq Size | 3958 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 3091 |
| UniProt ID | Q16665 |
| Domains | PAS, HLH, PAC |
| Protein Families | Transcription Factors |
| Protein Pathways | mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma |
| MW | 92.5 kDa |
| Gene Summary | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011] |
Citations (18)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment
,null,
Cancers
,PubMed ID 34572804
[HIF-1 alpha]
|
|
The neural ELAVL protein HuB enhances endogenous proto-oncogene activation
,Hatanaka, T;Higashino, F;Tei, K;Yasuda, M;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31358321
[HIF-1 alpha]
|
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HIF inactivation of p53 in Ovarian Cancer Can be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity
,Parmakhtiar, B;Burger, RA;Kim, JH;Fruehauf, JP;,
Mol. Cancer Res.
,PubMed ID 31088908
[HIF-1 alpha]
|
|
PD-L1 confers resistance to EGFR mutation-independent tyrosine kinase inhibitors in non-small cell lung cancer via upregulation of YAP1 expression
,Tung, JN;Lin, PL;Wang, YC;Wu, DW;Chen, CY;Lee, H;,
Oncotarget
,PubMed ID 29435131
[HIF-1 alpha]
|
|
Role of HIF-1a in regulating autophagic cell survival during cerebral ischemia reperfusion in rats
,Guo, Y;,
Oncotarget
,PubMed ID 29228704
[HIF-1 alpha]
|
|
Functional Analyses of a Novel CITED2 Nonsynonymous Mutation in Chinese Tibetan Patients with Congenital Heart Disease
,Liu, S;Su, Z;Tan, S;Ni, B;Pan, H;Liu, B;Wang, J;Xiao, J;Chen, Q;,
Pediatr Cardiol
,PubMed ID 28687891
[HIF-1 alpha]
|
|
Stemness and differentiation potential-recovery effects of sinapic acid against ultraviolet-A-induced damage through the regulation of p38 MAPK and NF-κB
,Hwang, YS;Park, SH;Kang, M;Oh, SW;Jung, K;Park, YS;Lee, J;,
Sci Rep
,PubMed ID 28424532
[HIF-1 alpha]
|
|
KDM2 Family Members are Regulated by HIF-1 in Hypoxia
,Batie, M;Druker, J;D'Ignazio, L;Rocha, S;,
Cells
,PubMed ID 28304334
[HIF-1 alpha]
|
|
Periostin contributes to arsenic trioxide resistance in hepatocellular carcinoma cells under hypoxia
,Liu, Y;Gao, F;Song, W;,
Biomed. Pharmacother
,PubMed ID 28119236
[HIF-1 alpha]
|
|
Sprouty2 Protein Regulates Hypoxia-inducible Factor-α (HIFα) Protein Levels and Transcription of HIFα-responsive Genes
,Hicks, KC;Patel, TB;,
J. Biol. Chem.
,PubMed ID 27281823
[HIF-1 alpha]
|
|
Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stem cells
,Lee, SY;Park, SH;Kim, MO;Lim, I;Kang, M;Oh, SW;Jung, K;Jo, DG;Cho, IH;Lee, J;,
Food Chem. Toxicol.
,PubMed ID 27470612
[HIF-1 alpha]
|
|
Hypoxia-induced epithelial-mesenchymal transition is regulated by phosphorylation of GSK3-ß via PI3K/Akt signaling in oral squamous cell carcinoma
,Kaneko, T;DDS, ;PhD, ;Dehari, H;DDS, ;PhD, ;Sasaki, T;DDS, ;PhD, ;Igarashi, T;DDS, ;PhD, ;Ogi, K;DDS, ;PhD, ;Okamoto, J;DDS, ;Kawata, M;DDS, ;Kobayashi, J;DDS, ;PhD, ;Miyazaki, A;DDS, ;PhD, ;Nakamori, K;DDS, ;PhD, ;Hiratsuka, H;DDS, ;PhD, ;,
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
[HIF-1 alpha]
|
|
The LINK-A lncRNA activates normoxic HIF1 [alpha] signalling in triple-negative breast cancer
,Lin, A;Li, C;Xing, Z;Hu, Q;Liang, K;Han, L;Wang, C;,
Nature cell biology
,PubMed ID 26751287
[HIF-1 alpha]
|
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CDK dependent phosphorylation of PHD1 on Serine 130 determines specificity in substrate targeting in cells
,Ortmann, B;Bensaddek, D;Carvalhal, S;Moser, SC;Mudie, S;Griffis, ER;Swedlow, JR;Lamond, AI;Rocha, S;,
J. Cell. Sci.
,PubMed ID 26644182
[HIF-1 alpha]
|
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Effect of hypoxia on tissue factor pathway inhibitor expression in breast cancer
,Cui, XY;Tinholt, M;Stavik, B;Dahm, AE;Kanse, S;Jin, Y;Seidl, S;Sahlberg, KK;Iversen, N;Skretting, G;Sandset, PM;OSBREAC Study Group, ;,
J. Thromb. Haemost.
,PubMed ID 26598923
[HIF-1 alpha]
|
|
Snail is a Direct Target of HIF1a in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells
,Xu, X;Tan, X;Tampe, B;Sanchez, E;Zeisberg, M;Zeisberg, EM;,
J. Biol. Chem.
,PubMed ID 25971970
[HIF-1 alpha]
|
|
Enhanced endothelial progenitor cell mobilization and function through direct manipulation of hypoxia inducible factor-1a
,Zan, T;Li, H;Du, Z;Gu, B;Liu, K;Li, Q;,
Cell Biochem. Funct.
,PubMed ID 25801228
[HIF-1 alpha]
|
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Validation of a hypoxia-inducible factor-1 alpha specimen collection procedure and quantitative enzyme-linked immunosorbent assay in solid tumor tissues
,Park, SR;Kinders, RJ;Khin, S;Hollingshead, M;Antony, S;Parchment, RE;Tomaszewski, JE;Kummar, S;Doroshow, JH;,
Anal. Biochem.
,PubMed ID 24799347
[HIF-1 alpha]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202461L1 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, Myc-DDK-tagged |
CNY 8,448.00 |
|
| RC202461L2 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, mGFP tagged |
CNY 8,448.00 |
|
| RC202461L3 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, Myc-DDK-tagged |
CNY 8,448.00 |
|
| RC202461L4 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG202461 | HIF1A (tGFP-tagged) - Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1 |
CNY 7,648.00 |
|
| SC119189 | HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1 |
CNY 9,088.00 |
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