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EPHX2 (NM_001979) Human Tagged ORF Clone
CAT＃: RC202489
EPHX2 (Myc-DDK-tagged)-Human epoxide hydrolase 2, cytoplasmic (EPHX2)
ORF Plasmid: tGFP
"NM_001979" in other vectors (6)
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CNY 4,136.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABHD20; CEH; SEH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202489 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCTGCGCGCGGCCGTCTTCGACCTTGACGGGGTGCTGGCGCTGCCAGCGGTGTTCGGCGTCCTCG GCCGCACGGAGGAGGCCCTGGCGCTGCCCAGAGGACTTCTGAATGATGCTTTCCAGAAAGGGGGACCAGA GGGTGCCACTACCCGGCTTATGAAAGGAGAGATCACACTTTCCCAGTGGATACCACTCATGGAAGAAAAC TGCAGGAAGTGCTCCGAGACCGCTAAAGTCTGCCTCCCCAAGAATTTCTCCATAAAAGAAATCTTTGACA AGGCGATTTCAGCCAGAAAGATCAACCGCCCCATGCTCCAGGCAGCTCTCATGCTCAGGAAGAAAGGATT CACTACTGCCATCCTCACCAACACCTGGCTGGACGACCGTGCTGAGAGAGATGGCCTGGCCCAGCTGATG TGTGAGCTGAAGATGCACTTTGACTTCCTGATAGAGTCGTGTCAGGTGGGAATGGTCAAACCTGAACCTC AGATCTACAAGTTTCTGCTGGACACCCTGAAGGCCAGCCCCAGTGAGGTCGTTTTTTTGGATGACATCGG GGCTAATCTGAAGCCAGCCCGTGACTTGGGAATGGTCACCATCCTGGTCCAGGACACTGACACGGCCCTG AAAGAACTGGAGAAAGTGACCGGAATCCAGCTTCTCAATACCCCGGCCCCTCTGCCGACCTCTTGCAATC CAAGTGACATGAGCCATGGGTACGTGACAGTAAAGCCCAGGGTCCGTCTGCATTTTGTGGAGCTGGGCTC CGGCCCTGCTGTGTGCCTCTGCCATGGATTTCCCGAGAGTTGGTATTCTTGGAGGTACCAGATCCCTGCT CTGGCCCAGGCAGGTTACCGGGTCCTAGCTATGGACATGAAAGGCTATGGAGAGTCATCTGCTCCTCCCG AAATAGAAGAATATTGCATGGAAGTGTTATGTAAGGAGATGGTAACCTTCCTGGATAAACTGGGCCTCTC TCAAGCAGTGTTCATTGGCCATGACTGGGGTGGCATGCTGGTGTGGTACATGGCTCTCTTCTACCCCGAG AGAGTGAGGGCGGTGGCCAGTTTGAATACTCCCTTCATACCAGCAAATCCCAACATGTCCCCTTTGGAGA GTATCAAAGCCAACCCAGTATTTGATTACCAGCTCTACTTCCAAGAACCAGGAGTGGCTGAGGCTGAACT GGAACAGAACCTGAGTCGGACTTTCAAAAGCCTCTTCAGAGCAAGCGATGAGAGTGTTTTATCCATGCAT AAAGTCTGTGAAGCGGGAGGACTTTTTGTAAATAGCCCAGAAGAGCCCAGCCTCAGCAGGATGGTCACTG AGGAGGAAATCCAGTTCTATGTGCAGCAGTTCAAGAAGTCTGGTTTCAGAGGTCCTCTAAACTGGTACCG AAACATGGAAAGGAACTGGAAGTGGGCTTGCAAAAGCTTGGGACGGAAGATCCTGATTCCGGCCCTGATG GTCACGGCGGAGAAGGACTTCGTGCTCGTTCCTCAGATGTCCCAGCACATGGAGGACTGGATTCCCCACC TGAAAAGGGGACACATTGAGGACTGTGGGCACTGGACACAGATGGACAAGCCAACCGAGGTGAATCAGAT CCTCATTAAGTGGCTGGATTCTGATGCCCGGAACCCACCGGTGGTCTCAAAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202489 protein sequence
Red=Cloning site Green=Tags(s) MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEEN CRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLM CELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTAL KELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPA LAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPE RVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMH KVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALM VTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001979 |
| ORF Size | 1665 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001979.6 |
| RefSeq Size | 2290 bp |
| RefSeq ORF | 1668 bp |
| Locus ID | 2053 |
| UniProt ID | P34913 |
| Domains | abhydrolase, Hydrolase |
| Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
| MW | 62.6 kDa |
| Gene Summary | This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Novel Endocrine Role the BAT-Released Lipokine 12,13-diHOME to Mediate Cardiac Function
,Pinckard, KM;Shettigar, VK;Wright, KR;Abay, E;Baer, LA;Vidal, P;Dewal, RS;Das, D;Duarte-Sanmiguel, S;Hernández-Saavedra, D;Arts, PJ;Lehnig, AC;Bussberg, V;Narain, NR;Kiebish, MA;Yi, F;Sparks, LM;Goodpaster, BH;Smith, SR;Pratley, RE;Lewandowski, ED;Raman, SV;Wold, LE;Gallego-Perez, D;Coen, PM;Ziolo, MT;Stanford, KI;,
Circulation
,PubMed ID 33106031
[EPHX2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202489L1 | Lenti ORF clone of Human epoxide hydrolase 2, cytoplasmic (EPHX2), Myc-DDK-tagged |
CNY 6,536.00 |
|
| RC202489L2 | Lenti ORF clone of Human epoxide hydrolase 2, cytoplasmic (EPHX2), mGFP tagged |
CNY 6,460.00 |
|
| RC202489L3 | Lenti ORF clone of Human epoxide hydrolase 2, cytoplasmic (EPHX2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202489L4 | Lenti ORF clone of Human epoxide hydrolase 2, cytoplasmic (EPHX2), mGFP tagged |
CNY 6,460.00 |
|
| RG202489 | EPHX2 (tGFP-tagged) - Human epoxide hydrolase 2, cytoplasmic (EPHX2) |
CNY 5,736.00 |
|
| SC319259 | EPHX2 (untagged)-Human epoxide hydrolase 2, cytoplasmic (EPHX2) |
CNY 4,144.00 |
