United States
Germany
Japan
United Kingdom
China
Elastase (CELA3B) (NM_007352) Human Tagged ORF Clone
CAT＃: RC202678
- TrueORF®
CELA3B (Myc-DDK-tagged)-Human chymotrypsin-like elastase family, member 3B (CELA3B)
ORF Plasmid: tGFP
"NM_007352" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CBPP; ELA3B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202678 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTCCGGCTGCTCAGTTCCCTCCTCCTTGTGGCCGTTGCCTCAGGCTATGGCCCACCTTCCTCTC GCCCTTCCAGCCGCGTTGTCAATGGTGAGGATGCGGTCCCCTACAGCTGGCCCTGGCAGGTTTCCCTGCA GTATGAGAAAAGTGGAAGCTTCTACCACACGTGTGGCGGTAGCCTCATCGCCCCCGACTGGGTTGTGACT GCCGGCCACTGCATCTCGAGCTCCTGGACCTACCAGGTGGTGTTGGGCGAGTACGACCGTGCTGTGAAGG AGGGCCCCGAGCAGGTGATCCCCATCAACTCTGGGGACCTCTTTGTGCATCCACTCTGGAACCGCTCGTG TGTGGCCTGTGGCAATGACATCGCCCTCATCAAGCTCTCACGCAGCGCCCAGCTGGGAGACGCCGTCCAG CTCGCCTCACTCCCTCCCGCTGGTGACATCCTTCCCAACGAGACACCCTGCTACATCACCGGCTGGGGCC GTCTCTATACCAACGGGCCACTCCCAGACAAGCTGCAGGAGGCCCTGCTGCCCGTGGTGGACTATGAACA CTGCTCCAGGTGGAACTGGTGGGGTTCCTCCGTGAAGAAGACCATGGTGTGTGCTGGAGGGGACATCCGC TCCGGCTGCAACGGTGACTCTGGAGGACCCCTCAACTGCCCCACAGAGGATGGTGGCTGGCAGGTCCATG GCGTGACCAGCTTTGTTTCTGCCTTTGGCTGCAACACCCGCAGGAAGCCCACGGTGTTCACTCGAGTCTC CGCCTTCATCGACTGGATTGAGGAGACCATAGCAAGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202678 protein sequence
Red=Cloning site Green=Tags(s) MMLRLLSSLLLVAVASGYGPPSSRPSSRVVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVT AGHCISSSWTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQ LASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIR SGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007352 |
| ORF Size | 810 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007352.4 |
| RefSeq Size | 1055 bp |
| RefSeq ORF | 813 bp |
| Locus ID | 23436 |
| UniProt ID | P08861 |
| Protein Families | Druggable Genome, Protease |
| MW | 29.3 kDa |
| Gene Summary | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202678L3 | Lenti ORF clone of Human chymotrypsin-like elastase family, member 3B (CELA3B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202678L4 | Lenti ORF clone of Human chymotrypsin-like elastase family, member 3B (CELA3B), mGFP tagged |
CNY 5,890.00 |
|
| RG202678 | CELA3B (tGFP-tagged) - Human chymotrypsin-like elastase family, member 3B (CELA3B) |
CNY 4,000.00 |
|
| SC125570 | CELA3B (untagged)-Human chymotrypsin-like elastase family, member 3B (CELA3B) |
CNY 6,270.00 |
其它Elastase产品
