TINP1 (NSA2) (NM_014886) Human Tagged ORF Clone
CAT#: RC202728
NSA2 (Myc-DDK-tagged)-Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2)
ORF Plasmid: tGFP
"NM_014886" in other vectors (5)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDK105; HCL-G1; HCLG1; HUSSY-29; HUSSY29; TINP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202728 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACAGAATGAATATATTGAATTACACCGTAAACGCTATGGATACCGTTTGGATTACCATGAGAAAA AGAGAAAGAAGGAAAGTCGAGAGGCTCATGAACGTTCAAAGAAGGCAAAGAAAATGATTGGTCTGAAGGC TAAGCTTTACCATAAACAGCGTCATGCTGAGAAAATACAAATGAAAAAGACTATCAAGATGCATGAAAAG AGAAACACCAAACAAAAGAATGATGAAAAGACACCACAGGGAGCAGTACCTGCCTATCTGCTGGACAGAG AGGGACAATCTCGAGCTAAAGTACTTTCCAATATGATTAAACAGAAAAGAAAAGAGAAGGCGGGAAAATG GGAAGTCCCTCTGCCTAAAGTACGTGCCCAGGGAGAAACAGAAGTATTAAAAGTTATTCGAACAGGAAAG AGAAAGAAGAAGGCATGGAAGAGAATGGTTACTAAAGTGTGCTTTGTTGGAGATGGCTTTACAAGAAAAC CACCTAAATATGAAAGATTCATCAGGCCAATGGGCTTGCGTTTCAAGAAAGCCCATGTAACACATCCTGA ACTGAAAGCCACCTTTTGCCTACCAATACTTGGTGTAAAGAAGAATCCCTCATCCCCACTGTATACAACT TTGGGTGTTATTACCAAAGGTACTGTCATTGAAGTAAATGTGAGCGAATTGGGCCTTGTGACACAAGGAG GCAAAGTTATTTGGGGAAAATATGCCCAGGTTACCAACAATCCTGAAAATGATGGATGTATAAATGCAGT CTTACTGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202728 protein sequence
Red=Cloning site Green=Tags(s) MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEK RNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGK RKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTT LGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINAVLLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014886 |
ORF Size | 780 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014886.6 |
RefSeq Size | 1401 bp |
RefSeq ORF | 783 bp |
Locus ID | 10412 |
UniProt ID | O95478 |
Domains | Ribosomal_S8e |
MW | 30.1 kDa |
Gene Summary | This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202728L3 | Lenti ORF clone of Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202728L4 | Lenti ORF clone of Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2), mGFP tagged |
CNY 5,890.00 |
|
RG202728 | NSA2 (tGFP-tagged) - Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2) |
CNY 4,000.00 |
|
SC114735 | NSA2 (untagged)-Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2) |
CNY 2,400.00 |
|
SC321061 | NSA2 (untagged)-Human NSA2 ribosome biogenesis homolog (S. cerevisiae) (NSA2) |
CNY 2,400.00 |