United States
Germany
Japan
United Kingdom
China
RFK (NM_018339) Human Tagged ORF Clone
CAT＃: RC202812
RFK (Myc-DDK-tagged)-Human riboflavin kinase (RFK)
ORF Plasmid: tGFP
"NM_018339" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RIFK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGAGCGGACTGCATTATGAGGCACCTGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCG GCCGCGGCTCCAAGCAGCTGGGCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGC TGATATATCCACTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAGATGGTGGTG AGCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACACATATCATGCATACCTTCA AAGAGGACTTCTATGGGGAAATCCTCAATGTCGCCATTGTTGGCTACCTGAGACCAGAAAAGAACTTTGA TTCTTTAGAGTCACTTATTTCAGCAATTCAAGGTGATATTGAAGAAGCTAAGAAACGACTAGAGTTACCA GAACATTTGAAAATCAAAGAAGACAATTTCTTCCAGGTTTCTAAAAGCAAAATAATGAATGGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202812 protein sequence
Red=Cloning site Green=Tags(s) MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVV SIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELP EHLKIKEDNFFQVSKSKIMNGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018339 |
| ORF Size | 486 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018339.2 |
| RefSeq Size | 2707 bp |
| RefSeq ORF | 468 bp |
| Locus ID | 55312 |
| UniProt ID | Q969G6 |
| Domains | FAD_Synth |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Riboflavin metabolism |
| MW | 18.4 kDa |
| Gene Summary | Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202812L3 | Lenti ORF clone of Human riboflavin kinase (RFK), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202812L4 | Lenti ORF clone of Human riboflavin kinase (RFK), mGFP tagged |
CNY 5,890.00 |
|
| RG202812 | RFK (tGFP-tagged) - Human riboflavin kinase (RFK) |
CNY 2,800.00 |
|
| SC113557 | RFK (untagged)-Human riboflavin kinase (RFK) |
CNY 1,200.00 |
