C11orf2 (VPS51) (NM_013265) Human Tagged ORF Clone

CAT#: RC202820

VPS51 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 2 (C11orf2)

ORF Plasmid: DDK tGFP



  "NM_013265" in other vectors (4)

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CNY 8,592.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


VPS51 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ANG2; ANG3; C11orf2; C11orf3; FFR; PCH13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202820 representing NM_013265
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCAGCTGCCGCCGGGCCTAGCCCGGGGTCTGGACCTGGGGACTCCCCAGAAGGGCCCGAGG
GGGAGGCTCCGGAGCGTCGGCGGAAGGCGCACGGGATGCTGAAGCTTTACTACGGCCTCTCGGAAGGGGA
GGCGGCGGGACGCCCCGCGGGGCCCGACCCCCTGGACCCGACTGATCTGAACGGGGCGCACTTCGACCCG
GAAGTTTACCTAGACAAGCTGCGTAGAGAGTGCCCTCTGGCCCAGTTGATGGACAGTGAGACGGACATGG
TGCGGCAGATCCGGGCTCTAGACAGCGACATGCAGACCCTGGTCTATGAGAACTACAACAAGTTCATCTC
AGCCACAGACACCATCCGGAAGATGAAGAACGATTTCCGGAAGATGGAGGATGAGATGGACCGGCTGGCC
ACCAACATGGCAGTGATCACCGACTTCAGCGCTCGCATCAGCGCCACGCTGCAGGACCGCCACGAGCGCA
TCACCAAGCTGGCAGGGGTCCACGCGCTGCTGCGGAAGCTGCAGTTCCTCTTTGAGCTGCCCTCGCGCCT
CACCAAGTGCGTGGAACTGGGCGCCTATGGGCAGGCGGTGCGCTACCAGGGCCGCGCGCAGGCCGTGCTG
CAGCAGTACCAACACCTGCCCTCGTTCCGCGCCATCCAGGACGACTGCCAGGTCATCACGGCCCGCCTGG
CCCAGCAGCTGCGGCAGCGCTTTAGGGAGGGCGGCTCAGGCGCCCCGGAGCAGGCAGAGTGCGTGGAGCT
GCTGCTGGCCCTGGGCGAGCCTGCGGAGGAGCTGTGCGAGGAGTTCCTGGCGCACGCCCGCGGCCGGCTG
GAGAAGGAGCTGAGAAACCTGGAGGCCGAGCTGGGGCCCTCACCTCCGGCTCCCGACGTGTTAGAGTTCA
CCGACCATGGAGGCAGTGGCTTCGTGGGCGGCCTCTGCCAGGTGGCGGCGGCCTACCAGGAGCTGTTTGC
GGCCCAGGGCCCAGCAGGTGCCGAGAAGCTGGCGGCCTTCGCCCGGCAGCTGGGCAGCCGCTATTTTGCG
CTGGTGGAGCGGCGGCTGGCGCAGGAGCAGGGTGGTGGTGACAACTCACTGCTGGTGCGGGCGCTGGACC
GCTTCCACCGGCGCTTGCGGGCTCCCGGGGCCCTGCTGGCCGCTGCCGGGCTCGCAGACGCTGCCACGGA
GATCGTGGAACGAGTGGCCCGCGAGCGCCTGGGCCACCACCTGCAGGGTCTCCGGGCGGCCTTCCTGGGC
TGCCTGACAGACGTCCGCCAGGCGCTGGCAGCACCTCGCGTGGCTGGGAAGGAGGGCCCTGGCCTGGCCG
AGTTGCTGGCCAATGTGGCCAGCTCCATCCTGAGCCACATTAAGGCCTCTCTGGCAGCAGTGCACCTTTT
CACCGCCAAAGAGGTGTCCTTCTCCAACAAGCCCTACTTCCGGGGTGAGTTCTGCAGTCAGGGTGTCCGT
GAGGGCCTCATCGTGGGCTTCGTCCACTCTATGTGCCAGACGGCTCAGAGCTTCTGCGACAGCCCTGGGG
AGAAGGGGGGTGCCACACCACCTGCCCTGCTCCTGCTGCTCTCCCGCCTCTGCCTGGACTACGAGACGGC
CACCATCTCCTACATCCTCACTCTCACTGATGAACAGTTTCTGGTGCAGGATCAGTTCCCAGTGACGCCC
GTGAGCACGCTGTGTGCAGAGGCCAGGGAAACGGCGCGGCGGCTGCTGACCCACTACGTGAAGGTGCAGG
GCCTGGTCATATCACAGATGCTGCGCAAGAGCGTGGAGACTCGCGACTGGCTCAGCACTCTGGAGCCCCG
GAATGTGCGGGCCGTCATGAAGCGGGTGGTGGAGGATACCACCGCCATCGACGTGCAGGTGGGGCTCCTG
TACGAAGAGGGTGTTCGCAAGGCCCAGAGCAGCGACTCCAGCAAGAGGACTTTCTCCGTGTACAGCAGCT
CTCGGCAGCAGGGCCGCTACGCCCCCAGCTATACCCCCAGTGCCCCGATGGACACCAACCTCTTGAGCAA
TATCCAGAAGCTATTCTCTGAACGTATTGATGTGTTCAGCCCTGTGGAGTTCAACAAGGTGTCGGTGCTG
ACCGGCATCATCAAGATCAGCCTGAAGACGCTGCTGGAGTGTGTGCGGCTGCGCACCTTTGGGCGCTTCG
GGCTGCAGCAGGTGCAAGTGGACTGCCACTTTCTGCAGCTCTACCTGTGGCGTTTTGTGGCCGACGAAGA
ACTCGTGCACTTGCTGCTGGACGAAGTGGTGGCCTCTGCTGCCCTGCGCTGCCCAGACCCTGTGCCCATG
GAGCCCAGTGTGGTTGAGGTCATCTGCGAGCGCGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202820 representing NM_013265
Red=Cloning site Green=Tags(s)

MAAAAAAGPSPGSGPGDSPEGPEGEAPERRRKAHGMLKLYYGLSEGEAAGRPAGPDPLDPTDLNGAHFDP
EVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLA
TNMAVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRAQAVL
QQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELLLALGEPAEELCEEFLAHARGRL
EKELRNLEAELGPSPPAPDVLEFTDHGGSGFVGGLCQVAAAYQELFAAQGPAGAEKLAAFARQLGSRYFA
LVERRLAQEQGGGDNSLLVRALDRFHRRLRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLG
CLTDVRQALAAPRVAGKEGPGLAELLANVASSILSHIKASLAAVHLFTAKEVSFSNKPYFRGEFCSQGVR
EGLIVGFVHSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQFPVTP
VSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLL
YEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVL
TGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPM
EPSVVEVICERG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013265
ORF Size 2346 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013265.4
RefSeq Size 2546 bp
RefSeq ORF 2349 bp
Locus ID 738
UniProt ID Q9UID3
MW 85.9 kDa
Gene Summary This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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