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PLA2G12A (NM_030821) Human Tagged ORF Clone
CAT＃: RC202955
PLA2G12A (Myc-DDK-tagged)-Human phospholipase A2, group XIIA (PLA2G12A)
ORF Plasmid: tGFP
"NM_030821" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GXII; PLA2G12; ROSSY |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202955 representing NM_030821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGCTCTCGCGCCCCGCGCTCACCCTCCTGCTCCTCCTCATGGCCGCTGTTGTCAGGTGCCAGG AGCAGGCCCAGACCACCGACTGGAGAGCCACCCTGAAGACCATCCGGAACGGCGTTCATAAGATAGACAC GTACCTGAACGCCGCCTTGGACCTCCTGGGAGGCGAGGACGGTCTCTGCCAGTATAAATGCAGTGACGGA TCTAAGCCTTTCCCACGTTATGGTTATAAACCCTCCCCACCGAATGGATGTGGCTCTCCACTGTTTGGTG TTCATCTTAACATTGGTATCCCTTCCCTGACAAAGTGTTGCAACCAACACGACAGGTGCTATGAGACCTG TGGCAAAAGCAAGAATGACTGTGATGAAGAATTCCAGTATTGCCTCTCCAAGATCTGCCGAGATGTACAG AAAACACTAGGACTAACTCAGCATGTTCAGGCATGTGAAACAACAGTGGAGCTCTTGTTTGACAGTGTTA TACATTTAGGTTGTAAACCATATCTGGACAGCCAACGAGCCGCATGCAGGTGTCATTATGAAGAAAAAAC TGATCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202955 representing NM_030821
Red=Cloning site Green=Tags(s) MALLSRPALTLLLLLMAAVVRCQEQAQTTDWRATLKTIRNGVHKIDTYLNAALDLLGGEDGLCQYKCSDG SKPFPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICRDVQ KTLGLTQHVQACETTVELLFDSVIHLGCKPYLDSQRAACRCHYEEKTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030821 |
| ORF Size | 567 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030821.5 |
| RefSeq Size | 1699 bp |
| RefSeq ORF | 570 bp |
| Locus ID | 81579 |
| UniProt ID | Q9BZM1 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway |
| MW | 20.9 kDa |
| Gene Summary | Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202955L1 | Lenti ORF clone of Human phospholipase A2, group XIIA (PLA2G12A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202955L2 | Lenti ORF clone of Human phospholipase A2, group XIIA (PLA2G12A), mGFP tagged |
CNY 5,890.00 |
|
| RC202955L3 | Lenti ORF clone of Human phospholipase A2, group XIIA (PLA2G12A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202955L4 | Lenti ORF clone of Human phospholipase A2, group XIIA (PLA2G12A), mGFP tagged |
CNY 5,890.00 |
|
| RG202955 | PLA2G12A (tGFP-tagged) - Human phospholipase A2, group XIIA (PLA2G12A) |
CNY 4,370.00 |
|
| SC109014 | PLA2G12A (untagged)-Human phospholipase A2, group XIIA (PLA2G12A) |
CNY 2,400.00 |
|
| SC320348 | PLA2G12A (untagged)-Human phospholipase A2, group XIIA (PLA2G12A) |
CNY 2,400.00 |
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