PSMD7 (NM_002811) Human Tagged ORF Clone
CAT#: RC203133
PSMD7 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7)
ORF Plasmid: tGFP
"NM_002811" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MOV34; P40; Rpn8; S12 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203133 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGCTGGCAGTGCAGAAGGTGGTGGTCCACCCCCTGGTGCTGCTCAGTGTGGTGGATCATTTCA ACCGAATCGGCAAGGTTGGAAACCAGAAGCGTGTTGTTGGTGTGCTTTTGGGGTCATGGCAAAAGAAAGT ACTTGATGTATCGAACAGTTTTGCAGTTCCTTTTGATGAAGATGACAAAGACGATTCTGTATGGTTTTTA GACCATGATTATTTGGAAAACATGTATGGAATGTTTAAGAAAGTCAATGCCAGGGAAAGAATAGTTGGCT GGTACCACACAGGCCCTAAACTACACAAGAATGACATTGCCATCAACGAACTCATGAAAAGATACTGTCC TAATTCCGTATTGGTCATCATTGATGTGAAGCCGAAGGACCTAGGGCTGCCTACAGAAGCGTACATTTCA GTGGAAGAAGTCCATGATGATGGAACTCCAACCTCGAAAACATTTGAACACGTGACCAGTGAAATTGGAG CAGAGGAAGCTGAGGAAGTTGGAGTTGAACACTTGTTACGAGATATCAAAGACACGACGGTGGGCACTCT GTCCCAGCGGATCACAAACCAGGTCCATGGTTTGAAGGGACTGAACTCCAAGCTTCTGGATATCAGGAGC TACCTGGAAAAAGTCGCCACAGGCAAGCTGCCCATCAACCACCAGATCATCTACCAGCTGCAGGACGTCT TCAACCTGCTGCCAGATGTCAGCCTGCAGGAGTTCGTCAAGGCCTTTTACCTGAAGACCAATGACCAGAT GGTGGTAGTGTACTTGGCCTCGCTGATCCGTTCCGTGGTCGCCCTGCACAACCTCATCAACAACAAGATT GCCAACCGGGATGCAGAGAAGAAAGAAGGGCAGGAGAAAGAAGAGAGCAAAAAGGATAGGAAAGAGGACA AGGAGAAAGATAAAGATAAGGAAAAGAGTGATGTAAAGAAAGAGGAGAAAAAGGAGAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203133 protein sequence
Red=Cloning site Green=Tags(s) MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFL DHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYIS VEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRS YLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKI ANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEEKKEKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002811 |
ORF Size | 972 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002811.5 |
RefSeq Size | 1686 bp |
RefSeq ORF | 975 bp |
Locus ID | 5713 |
UniProt ID | P51665 |
Domains | JAB_MPN |
Protein Families | Druggable Genome, Protease |
Protein Pathways | Proteasome |
MW | 37 kDa |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Ubiquitin Pathway Is Associated with Worsening Left Ventricle Function after Mitral Valve Repair: A Global Gene Expression Study
,Tsai, FC;Chang, GJ;Lai, YJ;Chang, SH;Chen, WJ;Yeh, YH;,
Int J Mol Sci
,PubMed ID 32708358
[PSMD7]
|
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome
,Yi, JJ;Paranjape, SR;Walker, MP;Choudhury, R;Wolter, JM;Fragola, G;Emanuele, MJ;Major, MB;Zylka, MJ;,
J. Biol. Chem.
,PubMed ID 28559284
[PSMD7]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203133L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203133L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7), mGFP tagged |
CNY 5,890.00 |
|
RG203133 | PSMD7 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7) |
CNY 4,000.00 |
|
SC118382 | PSMD7 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7) |
CNY 2,400.00 |
|
SC320876 | PSMD7 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7) |
CNY 2,400.00 |