MARCKS like protein (MARCKSL1) (NM_023009) Human Tagged ORF Clone
CAT#: RC203181
MARCKSL1 (Myc-DDK-tagged)-Human MARCKS-like 1 (MARCKSL1)
ORF Plasmid: tGFP
"NM_023009" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | F52; MACMARCKS; MLP; MLP1; MRP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCCAGAGCTCCAAGGCTCCCCGGGGCGACGTGACCGCCGAGGAGGCAGCAGGCGCTTCCCCCG CGAAGGCCAACGGCCAGGAGAATGGCCACGTGAAAAGCAATGGAGACTTATCCCCCAAGGGTGAAGGGGA GTCGCCCCCTGTGAACGGAACAGATGAGGCAGCCGGGGCCACTGGCGATGCCATCGAGCCAGCACCCCCT AGCCAGGGTGCTGAGGCCAAGGGGGAGGTCCCCCCCAAGGAGACCCCCAAGAAGAAGAAGAAATTCTCTT TCAAGAAGCCTTTCAAATTGAGCGGCCTGTCCTTCAAGAGAAATCGGAAGGAGGGTGGGGGTGATTCTTC TGCCTCCTCACCCACAGAGGAAGAGCAGGAGCAGGGGGAGATCGGTGCCTGCAGCGACGAGGGCACTGCT CAGGAAGGGAAGGCCGCAGCCACCCCTGAGAGCCAGGAACCCCAGGCCAAGGGGGCAGAGGCTAGTGCAG CCTCAGAAGAAGAGGCAGGGCCCCAGGCTACAGAGCCATCCACTCCCTCGGGGCCGGAGAGTGGCCCTAC ACCAGCCAGCGCTGAGCAGAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203181 protein sequence
Red=Cloning site Green=Tags(s) MGSQSSKAPRGDVTAEEAAGASPAKANGQENGHVKSNGDLSPKGEGESPPVNGTDEAAGATGDAIEPAPP SQGAEAKGEVPPKETPKKKKKFSFKKPFKLSGLSFKRNRKEGGGDSSASSPTEEEQEQGEIGACSDEGTA QEGKAAATPESQEPQAKGAEASAASEEEAGPQATEPSTPSGPESGPTPASAEQNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_023009 |
ORF Size | 585 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023009.7 |
RefSeq Size | 1562 bp |
RefSeq ORF | 588 bp |
Locus ID | 65108 |
UniProt ID | P49006 |
Domains | MARCKS |
Protein Pathways | Fc gamma R-mediated phagocytosis |
MW | 19.5 kDa |
Gene Summary | This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203181L3 | Lenti ORF clone of Human MARCKS-like 1 (MARCKSL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203181L4 | Lenti ORF clone of Human MARCKS-like 1 (MARCKSL1), mGFP tagged |
CNY 5,890.00 |
|
RG203181 | MARCKSL1 (tGFP-tagged) - Human MARCKS-like 1 (MARCKSL1) |
CNY 4,000.00 |
|
SC112373 | MARCKSL1 (untagged)-Human MARCKS-like 1 (MARCKSL1) |
CNY 2,400.00 |
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