SQSTM1 (NM_003900) Human Tagged ORF Clone

Name: SQSTM1 (NM_003900) Human Tagged ORF Clone
SKU: RC203214
Price: 5488.00

CAT＃: RC203214

SQSTM1 (Myc-DDK-tagged)-Human sequestosome 1 (SQSTM1), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_003900" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 5,488.00

货期*

现货

规格

Cited in 9 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA502127

SQSTM1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A170; DMRV; FTDALS3; NADGP; OSIL; p60; p62; p62B; PDB3; ZIP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC203214 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGCTCACCGTGAAGGCCTACCTTCTGGGCAAGGAGGACGCGGCGCGCGAGATTCGCCGCTTCA GCTTCTGCTGCAGCCCCGAGCCTGAGGCGGAAGCCGAGGCTGCGGCGGGTCCGGGACCCTGCGAGCGGCT GCTGAGCCGGGTGGCCGCCCTGTTCCCCGCGCTGCGGCCTGGCGGCTTCCAGGCGCACTACCGCGATGAG GACGGGGACTTGGTTGCCTTTTCCAGTGACGAGGAATTGACAATGGCCATGTCCTACGTGAAGGATGACA TCTTCCGAATCTACATTAAAGAGAAAAAAGAGTGCCGGCGGGACCACCGCCCACCGTGTGCTCAGGAGGC GCCCCGCAACATGGTGCACCCCAATGTGATCTGCGATGGCTGCAATGGGCCTGTGGTAGGAACCCGCTAC AAGTGCAGCGTCTGCCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGGGGCACA CCAAGCTCGCATTCCCCAGCCCCTTCGGGCACCTGTCTGAGGGCTTCTCGCACAGCCGCTGGCTCCGGAA GGTGAAACACGGACACTTCGGGTGGCCAGGATGGGAAATGGGTCCACCAGGAAACTGGAGCCCACGTCCT CCTCGTGCAGGGGAGGCCCGCCCTGGCCCCACGGCAGAATCAGCTTCTGGTCCATCGGAGGATCCGAGTG TGAATTTCCTGAAGAACGTTGGGGAGAGTGTGGCAGCTGCCCTTAGCCCTCTGGGCATTGAAGTTGATAT CGATGTGGAGCACGGAGGGAAAAGAAGCCGCCTGACCCCCGTCTCTCCAGAGAGTTCCAGCACAGAGGAG AAGAGCAGCTCACAGCCAAGCAGCTGCTGCTCTGATCCCAGCAAGCCGGGTGGGAATGTTGAGGGCGCCA CGCAGTCTCTGGCGGAGCAGATGAGAAAGATCGCCTTGGAGTCCGAGGGGCGCCCTGAGGAACAGATGGA GTCGGATAACTGTTCAGGAGGAGATGATGACTGGACCCATCTGTCTTCAAAAGAAGTGGACCCGTCTACA GGTGAACTCCAGTCCCTACAGATGCCAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGAC CCACAGGGCTGAAGGAAGCTGCCTTGTACCCACATCTCCCGCCAGAGGCTGACCCGCGGCTGATTGAGTC CCTCTCCCAGATGCTGTCCATGGGCTTCTCTGATGAAGGCGGCTGGCTCACCAGGCTCCTGCAGACCAAG AACTATGACATCGGAGCGGCTCTGGACACCATCCAGTATTCAAAGCATCCCCCGCCGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC203214 protein sequence Red=Cloning site Green=Tags(s) MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDE DGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRY KCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRP PRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEE KSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPST GELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTK NYDIGAALDTIQYSKHPPPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_003900
ORF Size	1320 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003900.2
RefSeq Size	2923 bp
RefSeq ORF	1323 bp
Locus ID	8878
UniProt ID	Q13501
Domains	PB1, ZnF_ZZ, UBA
Protein Families	Druggable Genome, Transcription Factors
MW	47.7 kDa
Gene Summary	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (9)

The use of this cDNA Clones has been cited in the following citations:
The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKK α and IKK β through the recruitment of TOLLIP ,null, PLOS Pathogens ,PubMed ID 37566637 [SQSTM1]
AKR1C1 connects autophagy and oxidative stress by interacting with SQSTM1 in a catalytic-independent manner ,Chang, LL;Li, YK;Zhao, CX;Zeng, CM;Ge, FJ;Du, JM;Zhang, WZ;Lu, PH;He, QJ;Zhu, H;Yang, B;, Acta pharmacologica Sinica ,PubMed ID 34017066 [SQSTM1]
EGFR enhances the stemness and progression of oral cancer through inhibiting autophagic degradation of SOX2 ,Lv, XX;Zheng, XY;Yu, JJ;Ma, HR;Hua, C;Gao, RT;, Cancer Med ,PubMed ID 31823521 [SQSTM1]
SFTS virus NSs interacts with TRIM21 to activate the p62-Keap1-Nrf2 pathway ,Choi, Y;Jiang, Z;Shin, WJ;Jung, JU;, J. Virol. ,PubMed ID 31852783 [SQSTM1]
A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism. ,null, Acta neuropathologica ,PubMed ID 29302778 [SQSTM1]
HMGB1 represses the anti-cancer activity of sunitinib by governing TP53 autophagic degradation via its nucleus-to-cytoplasm transport ,Luo, P;Xu, Z;Li, G;Yan, H;Zhu, Y;Zhu, H;Ma, S;Yang, B;He, Q;, Autophagy ,PubMed ID 30205729 [SQSTM1]
A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition ,Song, JX;Sun, YR;Peluso, I;Zeng, Y;Yu, X;Lu, JH;Xu, Z;Wang, MZ;Liu, LF;Huang, YY;Chen, LL;Durairajan, SS;Zhang, HJ;Zhou, B;Zhang, HQ;Lu, A;Ballabio, A;Medina, DL;Guo, Z;Li, M;, Autophagy ,PubMed ID 27172265 [SQSTM1]
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death ,Sellier, C;Campanari, ML;Julie Corbier, C;Gaucherot, A;Kolb-Cheynel, I;Oulad-Abdelghani, M;Ruffenach, F;Page, A;Ciura, S;Kabashi, E;Charlet-Berguerand, N;, EMBO J. ,PubMed ID 27103069 [SQSTM1]
Proteasome inhibition and oxidative reactions disrupt cellular homeostasis during heme stress ,Vallelian, F;Deuel, JW;Opitz, L;Schaer, CA;Puglia, M;Lönn, M;Engelsberger, W;Schauer, S;Karnaukhova, E;Spahn, DR;Stocker, R;Buehler, PW;Schaer, DJ;, Cell Death Differ. ,PubMed ID 25301065 [SQSTM1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC203214L1	Lenti ORF clone of Human sequestosome 1 (SQSTM1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC203214L2	Lenti ORF clone of Human sequestosome 1 (SQSTM1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC203214L3	Lenti ORF clone of Human sequestosome 1 (SQSTM1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC203214L4	Lenti ORF clone of Human sequestosome 1 (SQSTM1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RG203214	SQSTM1 (tGFP-tagged) - Human sequestosome 1 (SQSTM1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,088.00
SC117669	SQSTM1 (untagged)-Human sequestosome 1 (SQSTM1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,488.00