MAD2 (MAD2L1) (NM_002358) Human Tagged ORF Clone
CAT#: RC203273
MAD2L1 (Myc-DDK-tagged)-Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1)
ORF Plasmid: tGFP
"NM_002358" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HSMAD2; MAD2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203273 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCAGCTCTCCCGGGAGCAGGGAATCACCCTGCGCGGGAGCGCCGAAATCGTGGCCGAGTTCT TCTCATTCGGCATCAACAGCATTTTATATCAGCGTGGCATATATCCATCTGAAACCTTTACTCGAGTGCA GAAATACGGACTCACCTTGCTTGTAACTACTGATCTTGAGCTCATAAAATACCTAAATAATGTGGTGGAA CAACTGAAAGATTGGTTATACAAGTGTTCAGTTCAGAAACTGGTTGTAGTTATCTCAAATATTGAAAGTG GTGAGGTCCTGGAAAGATGGCAGTTTGATATTGAGTGTGACAAGACTGCAAAAGATGACAGTGCACCCAG AGAAAAGTCTCAGAAAGCTATCCAGGATGAAATCCGTTCAGTGATCAGACAGATCACAGCTACGGTGACA TTTCTGCCACTGTTGGAAGTTTCTTGTTCATTTGATCTGCTGATTTATACAGACAAAGATTTGGTTGTAC CTGAAAAATGGGAAGAGTCGGGACCACAGTTTATTACCAATTCTGAGGAAGTCCGCCTTCGTTCATTTAC TACTACAATCCACAAAGTAAATAGCATGGTGGCCTACAAAATTCCTGTCAATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203273 protein sequence
Red=Cloning site Green=Tags(s) MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVE QLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVT FLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002358 |
ORF Size | 615 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002358.4 |
RefSeq Size | 1453 bp |
RefSeq ORF | 618 bp |
Locus ID | 4085 |
UniProt ID | Q13257 |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
MW | 23.5 kDa |
Gene Summary | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
The HuR CMLD-2 inhibitor exhibits antitumor effects via MAD2 downregulation in thyroid cancer cells
,Allegri, L;Baldan, F;Roy, S;Aubé, J;Russo, D;Filetti, S;Damante, G;,
Sci Rep
,PubMed ID 31089242
[MAD2]
|
Mad2 Overexpression Uncovers a Critical Role for TRIP13 in Mitotic Exit
,Marks, DH;Thomas, R;Chin, Y;Shah, R;Khoo, C;Benezra, R;,
Cell Rep
,PubMed ID 28564602
[MAD2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203273L1 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203273L2 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mGFP tagged |
CNY 5,890.00 |
|
RC203273L3 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203273L4 | Lenti ORF clone of Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mGFP tagged |
CNY 4,800.00 |
|
RG203273 | MAD2L1 (tGFP-tagged) - Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) |
CNY 4,000.00 |
|
SC124109 | MAD2L1 (untagged)-Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) |
CNY 2,400.00 |