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PBK (NM_018492) Human Tagged ORF Clone
CAT＃: RC203295
PBK (Myc-DDK-tagged)-Human PDZ binding kinase (PBK)
ORF Plasmid: tGFP
"NM_018492" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT84; HEL164; Nori-3; SPK; TOPK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203295 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGATCAGTAATTTCAAGACACCAAGCAAATTATCAGAAAAAAAGAAATCTGTATTATGTTCAA CTCCAACTATAAATATCCCGGCCTCTCCATTTATGCAGAAGCTTGGCTTTGGTACTGGGGTAAATGTGTA CCTAATGAAAAGATCTCCAAGAGGTTTGTCTCATTCTCCTTGGGCTGTAAAAAAGATTAATCCTATATGT AATGATCATTATCGAAGTGTGTATCAAAAGAGACTAATGGATGAAGCTAAGATTTTGAAAAGCCTTCATC ATCCAAACATTGTTGGTTATCGTGCTTTTACTGAAGCCAATGATGGCAGTCTGTGTCTTGCTATGGAATA TGGAGGTGAAAAGTCTCTAAATGACTTAATAGAAGAACGATATAAAGCCAGCCAAGATCCTTTTCCAGCA GCCATAATTTTAAAAGTTGCTTTGAATATGGCAAGAGGGTTAAAGTATCTGCACCAAGAAAAGAAACTGC TTCATGGAGACATAAAGTCTTCAAATGTTGTAATTAAAGGCGATTTTGAAACAATTAAAATCTGTGATGT AGGAGTCTCTCTACCACTGGATGAAAATATGACTGTGACTGACCCTGAGGCTTGTTACATTGGCACAGAG CCATGGAAACCCAAAGAAGCTGTGGAGGAGAATGGTGTTATTACTGACAAGGCAGACATATTTGCCTTTG GCCTTACTTTGTGGGAAATGATGACTTTATCGATTCCACACATTAATCTTTCAAATGATGATGATGATGA AGATAAAACTTTTGATGAAAGTGATTTTGATGATGAAGCATACTATGCAGCGTTGGGAACTAGGCCACCT ATTAATATGGAAGAACTGGATGAATCATACCAGAAAGTAATTGAACTCTTCTCTGTATGCACTAATGAAG ACCCTAAAGATCGTCCTTCTGCTGCACACATTGTTGAAGCTCTGGAAACAGATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203295 protein sequence
Red=Cloning site Green=Tags(s) MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPIC NDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPA AIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTE PWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPP INMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018492 |
| ORF Size | 966 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018492.4 |
| RefSeq Size | 2127 bp |
| RefSeq ORF | 969 bp |
| Locus ID | 55872 |
| UniProt ID | Q96KB5 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 36.1 kDa |
| Gene Summary | This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
4-Hydroxytamoxifen enhances sensitivity of estrogen receptor α-positive breast cancer to docetaxel in an estrogen and ZNF423 SNP-dependent fashion
,Wang, G;Qin, S;Zayas, J;Ingle, JN;Liu, M;Weinshilboum, RM;Shen, K;Wang, L;,
Breast Cancer Res. Treat.
,PubMed ID 30937657
[PBK]
|
|
PBK overexpression promotes metastasis of hepatocellular carcinoma via activating ETV4-uPAR signaling pathway
,Yang, QX;Zhong, S;He, L;Jia, XJ;Tang, H;Cheng, ST;Ren, JH;Yu, HB;Zhou, L;Zhou, HZ;Ren, F;Hu, ZW;Gong, R;Huang, AL;Chen, J;,
Cancer Lett.
,PubMed ID 30914208
[PBK]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203295L1 | Lenti ORF clone of Human PDZ binding kinase (PBK), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203295L2 | Lenti ORF clone of Human PDZ binding kinase (PBK), mGFP tagged |
CNY 5,890.00 |
|
| RC203295L3 | Lenti ORF clone of Human PDZ binding kinase (PBK), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203295L4 | Lenti ORF clone of Human PDZ binding kinase (PBK), mGFP tagged |
CNY 4,800.00 |
|
| RG203295 | PBK (tGFP-tagged) - Human PDZ binding kinase (PBK) |
CNY 4,000.00 |
|
| SC113461 | PBK (untagged)-Human PDZ binding kinase (PBK) |
CNY 2,400.00 |
|
| SC323480 | PBK (untagged)-Kinase deficient mutant (K64M) of Human PDZ binding kinase (PBK) |
CNY 2,400.00 |
