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KLC1 (NM_005552) Human Tagged ORF Clone
CAT＃: RC203467
KLC1 (Myc-DDK-tagged)-Human kinesin light chain 1 (KLC1), transcript variant 1
ORF Plasmid: tGFP
"NM_005552" in other vectors (6)
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CNY 4,168.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KLC; KNS2; KNS2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203467 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACAACATGTCCACAATGGTGTACATAAAGGAAGACAAGTTGGAGAAGCTTACACAGGATGAAA TTATTTCTAAGACAAAGCAAGTAATTCAGGGGCTGGAAGCTTTGAAGAATGAGCACAATTCCATTTTACA AAGTTTGCTGGAGACACTGAAGTGTTTGAAGAAAGATGATGAAAGTAATTTGGTGGAGGAGAAATCAAAC ATGATCCGGAAGTCACTGGAGATGTTGGAGCTCGGCCTGAGTGAGGCACAGGTTATGATGGCTTTGTCAA ATCACCTGAATGCTGTGGAGTCCGAGAAGCAGAAACTGCGTGCGCAGGTTCGTCGTCTGTGCCAGGAGAA TCAGTGGCTACGGGATGAACTGGCCAACACGCAGCAGAAACTGCAGAAGAGTGAGCAGTCTGTGGCTCAA CTGGAGGAGGAGAAGAAGCATCTGGAGTTTATGAATCAGCTAAAAAAATATGATGACGACATTTCCCCAT CCGAGGACAAAGACACTGATTCTACCAAAGAGCCTCTGGATGACCTTTTCCCCAATGATGAAGACGACCC AGGGCAAGGAATCCAGCAGCAGCACAGCAGTGCAGCCGCGGCTGCCCAGCAGGGCGGCTACGAGATCCCC GCGCGGCTGCGGACGCTCCACAACCTGGTGATCCAGTACGCCTCGCAGGGGCGCTACGAGGTAGCTGTGC CCCTCTGCAAGCAGGCCCTGGAGGACCTGGAGAAGACTTCAGGACACGACCACCCGGACGTGGCCACCAT GCTCAACATCCTGGCCTTGGTGTACAGGGATCAGAATAAATACAAAGATGCAGCTAACCTACTGAATGAT GCCTTGGCTATTCGTGAGAAAACTTTGGGCAAAGATCATCCTGCGGTGGCGGCGACTTTGAATAACCTTG CAGTCCTTTATGGTAAAAGAGGGAAGTACAAAGAAGCAGAGCCGTTGTGTAAAAGAGCTCTGGAAATCCG AGAAAAGGTTTTGGGGAAGGATCACCCCGATGTTGCCAAGCAGTTAAATAACTTGGCCTTACTGTGCCAG AACCAGGGCAAGTATGAAGAAGTAGAATATTATTATCAAAGAGCCCTCGAGATCTACCAGACAAAACTGG GACCTGATGACCCCAACGTGGCTAAGACGAAAAATAACCTGGCATCCTGCTATTTGAAACAAGGAAAGTT CAAGCAAGCAGAAACACTGTACAAAGAGATTCTCACTCGTGCACATGAAAGGGAGTTTGGTTCTGTAGAT GATGAAAATAAACCCATCTGGATGCATGCTGAAGAAAGAGAAGAATGCAAAGGAAAGCAAAAGGATGGGA CATCTTTTGGAGAGTATGGCGGCTGGTACAAAGCCTGCAAAGTTGATAGTCCAACTGTTACAACCACTCT AAAAAACCTTGGGGCACTTTACAGACGTCAAGGCAAATTTGAAGCTGCAGAAACGTTAGAAGAAGCTGCT ATGAGGTCTCGTAAACAGGGTCTTGACAATGTTCACAAACAGAGGGTGGCAGAAGTGCTCAATGACCCTG AGAACATGGAGAAGCGCAGGAGCCGTGAGAGCCTCAACGTGGACGTGGTCAAGTACGAGAGTGGCCCTGA CGGAGGGGAGGAAGTGAGTATGAGCGTAGAGTGGAACGGGATGAGGAAAATGAAGCTCGGGCTGGTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203467 protein sequence
Red=Cloning site Green=Tags(s) MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSN MIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQ LEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIP ARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLND ALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQ NQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVD DENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAA MRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGMRKMKLGLVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005552 |
| ORF Size | 1680 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005552.4, NP_005543.2 |
| RefSeq Size | 2624 bp |
| RefSeq ORF | 1683 bp |
| Locus ID | 3831 |
| UniProt ID | Q07866 |
| Domains | TPR |
| Protein Families | Druggable Genome |
| MW | 63.8 kDa |
| Gene Summary | Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203467L1 | Lenti ORF clone of Human kinesin light chain 1 (KLC1), transcript variant 1, Myc-DDK-tagged |
CNY 6,568.00 |
|
| RC203467L2 | Lenti ORF clone of Human kinesin light chain 1 (KLC1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203467L3 | Lenti ORF clone of Human kinesin light chain 1 (KLC1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203467L4 | Lenti ORF clone of Human kinesin light chain 1 (KLC1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203467 | KLC1 (tGFP-tagged) - Human kinesin light chain 1 (KLC1), transcript variant 1 |
CNY 5,768.00 |
|
| SC320281 | KLC1 (untagged)-Human kinesin light chain 1 (KLC1), transcript variant 1 |
CNY 4,176.00 |
