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ATIC (NM_004044) Human Tagged ORF Clone
CAT＃: RC203490
ATIC (Myc-DDK-tagged)-Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)
ORF Plasmid: tGFP
"NM_004044" in other vectors (6)
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CNY 4,408.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203490 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGGCCAGCTCGCCTTATTTAGTGTCTCTGACAAAACCGGCCTTGTGGAATTTGCAAGAAACC TGACCGCTCTTGGTTTGAATCTGGTCGCTTCCGGAGGGACTGCAAAAGCTCTCAGGGATGCTGGTCTGGC AGTCAGAGATGTCTCTGAGTTGACGGGATTTCCTGAAATGTTGGGGGGACGTGTGAAAACTTTGCATCCT GCAGTCCATGCTGGAATCCTAGCTCGTAATATTCCAGAAGATAATGCTGACATGGCCAGACTTGATTTCA ATCTTATAAGAGTTGTTGCCTGCAATCTCTATCCCTTTGTAAAGACAGTGGCTTCTCCAGGTGTAACTGT TGAGGAGGCTGTGGAGCAAATTGACATTGGTGGAGTAACCTTACTGAGAGCTGCAGCCAAAAACCACGCT CGAGTGACAGTGGTGTGTGAACCAGAGGACTATGTGGTGGTGTCCACGGAGATGCAGAGCTCCGAGAGTA AGGACACCTCCTTGGAGACTAGACGCCAGTTAGCCTTGAAGGCATTCACTCATACGGCACAATATGATGA AGCAATTTCAGATTATTTCAGGAAACAGTACAGCAAAGGCGTATCTCAGATGCCCTTGAGATATGGAATG AACCCACATCAGACCCCTGCCCAGCTGTACACACTGCAGCCCAAGCTTCCCATCACAGTTCTAAATGGAG CCCCTGGATTTATAAACTTGTGCGATGCTTTGAACGCCTGGCAGCTGGTGAAGGAACTCAAGGAGGCTTT AGGTATTCCAGCCGCTGCCTCTTTCAAACATGTCAGCCCAGCAGGTGCTGCTGTTGGAATTCCACTCAGT GAAGATGAGGCCAAAGTCTGCATGGTTTATGATCTCTATAAAACCCTCACACCCATCTCAGCGGCATATG CAAGAGCAAGAGGGGCTGATAGGATGTCTTCATTTGGTGATTTTGTTGCATTGTCCGATGTTTGTGATGT ACCAACTGCAAAAATTATTTCCAGAGAAGTATCTGATGGTATAATTGCCCCAGGATATGAAGAAGAAGCC TTGACAATACTTTCCAAAAAGAAAAATGGAAACTATTGTGTCCTTCAGATGGACCAATCTTACAAACCAG ATGAAAATGAAGTTCGAACTCTCTTTGGTCTTCATTTAAGCCAGAAGAGAAATAATGGTGTCGTCGACAA GTCATTATTTAGCAATGTTGTTACCAAAAATAAAGATTTGCCAGAGTCTGCCCTCCGAGACCTCATCGTA GCCACCATTGCTGTCAAGTACACTCAGTCTAACTCTGTGTGCTACGCCAAGAACGGGCAGGTTATCGGCA TTGGAGCAGGACAGCAGTCTCGTATACACTGCACTCGCCTTGCAGGAGATAAGGCAAACTATTGGTGGCT TAGACACCATCCACAAGTGCTTTCGATGAAGTTTAAAACAGGAGTGAAGAGAGCAGAAATCTCCAATGCC ATCGATCAATATGTGACTGGAACCATTGGCGAGGATGAAGATTTGATAAAGTGGAAGGCACTGTTTGAGG AAGTCCCTGAGTTACTCACTGAGGCAGAGAAGAAGGAATGGGTTGAGAAACTGACTGAAGTTTCTATCAG CTCTGATGCCTTCTTCCCTTTCCGAGATAACGTAGACAGAGCTAAAAGGAGTGGTGTGGCGTACATTGCG GCTCCCTCCGGTTCTGCTGCTGACAAAGTTGTGATTGAGGCCTGCGACGAACTGGGAATCATCCTCGCTC ATACGAACCTTCGGCTCTTCCACCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203490 protein sequence
Red=Cloning site Green=Tags(s) MAPGQLALFSVSDKTGLVEFARNLTALGLNLVASGGTAKALRDAGLAVRDVSELTGFPEMLGGRVKTLHP AVHAGILARNIPEDNADMARLDFNLIRVVACNLYPFVKTVASPGVTVEEAVEQIDIGGVTLLRAAAKNHA RVTVVCEPEDYVVVSTEMQSSESKDTSLETRRQLALKAFTHTAQYDEAISDYFRKQYSKGVSQMPLRYGM NPHQTPAQLYTLQPKLPITVLNGAPGFINLCDALNAWQLVKELKEALGIPAAASFKHVSPAGAAVGIPLS EDEAKVCMVYDLYKTLTPISAAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIIAPGYEEEA LTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGVVDKSLFSNVVTKNKDLPESALRDLIV ATIAVKYTQSNSVCYAKNGQVIGIGAGQQSRIHCTRLAGDKANYWWLRHHPQVLSMKFKTGVKRAEISNA IDQYVTGTIGEDEDLIKWKALFEEVPELLTEAEKKEWVEKLTEVSISSDAFFPFRDNVDRAKRSGVAYIA APSGSAADKVVIEACDELGIILAHTNLRLFHH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004044 |
| ORF Size | 1776 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004044.7 |
| RefSeq Size | 2094 bp |
| RefSeq ORF | 1779 bp |
| Locus ID | 471 |
| UniProt ID | P31939 |
| Domains | AICARFT_IMPCHas, MGS |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Metabolic pathways, One carbon pool by folate, Purine metabolism |
| MW | 64.6 kDa |
| Gene Summary | This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ATIC facilitates cell growth and migration by upregulating Myc expression in lung adenocarcinoma
,null,
Oncology Letters
,PubMed ID 35251351
[ATIC]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203490L1 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), Myc-DDK-tagged |
CNY 6,808.00 |
|
| RC203490L2 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), mGFP tagged |
CNY 6,460.00 |
|
| RC203490L3 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203490L4 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), mGFP tagged |
CNY 6,460.00 |
|
| RG203490 | ATIC (tGFP-tagged) - Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) |
CNY 6,008.00 |
|
| SC320282 | ATIC (untagged)-Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) |
CNY 4,416.00 |
