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UAP1 (NM_003115) Human Tagged ORF Clone
CAT＃: RC203494
UAP1 (Myc-DDK-tagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1)
ORF Plasmid: tGFP
"NM_003115" in other vectors (4)
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CNY 3,760.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGX; AGX1; AGX2; SPAG2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203494 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATTAATGACCTCAAACTCACGTTGTCCAAAGCTGGGCAAGAGCACCTACTACGTTTCTGGAATG AGCTTGAAGAAGCCCAACAGGTAGAACTTTATGCAGAGCTCCAGGCCATGAACTTTGAGGAGCTGAACTT CTTTTTCCAAAAGGCCATTGAAGGTTTTAACCAGTCTTCTTACCAAAAGAATGTGGATGCACGAATGGAA CCTGTGCCTCGAGAGGTATTAGGCAGTGCTACAAGGGATCAAGATCAGCTCCAGGCCTGGGAAAGTGAAG GACTTTTCCAGATTTCTCAGAATAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTCGG CGTTGCATATCCTAAGGGGATGTATGATGTTGGTTTGCCATCCCGTAAGACACTTTTTCAGATTCAAGCA GAGCGTATCCTGAAGCTACAGCAGGTTGCTGAAAAATATTATGGCAACAAATGCATTATTCCATGGTATA TAATGACCAGTGGCAGAACAATGGAATCTACAAAGGAGTTCTTCACCAAGCACAAGTACTTTGGTTTAAA AAAAGAGAATGTAATCTTTTTTCAGCAAGGAATGCTCCCCGCCATGAGTTTTGATGGGAAAATTATTTTG GAAGAGAAGAACAAAGTTTCTATGGCTCCAGATGGGAATGGTGGTCTTTATCGGGCACTTGCAGCCCAGA ATATTGTGGAGGATATGGAGCAAAGAGGCATTTGGAGCATTCATGTCTATTGTGTTGACAACATATTAGT AAAAGTGGCAGACCCACGGTTCATTGGATTTTGCATTCAGAAAGGAGCAGACTGTGGAGCAAAGGTGGTA GAGAAAACGAACCCTACAGAACCAGTTGGAGTGGTTTGCCGAGTGGATGGAGTTTACCAGGTGGTAGAAT ATAGTGAGATTTCCCTGGCAACAGCTCAAAAACGAAGCTCAGACGGACGACTGCTGTTCAATGCGGGGAA CATTGCCAACCATTTCTTCACTGTACCATTTCTGAGAGATGTTGTCAATGTTTATGAACCTCAGTTGCAG CACCATGTGGCTCAAAAGAAGATTCCTTATGTGGATACCCAAGGACAGTTAATTAAGCCAGACAAACCCA ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTATATGAAGT ATTGCGAGAAGATGAGTTTTCCCCACTAAAGAATGCTGATAGTCAGAATGGGAAAGACAACCCTACTACT GCAAGGCATGCTTTGATGTCCCTTCATCATTGCTGGGTCCTCAATGCAGGGGGCCATTTCATAGATGAAA ATGGCTCTCGCCTTCCAGCAATTCCCCGCTTGAAGGATGCCAATGATGTACCAATCCAATGTGAAATCTC TCCTCTTATCTCCTATGCTGGAGAAGGATTAGAAAGTTATGTGGCAGATAAAGAATTCCATGCACCTCTA ATCATCGATGAGAATGGAGTTCATGAGCTGGTGAAAAATGGTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203494 protein sequence
Red=Cloning site Green=Tags(s) MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSYQKNVDARME PVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQA ERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIIL EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQ HHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPL IIDENGVHELVKNGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003115 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003115.3, NP_003106.2 |
| RefSeq Size | 2344 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 6675 |
| UniProt ID | Q16222 |
| Domains | UDPGP |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
| MW | 57.1 kDa |
| Gene Summary | Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Reciprocal Regulation Between O-GlcNAcylation and β-Catenin Facilitates Cell Viability and Inhibits Apoptosis in Liver Cancer
,Gao, S;Miao, Y;Liu, Y;Liu, X;Fan, X;Lin, Y;Qian, P;Zhou, J;Dai, Y;Xia, L;Zhu, P;Zhu, J;,
DNA Cell Biol.
,PubMed ID 30762425
[UAP1]
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Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners
,Seok-Ho Yu, Michael Boyce, Amberlyn M. Wands, Michelle R. Bond, Carolyn R. Bertozzi, and Jennifer J. Kohler,
PNAS, Mar 2012; 109: 4834 - 4839.
[UAP1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203494L3 | Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203494L4 | Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1), mGFP tagged |
CNY 5,890.00 |
|
| RG203494 | UAP1 (tGFP-tagged) - Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1) |
CNY 5,360.00 |
|
| SC118166 | UAP1 (untagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1) |
CNY 3,768.00 |
