TSPAN1 (NM_005727) Human Tagged ORF Clone
CAT#: RC203549
TSPAN1 (Myc-DDK-tagged)-Human tetraspanin 1 (TSPAN1)
ORF Plasmid: tGFP
"NM_005727" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | NET1; TM4C; TM4SF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203549 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGCTTCAGCTTCATTAAGACCATGATGATCCTCTTCAATTTGCTCATCTTTCTGTGTGGTGCAG CCCTGTTGGCAGTGGGCATCTGGGTGTCAATCGATGGGGCATCCTTTCTGAAGATCTTCGGGCCACTGTC GTCCAGTGCCATGCAGTTTGTCAACGTGGGCTACTTCCTCATCGCAGCCGGCGTTGTGGTCTTTGCTCTT GGTTTCCTGGGCTGCTATGGTGCTAAGACTGAGAGCAAGTGTGCCCTCGTGACGTTCTTCTTCATCCTCC TCCTCATCTTCATTGCTGAGGTTGCAGCTGCTGTGGTCGCCTTGGTGTACACCACAATGGCTGAGCACTT CCTGACGTTGCTGGTAGTGCCTGCCATCAAGAAAGATTATGGTTCCCAGGAAGACTTCACTCAAGTGTGG AACACCACCATGAAAGGGCTCAAGTGCTGTGGCTTCACCAACTATACGGATTTTGAGGACTCACCCTACT TCAAAGAGAACAGTGCCTTTCCCCCATTCTGTTGCAATGACAACGTCACCAACACAGCCAATGAAACCTG CACCAAGCAAAAGGCTCACGACCAAAAAGTAGAGGGTTGCTTCAATCAGCTTTTGTATGACATCCGAACT AATGCAGTCACCGTGGGTGGTGTGGCAGCTGGAATTGGGGGCCTCGAGCTGGCTGCCATGATTGTGTCCA TGTATCTGTACTGCAATCTACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203549 protein sequence
Red=Cloning site Green=Tags(s) MQCFSFIKTMMILFNLLIFLCGAALLAVGIWVSIDGASFLKIFGPLSSSAMQFVNVGYFLIAAGVVVFAL GFLGCYGAKTESKCALVTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVVPAIKKDYGSQEDFTQVW NTTMKGLKCCGFTNYTDFEDSPYFKENSAFPPFCCNDNVTNTANETCTKQKAHDQKVEGCFNQLLYDIRT NAVTVGGVAAGIGGLELAAMIVSMYLYCNLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005727 |
ORF Size | 723 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005727.4 |
RefSeq Size | 1639 bp |
RefSeq ORF | 726 bp |
Locus ID | 10103 |
UniProt ID | O60635 |
Domains | transmembrane4 |
Protein Families | Transmembrane |
MW | 26.3 kDa |
Gene Summary | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Tetraspanin1 promotes NGF signaling by controlling TrkA receptor proteostasis
,Ferrero Restelli, F;Fontanet, PA;De Vincenti, AP;Falzone, TL;Ledda, F;Paratcha, G;,
Cell. Mol. Life Sci.
,PubMed ID 31440771
[TSPAN1]
|
Tspan-1 interacts with the thiamine transporter-1 in human intestinal epithelial cells and modulates its stability
,Svetlana M. Nabokina, Sundar Rajan Senthilkumar, and Hamid M. Said,
Am J Physiol Gastrointest Liver Physiol, Nov 2011; 301: G808 - G813.
[TSPAN1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203549L1 | Lenti ORF clone of Human tetraspanin 1 (TSPAN1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203549L2 | Lenti ORF clone of Human tetraspanin 1 (TSPAN1), mGFP tagged |
CNY 5,890.00 |
|
RC203549L3 | Lenti ORF clone of Human tetraspanin 1 (TSPAN1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203549L4 | Lenti ORF clone of Human tetraspanin 1 (TSPAN1), mGFP tagged |
CNY 4,800.00 |
|
RG203549 | TSPAN1 (tGFP-tagged) - Human tetraspanin 1 (TSPAN1) |
CNY 4,000.00 |
|
SC321265 | TSPAN1 (untagged)-Human tetraspanin 1 (TSPAN1) |
CNY 2,400.00 |