HNRNPA0 (NM_006805) Human Tagged ORF Clone

CAT#: RC203666

HNRNPA0 (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0)

ORF Plasmid: DDK tGFP



  "NM_006805" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


HNRNPA0 mouse monoclonal antibody,clone OTI8H8
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HNRPA0
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203666 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATTCTCAGTTGTGTAAGCTGTTCATCGGCGGCCTCAATGTGCAGACGAGTGAGTCGGGCCTGC
GCGGCCACTTTGAGGCCTTTGGGACTCTGACGGACTGCGTGGTGGTGGTGAATCCCCAGACCAAGCGCTC
CCGTTGCTTTGGCTTCGTGACCTACTCCAATGTGGAGGAGGCGGACGCCGCCATGGCCGCCTCGCCCCAT
GCCGTGGACGGCAACACTGTGGAGCTGAAGCGGGCGGTGTCCCGGGAGGATTCGGCGCGGCCCGGTGCCC
ACGCCAAGGTTAAGAAGCTCTTTGTCGGAGGCCTTAAAGGAGACGTGGCTGAGGGCGACCTGATCGAGCA
CTTCTCGCAGTTTGGCACCGTGGAAAAGGCCGAGATTATTGCCGACAAGCAGTCCGGCAAGAAGCGTGGA
TTCGGCTTCGTGTATTTCCAGAATCACGACGCGGCAGACAAGGCCGCGGTGGTCAAGTTCCATCCGATTC
AGGGCCATCGCGTGGAGGTGAAGAAAGCAGTCCCCAAGGAGGATATCTACTCCGGTGGGGGTGGAGGCGG
CTCCCGATCCTCCCGGGGCGGCCGAGGCGGCCGGGGGCGCGGCGGTGGTCGAGACCAGAACGGCCTTTCC
AAGGGCGGCGGCGGCGGTTACAACAGCTACGGTGGTTACGGCGGCGGCGGAGGCGGCGGCTACAATGCCT
ACGGAGGCGGCGGCGGCGGTTCGTCCTACGGTGGGAGCGACTACGGTAACGGCTTCGGCGGCTTCGGCAG
CTACAGCCAGCATCAGTCCTCCTATGGGCCCATGAAGAGCGGCGGCGGCGGCGGCGGTGGAGGCAGTAGC
TGGGGCGGTCGCAGTAATAGTGGACCTTACAGAGGCGGCTATGGCGGTGGGGGTGGCTATGGAGGCAGCT
CCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203666 protein sequence
Red=Cloning site Green=Tags(s)

MENSQLCKLFIGGLNVQTSESGLRGHFEAFGTLTDCVVVVNPQTKRSRCFGFVTYSNVEEADAAMAASPH
AVDGNTVELKRAVSREDSARPGAHAKVKKLFVGGLKGDVAEGDLIEHFSQFGTVEKAEIIADKQSGKKRG
FGFVYFQNHDAADKAAVVKFHPIQGHRVEVKKAVPKEDIYSGGGGGGSRSSRGGRGGRGRGGGRDQNGLS
KGGGGGYNSYGGYGGGGGGGYNAYGGGGGGSSYGGSDYGNGFGGFGSYSQHQSSYGPMKSGGGGGGGGSS
WGGRSNSGPYRGGYGGGGGYGGSSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006805
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006805.4
RefSeq Size 2983 bp
RefSeq ORF 918 bp
Locus ID 10949
UniProt ID Q13151
Domains RRM
MW 30.8 kDa
Gene Summary This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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