UCHL5IP (HAUS7) (NM_017518) Human Tagged ORF Clone

CAT#: RC203737

HAUS7 (Myc-DDK-tagged)-Human HAUS augmin-like complex, subunit 7 (HAUS7)

ORF Plasmid: DDK tGFP



  "NM_017518" in other vectors (5)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


HAUS7 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms UCHL5IP; UIP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203737 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGGGGCGCGGCTCGGAGCGCGAAACATGGCGGGGCAGGACGCTGGCTGCGGCCGTGGCGGCGACG
ACTACTCAGAGGACGAGGGCGACAGCAGCGTGTCCAGGGCGGCTGTGGAGGTGTTCGGGAAGCTGAAGGA
CCTAAACTGCCCCTTCCTCGAGGGTCTGTATATCACAGAGCCAAAGACAATTCAGGAACTGCTGTGCAGC
CCCTCAGAGTACCGCTTGGAGATCCTAGAGTGGATGTGTACCCGGGTCTGGCCCTCACTGCAGGACAGGT
TCAGCTCACTGAAAGGGGTCCCAACAGAGGTGAAGATCCAAGAAATGACGAAGCTGGGCCACGAGCTGAT
GCTGTGTGCGCCAGATGACCAGGAGCTCCTCAAGGGCTGTGCCTGCGCCCAGAAGCAGCTACACTTCATG
GACCAGTTGCTCGATACCATCCGGAGCCTGACCATTGGGTGCTCCAGTTGCTCGAGCCTGATGGAGCACT
TCGAGGACACCAGGGAGAAGAACGAGGCCTTGCTGGGGGAGCTCTTCTCTAGCCCCCACCTGCAGATGCT
CCTGAATCCAGAGTGCGACCCGTGGCCCCTGGACATGCAGCCCCTCCTCAACAAGCAGAGTGATGACTGG
CAGTGGGCCAGTGCCTCTGCCAAGTCCGAGGAGGAGGAGAAGCTGGCGGAGCTTGCCAGGCAGCTGCAGG
AGAGTGCTGCCAAGTTGCACGCGCTTAGAACGGAGTACTTTGCACAGCATGAGCAAGGGGCTGCTGCGGG
CGCAGCCGACATCAGCACCCTAGACCAGAAGCTGCGTCTGGTCACTTCCGACTTCCACCAGCTAATCTTG
GCTTTTCTCCAAGTCTACGACGACGAGCTGGGCGAGTGCTGCCAGCGCCCAGGCCCTGACCTCCACCCGT
GCGGCCCCATCATCCAGGCCACGCACCAGAATCTGACTTCCTACAGCCAACTGCTGCAAGTGGTCATGGC
AGTTGCTGACACCTCTGCGAAGGCCGTGGAGACCGTGAAGAAGCAGCAAGGCGAGCAGATCTGCTGGGGT
GGCAGCAGCTCCGTCATGAGTCTAGCTACCAAGATGAATGAACTAATGGAGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203737 protein sequence
Red=Cloning site Green=Tags(s)

MGGARLGARNMAGQDAGCGRGGDDYSEDEGDSSVSRAAVEVFGKLKDLNCPFLEGLYITEPKTIQELLCS
PSEYRLEILEWMCTRVWPSLQDRFSSLKGVPTEVKIQEMTKLGHELMLCAPDDQELLKGCACAQKQLHFM
DQLLDTIRSLTIGCSSCSSLMEHFEDTREKNEALLGELFSSPHLQMLLNPECDPWPLDMQPLLNKQSDDW
QWASASAKSEEEEKLAELARQLQESAAKLHALRTEYFAQHEQGAAAGAADISTLDQKLRLVTSDFHQLIL
AFLQVYDDELGECCQRPGPDLHPCGPIIQATHQNLTSYSQLLQVVMAVADTSAKAVETVKKQQGEQICWG
GSSSVMSLATKMNELMEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017518
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017518.7
RefSeq Size 1378 bp
RefSeq ORF 1107 bp
Locus ID 55559
UniProt ID Q99871
MW 40.8 kDa
Gene Summary This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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