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CHI3L1 (NM_001276) Human Tagged ORF Clone
CAT＃: RC203769
CHI3L1 (Myc-DDK-tagged)-Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1)
ORF Plasmid: tGFP
"NM_001276" in other vectors (7)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; hCGP-39; YK-40; YKL-40; YKL40; YYL-40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203769 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTGAAGGCGTCTCAAACAGGCTTTGTGGTCCTGGTGCTGCTCCAGTGCTGCTCTGCATACAAAC TGGTCTGCTACTACACCAGCTGGTCCCAGTACCGGGAAGGCGATGGGAGCTGCTTCCCAGATGCCCTTGA CCGCTTCCTCTGTACCCACATCATCTACAGCTTTGCCAATATAAGCAACGATCACATCGACACCTGGGAG TGGAATGATGTGACGCTCTACGGCATGCTCAACACACTCAAGAACAGGAACCCCAACCTGAAGACTCTCT TGTCTGTCGGAGGATGGAACTTTGGGTCTCAAAGATTTTCCAAGATAGCCTCCAACACCCAGAGTCGCCG GACTTTCATCAAGTCAGTACCGCCATTTCTGCGCACCCATGGCTTTGATGGGCTGGACCTTGCCTGGCTC TACCCTGGACGGAGAGACAAACAGCATTTTACCACCCTAATCAAGGAAATGAAGGCCGAATTTATAAAGG AAGCCCAGCCAGGGAAAAAGCAGCTCCTGCTCAGCGCAGCACTGTCTGCGGGGAAGGTCACCATTGACAG CAGCTATGACATTGCCAAGATATCCCAACACCTGGATTTCATTAGCATCATGACCTACGATTTTCATGGA GCCTGGCGTGGGACCACAGGCCATCACAGTCCCCTGTTCCGAGGTCAGGAGGATGCAAGTCCTGACAGAT TCAGCAACACTGACTATGCTGTGGGGTACATGTTGAGGCTGGGGGCTCCTGCCAGTAAGCTGGTGATGGG CATCCCCACCTTCGGGAGGAGCTTCACTCTGGCTTCTTCTGAGACTGGTGTTGGAGCCCCAATCTCAGGA CCGGGAATTCCAGGCCGGTTCACCAAGGAGGCAGGGACCCTTGCCTACTATGAGATCTGTGACTTCCTCC GCGGAGCCACAGTCCATAGAATCCTCGGCCAGCAGGTCCCCTATGCCACCAAGGGCAACCAGTGGGTAGG ATACGACGACCAGGAAAGCGTCAAAAGCAAGGTGCAGTACCTGAAGGACAGGCAGCTGGCGGGCGCCATG GTATGGGCCCTGGACCTGGATGACTTCCAGGGCTCCTTCTGCGGCCAGGATCTGCGCTTCCCTCTCACCA ATGCCATCAAGGATGCACTCGCTGCAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203769 protein sequence
Red=Cloning site Green=Tags(s) MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWE WNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWL YPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHG AWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISG PGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAM VWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001276 |
| ORF Size | 1149 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001276.3 |
| RefSeq Size | 1867 bp |
| RefSeq ORF | 1152 bp |
| Locus ID | 1116 |
| UniProt ID | P36222 |
| Domains | Glyco_18 |
| Protein Families | Secreted Protein |
| MW | 42.6 kDa |
| Gene Summary | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chitinase 3 like 1 (CHI3L1) is a regulator of smooth muscle cell physiology and atherosclerotic lesion stability
,Tsantilas, P;Lao, S;Wu, Z;Eberhard, A;Winski, G;Vaerst, M;Nanda, V;Wang, Y;Kojima, Y;Ye, J;Flores, A;Jarr, KU;Pelisek, J;Eckstein, HH;Matic, L;Hedin, U;Tsao, PS;Paloschi, V;Maegdefessel, L;Leeper, NJ;,
Cardiovascular research
,PubMed ID 33471078
[CHI3L1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203769L1 | Lenti ORF clone of Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203769L2 | Lenti ORF clone of Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), mGFP tagged |
CNY 7,888.00 |
|
| RC203769L3 | Lenti ORF clone of Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203769L4 | Lenti ORF clone of Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), mGFP tagged |
CNY 5,890.00 |
|
| RG203769 | CHI3L1 (tGFP-tagged) - Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) |
CNY 7,088.00 |
|
| SC125328 | CHI3L1 (untagged)-Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) |
CNY 5,488.00 |
|
| SC324578 | CHI3L1 (untagged)-Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) |
CNY 5,488.00 |
