STAR (NM_000349) Human Tagged ORF Clone
CAT#: RC203803
STAR (Myc-DDK-tagged)-Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_000349" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | STARD1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203803 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTAGCGACATTCAAGCTGTGCGCTGGGAGCTCCTACAGACACATGCGCAACATGAAGGGGCTGA GGCAACAGGCTGTGATGGCCATCAGCCAGGAGCTGAACCGGAGGGCCCTGGGGGGCCCCACCCCTAGCAC GTGGATTAACCAGGTTCGGCGGCGGAGCTCTCTACTCGGTTCTCGGCTGGAAGAGACTCTCTACAGTGAC CAGGAGCTGGCCTATCTCCAGCAGGGGGAGGAGGCCATGCAGAAGGCCTTGGGCATCCTTAGCAACCAAG AGGGCTGGAAGAAGGAGAGTCAGCAGGACAATGGGGACAAAGTGATGAGTAAAGTGGTCCCAGATGTGGG CAAGGTGTTCCGGCTGGAGGTCGTGGTGGACCAGCCCATGGAGAGGCTCTATGAAGAGCTCGTGGAGCGC ATGGAAGCAATGGGGGAGTGGAACCCCAATGTCAAGGAGATCAAGGTCCTGCAGAAGATCGGAAAAGATA CATTCATTACTCACGAGCTGGCTGCCGAGGCAGCAGGAAACCTGGTGGGGCCCCGTGACTTTGTGAGCGT GCGCTGTGCCAAGCGCCGAGGCTCCACCTGTGTGCTGGCTGGCATGGCCACAGACTTCGGGAACATGCCT GAGCAGAAGGGTGTCATCAGGGCGGAGCACGGTCCCACTTGCATGGTGCTTCACCCGTTGGCTGGAAGTC CCTCTAAGACCAAACTTACGTGGCTACTCAGCATCGACCTCAAGGGGTGGCTGCCCAAGAGCATCATCAA CCAGGTCCTGTCCCAGACCCAGGTGGATTTTGCCAACCACCTGCGCAAGCGCCTGGAGTCCCACCCTGCC TCTGAAGCCAGGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203803 protein sequence
Red=Cloning site Green=Tags(s) MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSD QELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVER MEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMP EQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPA SEARC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000349 |
ORF Size | 855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_000349.3 |
RefSeq Size | 2695 bp |
RefSeq ORF | 858 bp |
Locus ID | 6770 |
UniProt ID | P49675 |
Protein Families | Druggable Genome |
MW | 31.9 kDa |
Gene Summary | The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203803L1 | Lenti ORF clone of Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203803L2 | Lenti ORF clone of Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RC203803L3 | Lenti ORF clone of Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203803L4 | Lenti ORF clone of Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG203803 | STAR (tGFP-tagged) - Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein |
CNY 4,000.00 |
|
SC300053 | STAR (untagged)-Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
SC322286 | STAR (untagged)-Human steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |