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KIF12 (NM_138424) Human Tagged ORF Clone
CAT＃: RC203891
KIF12 (Myc-DDK-tagged)-Human kinesin family member 12 (KIF12)
ORF Plasmid: tGFP
"NM_138424" in other vectors (4)
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CNY 3,824.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203891 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGGACCTTCGCCTGGCTGTTGGACCGCGTGCAGCACCTGGGTGCCCCTGTCACCCTTCGCGCCT CTTATCTGGAGATCTACAATGAGCAGGTTCGGGACTTGCTGAGCCTGGGGTCTCCCCGGCCCCTCCCTGT TCGCTGGAACAAGACTCGGGGCTTCTATGTGGAGCAGCTGCGGGTGGTGGAATTTGGGAGTCTGGAGGCC CTGATGGAACTTTTGCAAACGGGTCTCAGCCGTCGAAGGAACTCAGCCCACACCCTGAACCAGGCCTCCA GCCGAAGCCATGCCCTGCTCACCCTTTACATCAGCCGTCAAACTGCCCAGCAGATGCCTTCTGTGGACCC TGGGGAGCCCCCTGTTGGTGGGAAGCTGTGCTTTGTGGACCTGGCAGGCAGTGAGAAGGTAGCAGCCACG GGATCCCGTGGGGAGCTGATGCTTGAGGCTAACAGCATCAACCGAAGCCTGCTGGCCCTGGGTCACTGCA TCTCCCTGCTGCTGGACCCACAGCGGAAGCAGAGCCACATCCCTTTCCGGGACAGCAAGCTCACCAAGTT GCTGGCAGACTCACTGGGAGGGCGCGGGGTCACCCTCATGGTGGCCTGCGTGTCCCCCTCAGCCCAGTGC CTTCCTGAGACTCTCAGCACCCTGCGATATGCAAGCCGAGCTCAGCGGGTCACCACCCGACCACAGGCCC CCAAGTCTCCTGTGGCAAAGCAGCCCCAGCGTTTGGAGACAGAGATGCTGCAGCTCCAGGAGGAGAACCG TCGCCTGCAGTTCCAGCTGGACCAAATGGACTGCAAGGCCTCAGGGCTCAGTGGAGCCCGGGTGGCCTGG GCCCAGCGGAACCTGTACGGGATGCTACAGGAGTTCATGCTAGAGAATGAGAGGCTCAGGAAAGAAAAGA GCCAGCTGCAGAATAGCCGAGACCTGGCCCAGAATGAGCAGCGCATCCTGGCCCAGCAGGTCCATGCACT AGAGAGGCGTCTCCTCTCTGCCTGCTACCATCACCAGCAGGGTCCTGGCCTGACCCCACCGTGTCCCTGC TTGATGGCCCCAGCTCCCCCTTGCCATGCACTGCCACCCCTCTACTCCTGCCCCTGCTGCCACATCTGCC CACTGTGTCGAGTGCCCCTGGCCCACTGGGCCTGCCTGCCAGGGGAGCACCACCTGCCCCAGGTGTTGGA CCCTGAGGCCTCAGGTGGCAGGCCCCCATCTGCCCGGCCCCCACCCTGGGCACCCCCATGCAGCCCTGGC TCTGCCAAGTGCCCAAGAGAGAGGAGTCACAGTGACTGGACTCAGACCCGAGTCCTGGCAGAGATGTTGA CGGAGGAGGAGGTGGTACCTTCTGCACCTCCCCTGCCTGTGAGGCCCCCGAAGACATCACCAGGGCTCAG AGGTGGGGCCGGGGTTCCAAACCTGGCCCAGAGACTGGAGGCCCTCAGAGACCAGATTGGCAGCTCCCTG CGACGTGGCCGCAGCCAGCCACCCTGCAGTGAGGGCGCACGGAGCCCAGGCCAAGTCCTCCCTCCCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203891 protein sequence
Red=Cloning site Green=Tags(s) MQRTFAWLLDRVQHLGAPVTLRASYLEIYNEQVRDLLSLGSPRPLPVRWNKTRGFYVEQLRVVEFGSLEA LMELLQTGLSRRRNSAHTLNQASSRSHALLTLYISRQTAQQMPSVDPGEPPVGGKLCFVDLAGSEKVAAT GSRGELMLEANSINRSLLALGHCISLLLDPQRKQSHIPFRDSKLTKLLADSLGGRGVTLMVACVSPSAQC LPETLSTLRYASRAQRVTTRPQAPKSPVAKQPQRLETEMLQLQEENRRLQFQLDQMDCKASGLSGARVAW AQRNLYGMLQEFMLENERLRKEKSQLQNSRDLAQNEQRILAQQVHALERRLLSACYHHQQGPGLTPPCPC LMAPAPPCHALPPLYSCPCCHICPLCRVPLAHWACLPGEHHLPQVLDPEASGGRPPSARPPPWAPPCSPG SAKCPRERSHSDWTQTRVLAEMLTEEEVVPSAPPLPVRPPKTSPGLRGGAGVPNLAQRLEALRDQIGSSL RRGRSQPPCSEGARSPGQVLPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138424 |
| ORF Size | 1539 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138424.1, NP_612433.1 |
| RefSeq Size | 2024 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 113220 |
| UniProt ID | Q96FN5 |
| Protein Families | Druggable Genome |
| MW | 56.6 kDa |
| Gene Summary | This gene encodes a member of the kinesin superfamily of microtubule-associated molecular motors with functions related to the microtubule cytosekelton. Members of this superfamily play important roles in intracellular transport and cell division. A similar protein in mouse functions in the beta cell antioxidant signaling cascade, acting as a scaffold for the transcription factor specificity protein 1 (Sp1). Mice that lack this gene exhibit beta cell oxidative stress resulting in hypoinsulinemic glucose intolerance. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203891L3 | Lenti ORF clone of Human kinesin family member 12 (KIF12), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203891L4 | Lenti ORF clone of Human kinesin family member 12 (KIF12), mGFP tagged |
CNY 6,460.00 |
|
| RG203891 | KIF12 (tGFP-tagged) - Human kinesin family member 12 (KIF12) |
CNY 5,424.00 |
|
| SC123136 | KIF12 (untagged)-Human kinesin family member 12 (KIF12) |
CNY 3,832.00 |
