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RNF86 (TRIM2) (NM_015271) Human Tagged ORF Clone
CAT＃: RC203947
- TrueORF®
TRIM2 (Myc-DDK-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1
ORF Plasmid: tGFP
"NM_015271" in other vectors (5)
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CNY 8,176.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMT2R; RNF86 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203947 representing NM_015271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGAAGGCACCAACATCCCAAGTCCTGTGGTGCGCCAGATTGACAAGCAGTTTCTGATTTGCA GTATATGCCTGGAACGGTACAAGAATCCCAAGGTTCTCCCCTGTCTGCACACTTTCTGCGAGAGGTGCCT GCAGAACTACATTCCTGCCCACAGTTTAACCCTCTCCTGCCCAGTGTGCCGCCAGACCTCCATCCTGCCC GAGAAAGGGGTGGCCGCGCTCCAGAACAATTTCTTCATCACAAACCTGATGGACGTGCTGCAGCGAACTC CAGGCAGCAACGCTGAGGAGTCTTCCATCCTGGAGACAGTCACTGCTGTGGCTGCGGGAAAGCCTCTCTC TTGCCCAAACCACGATGGGAATGTGATGGAATTTTACTGCCAGTCCTGTGAGACTGCCATGTGTCGGGAG TGCACGGAGGGGGAGCACGCAGAGCACCCCACAGTTCCACTCAAGGATGTGGTGGAACAGCACAAGGCCT CGCTCCAGGTCCAGCTGGATGCTGTCAACAAAAGGCTCCCAGAAATAGATTCTGCTCTTCAGTTCATCTC TGAAATCATTCATCAGTTAACCAACCAAAAGGCCAGCATCGTGGATGACATTCATTCCACCTTTGATGAG CTCCAGAAGACTTTAAATGTGCGCAAGAGTGTGCTGCTTATGGAATTGGAGGTCAACTATGGCCTCAAAC ACAAAGTCCTCCAGTCGCAGCTGGATACTCTGCTCCAGGGGCAGGAGAGCATTAAGAGCTGCAGCAACTT CACAGCGCAGGCCCTCAACCATGGCACGGAGACCGAGGTCCTACTGGTGAAGAAGCAGATGAGCGAGAAG CTGAACGAGCTGGCCGACCAGGACTTCCCCTTGCACCCGCGGGAGAACGACCAGCTGGATTTCATCGTGG AAACCGAGGGGCTGAAGAAGTCCATCCACAACCTCGGGACGATCTTAACCACCAACGCCGTTGCCTCAGA GACAGTGGCCACGGGCGAGGGGCTGCGGCAGACCATCATCGGGCAGCCCATGTCCGTCACCATCACCACC AAGGACAAAGACGGTGAGCTGTGCAAAACCGGCAACGCCTACCTCACCGCCGAACTGAGCACCCCCGACG GGAGCGTGGCAGACGGGGAGATCCTGGACAACAAGAACGGCACCTATGAGTTTTTGTACACTGTCCAGAA GGAAGGGGACTTTACCCTGTCTCTGAGACTCTATGACCAGCACATCCGAGGCAGCCCGTTTAAGCTGAAA GTGATCCGATCCGCTGATGTGTCTCCCACCACAGAAGGCGTGAAGAGGCGCGTTAAGTCCCCGGGGAGCG GCCACGTCAAGCAGAAAGCTGTGAAAAGACCCGCAAGCATGTACAGCACTGGAAAACGAAAAGAGAATCC CATCGAAGACGATTTGATCTTTCGAGTGGGTACCAAAGGAAGAAATAAAGGAGAGTTTACAAATCTTCAG GGGGTAGCTGCATCTACAAATGGAAAGATATTAATTGCAGACAGTAACAACCAATGTGTGCAGATATTTT CCAATGATGGCCAGTTCAAAAGTCGTTTTGGCATACGGGGACGCTCTCCGGGGCAGCTGCAGCGGCCCAC AGGAGTGGCTGTACATCCCAGTGGGGACATAATCATTGCCGATTATGATAATAAATGGGTCAGCATTTTC TCCTCCGATGGGAAATTTAAGACAAAAATTGGATCAGGAAAGCTGATGGGACCCAAAGGAGTTTCTGTGG ACCGCAATGGGCACATTATTGTTGTGGACAACAAGGCGTGCTGCGTGTTTATCTTCCAGCCAAACGGGAA AATAGTCACCAGGTTTGGTAGCCGAGGAAATGGGGACAGGCAGTTTGCAGGTCCCCATTTTGCAGCTGTA AATAGCAATAATGAGATTATTATTACAGATTTCCATAATCATTCTGTCAAGGTGTTTAATCAGGAAGGAG AATTCATGTTGAAGTTTGGCTCAAATGGAGAAGGAAATGGGCAGTTTAATGCTCCAACAGGTGTAGCAGT GGATTCAAATGGAAACATCATTGTGGCCGACTGGGGAAACAGCAGGATCCAGGTTTTTGATGGGAGTGGA TCATTTTTGTCCTACATTAACACATCTGCTGACCCACTCTATGGCCCCCAAGGCCTGGCCCTAACTTCAG ATGGTCATGTTGTGGTTGCAGACTCTGGAAATCACTGTTTCAAAGTCTATCGATACTTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203947 representing NM_015271
Red=Cloning site Green=Tags(s) MASEGTNIPSPVVRQIDKQFLICSICLERYKNPKVLPCLHTFCERCLQNYIPAHSLTLSCPVCRQTSILP EKGVAALQNNFFITNLMDVLQRTPGSNAEESSILETVTAVAAGKPLSCPNHDGNVMEFYCQSCETAMCRE CTEGEHAEHPTVPLKDVVEQHKASLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDE LQKTLNVRKSVLLMELEVNYGLKHKVLQSQLDTLLQGQESIKSCSNFTAQALNHGTETEVLLVKKQMSEK LNELADQDFPLHPRENDQLDFIVETEGLKKSIHNLGTILTTNAVASETVATGEGLRQTIIGQPMSVTITT KDKDGELCKTGNAYLTAELSTPDGSVADGEILDNKNGTYEFLYTVQKEGDFTLSLRLYDQHIRGSPFKLK VIRSADVSPTTEGVKRRVKSPGSGHVKQKAVKRPASMYSTGKRKENPIEDDLIFRVGTKGRNKGEFTNLQ GVAASTNGKILIADSNNQCVQIFSNDGQFKSRFGIRGRSPGQLQRPTGVAVHPSGDIIIADYDNKWVSIF SSDGKFKTKIGSGKLMGPKGVSVDRNGHIIVVDNKACCVFIFQPNGKIVTRFGSRGNGDRQFAGPHFAAV NSNNEIIITDFHNHSVKVFNQEGEFMLKFGSNGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDGSG SFLSYINTSADPLYGPQGLALTSDGHVVVADSGNHCFKVYRYLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015271 |
| ORF Size | 2232 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015271.2, NP_056086.1 |
| RefSeq Size | 6734 bp |
| RefSeq ORF | 2316 bp |
| Locus ID | 23321 |
| UniProt ID | Q9C040 |
| Domains | zf-B_box, NHL, Filamin, RING, BBC |
| MW | 81.3 kDa |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203947L3 | Lenti-ORF clone of TRIM2 (Myc-DDK-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1 |
CNY 7,700.00 |
|
| RC203947L4 | Lenti-ORF clone of TRIM2 (mGFP-tagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1 |
CNY 7,700.00 |
|
| RG203947 | TRIM2 (tGFP-tagged) - Human tripartite motif containing 2 (TRIM2), transcript variant 1 |
CNY 6,370.00 |
|
| SC112638 | TRIM2 (untagged)-Human tripartite motif containing 2 (TRIM2), transcript variant 1 |
CNY 5,456.00 |
|
| SC328003 | TRIM2 (untagged)-Human tripartite motif-containing 2 (TRIM2) transcript variant 1 |
CNY 14,920.00 |
