Ubiquilin (UBQLN1) (NM_053067) Human Tagged ORF Clone

CAT#: RC204020

UBQLN1 (Myc-DDK-tagged)-Human ubiquilin 1 (UBQLN1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_053067" in other vectors (5)

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CNY 4,176.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-UBQLN1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DA41; DSK2; PLIC-1; UBQN; XDRP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204020 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGATAGCGCCGCCGGAGCCGAAGGTGCTG
GCGCCCCCGCGGCCGCTGCCTCCGCGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAA
GGAGGAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAATCTCTAAACGTTTTAAA
TCACATACTGACCAACTTGTGTTGATATTTGCTGGAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGC
ATGGAATTCATGATGGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCATTCAGC
TCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAACTCCTAATAGTAACTCTACATCTGGT
TCTGCTACTAGCAACCCTTTTGGTTTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGA
ATACTACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTAACCCTGAAATGATGGT
CCAGATCATGGAAAATCCCTTTGTTCAGAGCATGCTCTCAAATCCTGACCTGATGAGACAGTTAATTATG
GCCAATCCACAAATGCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATCCAGATA
TAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATGCAGGAGATGATGAGGAACCAGGACCG
AGCTTTGAGCAACCTAGAAAGCATCCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAG
GAACCAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCCTTGGTGAGCAATACAT
CCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGAAAATAGAGATCCACTACCCAATCCATGGGCTCCACA
GACTTCCCAGAGTTCATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGTACTGCC
AGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCCTGGAGTAGGAGCTAGTATGTTCAACA
CACCAGGAATGCAGAGCTTGTTGCAACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGC
CCCCTACATGAGAAGCATGATGCAGTCACTAAGCCAGAATCCTGACCTTGCTGCACAGATGCAGAATCCT
GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACAGATTCAGCAGGGTTTACAGA
CATTAGCAACGGAAGCCCCGGGCCTCATCCCAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGG
AGGCTCTTCGGGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGCAGGAACCACT
GAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGGCTCTTGCTGGAGTAAATCCTCAGCTACAGA
ATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAA
CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCA
TCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204020 protein sequence
Red=Cloning site Green=Tags(s)

MAESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFK
SHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSG
SATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIM
ANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQ
EPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTA
SGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMQNP
DTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTT
EPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQP
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053067
ORF Size 1683 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053067.3
RefSeq Size 4093 bp
RefSeq ORF 1686 bp
Locus ID 29979
UniProt ID Q9UMX0
Domains UBA, UBQ, STI1
Protein Families Druggable Genome
MW 59.2 kDa
Gene Summary This gene encodes an ubiquitin-like protein (ubiquilin) that shares a high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and it is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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