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TM7SF1 (GPR137B) (NM_003272) Human Tagged ORF Clone
CAT＃: RC204155
GPR137B (Myc-DDK-tagged)-Human G protein-coupled receptor 137B (GPR137B)
ORF Plasmid: tGFP
"NM_003272" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TM7SF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204155 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCCGAGCGTCCCCGGCCGCGCGGCAGCGCCCCCGGCCCGATGGAGACCCCGCCGTGGGACCCAG CCCGCAACGACTCGCTGCCGCCCACGCTGACCCCGGCCGTGCCCCCCTACGTGAAGCTTGGCCTCACCGT CGTCTACACCGTGTTCTACGCGCTGCTCTTCGTGTTCATCTACGTGCAGCTCTGGCTGGTGCTGCGTTAC CGCCACAAGCGGCTCAGCTACCAGAGCGTCTTCCTCTTTCTCTGCCTCTTCTGGGCCTCCCTGCGGACCG TCCTCTTCTCCTTCTACTTCAAAGACTTCGTGGCGGCCAATTCGCTCAGCCCCTTCGTCTTCTGGCTGCT CTACTGCTTCCCTGTGTGCCTGCAGTTTTTCACCCTCACGCTGATGAACTTGTACTTCACGCAGGTGATT TTCAAAGCCAAGTCAAAATATTCTCCAGAATTACTCAAATACCGGTTGCCCCTCTACCTGGCCTCCCTCT TCATCAGCCTTGTTTTCCTGTTGGTGAATTTAACCTGTGCTGTGCTGGTAAAGACGGGAAATTGGGAGAG GAAGGTTATCGTCTCTGTGCGAGTGGCCATTAATGACACGCTCTTCGTGCTGTGTGCCGTCTCTCTCTCC ATCTGTCTCTACAAAATCTCTAAGATGTCCTTAGCCAACATTTACTTGGAGTCCAAGGGCTCCTCCGTGT GTCAAGTGACTGCCATCGGTGTCACCGTGATACTGCTTTACACCTCTCGGGCCTGCTACAACCTGTTCAT CCTGTCATTTTCTCAGAACAAGAGCGTCCATTCCTTTGATTATGACTGGTACAATGTATCAGACCAGGCA GATTTGAAGAATCAGCTGGGAGATGCTGGATACGTATTATTTGGAGTGGTGTTATTTGTTTGGGAACTCT TACCTACCACCTTAGTCGTTTATTTCTTCCGAGTTAGAAATCCTACAAAGGACCTTACCAACCCTGGAAT GGTCCCCAGCCATGGATTCAGTCCCAGATCTTATTTCTTTGACAACCCTCGAAGATATGACAGTGATGAT GACCTTGCCTGGAACATTGCCCCTCAGGGACTTCAGGGAGGTTTTGCTCCAGATTACTATGATTGGGGAC AACAAACTAACAGCTTCCTGGCACAAGCAGGAACTTTGCAAGACTCAACTTTGGATCCTGACAAACCAAG CCTTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204155 protein sequence
Red=Cloning site Green=Tags(s) MRPERPRPRGSAPGPMETPPWDPARNDSLPPTLTPAVPPYVKLGLTVVYTVFYALLFVFIYVQLWLVLRY RHKRLSYQSVFLFLCLFWASLRTVLFSFYFKDFVAANSLSPFVFWLLYCFPVCLQFFTLTLMNLYFTQVI FKAKSKYSPELLKYRLPLYLASLFISLVFLLVNLTCAVLVKTGNWERKVIVSVRVAINDTLFVLCAVSLS ICLYKISKMSLANIYLESKGSSVCQVTAIGVTVILLYTSRACYNLFILSFSQNKSVHSFDYDWYNVSDQA DLKNQLGDAGYVLFGVVLFVWELLPTTLVVYFFRVRNPTKDLTNPGMVPSHGFSPRSYFFDNPRRYDSDD DLAWNIAPQGLQGGFAPDYYDWGQQTNSFLAQAGTLQDSTLDPDKPSLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003272 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003272.4 |
| RefSeq Size | 2046 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 7107 |
| UniProt ID | O60478 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 45.6 kDa |
| Gene Summary | Lysosomal integral membrane protein that regulates the localization and activity of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:31036939). Interacts with Rag GTPases and increases the lysosomial localization and activity of Rag GTPases and thereby regulates mTORC1 translocation and activity in lysosome (PubMed:31036939). Involved in the regulation of lysosomal morphology and autophagy (PubMed:31036939).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204155L3 | Lenti ORF clone of Human G protein-coupled receptor 137B (GPR137B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204155L4 | Lenti ORF clone of Human G protein-coupled receptor 137B (GPR137B), mGFP tagged |
CNY 6,056.00 |
|
| RG204155 | GPR137B (tGFP-tagged) - Human G protein-coupled receptor 137B (GPR137B) |
CNY 5,256.00 |
|
| SC319632 | GPR137B (untagged)-Human G protein-coupled receptor 137B (GPR137B) |
CNY 3,656.00 |
