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Claudin 2 (CLDN2) (NM_020384) Human Tagged ORF Clone
CAT＃: RC204199
CLDN2 (Myc-DDK-tagged)-Human claudin 2 (CLDN2), transcript variant 1
ORF Plasmid: tGFP
"NM_020384" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OAZON |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTCTTGGCCTCCAACTTGTGGGCTACATCCTAGGCCTTCTGGGGCTTTTGGGCACACTGGTTG CCATGCTGCTCCCCAGCTGGAAAACAAGTTCTTATGTCGGTGCCAGCATTGTGACAGCAGTTGGCTTCTC CAAGGGCCTCTGGATGGAATGTGCCACACACAGCACAGGCATCACCCAGTGTGACATCTATAGCACCCTT CTGGGCCTGCCCGCTGACATCCAGGCTGCCCAGGCCATGATGGTGACATCCAGTGCAATCTCCTCCCTGG CCTGCATTATCTCTGTGGTGGGCATGAGATGCACAGTCTTCTGCCAGGAATCCCGAGCCAAAGACAGAGT GGCGGTAGCAGGTGGAGTCTTTTTCATCCTTGGAGGCCTCCTGGGATTCATTCCTGTTGCCTGGAATCTT CATGGGATCCTACGGGACTTCTACTCACCACTGGTGCCTGACAGCATGAAATTTGAGATTGGAGAGGCTC TTTACTTGGGCATTATTTCTTCCCTGTTCTCCCTGATAGCTGGAATCATCCTCTGCTTTTCCTGCTCATC CCAGAGAAATCGCTCCAACTACTACGATGCCTACCAAGCCCAACCTCTTGCCACAAGGAGCTCTCCAAGG CCTGGTCAACCTCCCAAAGTCAAGAGTGAGTTCAATTCCTACAGCCTGACAGGGTATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204199 protein sequence
Red=Cloning site Green=Tags(s) MASLGLQLVGYILGLLGLLGTLVAMLLPSWKTSSYVGASIVTAVGFSKGLWMECATHSTGITQCDIYSTL LGLPADIQAAQAMMVTSSAISSLACIISVVGMRCTVFCQESRAKDRVAVAGGVFFILGGLLGFIPVAWNL HGILRDFYSPLVPDSMKFEIGEALYLGIISSLFSLIAGIILCFSCSSQRNRSNYYDAYQAQPLATRSSPR PGQPPKVKSEFNSYSLTGYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020384 |
| ORF Size | 690 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020384.4 |
| RefSeq Size | 2998 bp |
| RefSeq ORF | 693 bp |
| Locus ID | 9075 |
| UniProt ID | P57739 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
| MW | 24.5 kDa |
| Gene Summary | This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Abstract 2627: Opposite functions of Claudin-2 involving Wnt signaling in liver cancer cells
,Koga, H;Imamura, Y;Nakamura, T;Iwamoto, H;Sakaue, T;Masuda, A;Tanaka, T;Nakano, D;Suzuki, H;Yano, H;Torimura, T;,
Tumor Biology
[Claudin 2]
|
|
Caveolin 1 is Associated with Upregulated Claudin 2 in Necrotizing Enterocolitis
,null,
Scientific Reports
,PubMed ID 30899070
[Claudin 2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204199L1 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204199L2 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204199L3 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204199L4 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG204199 | CLDN2 (tGFP-tagged) - Human claudin 2 (CLDN2), transcript variant 1 |
CNY 4,000.00 |
|
| SC304755 | CLDN2 (untagged)-Human claudin 2 (CLDN2), transcript variant 1 |
CNY 3,990.00 |
|
| SC320930 | CLDN2 (untagged)-Human claudin 2 (CLDN2), transcript variant 1 |
CNY 2,400.00 |
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