United States
Germany
Japan
United Kingdom
China
MED15 (NM_015889) Human Tagged ORF Clone
CAT＃: RC204224
MED15 (Myc-DDK-tagged)-Human mediator complex subunit 15 (MED15), transcript variant 2
ORF Plasmid: tGFP
"NM_015889" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 5,480.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTTTCCGGGCAAGAGACCGACTGGCGGAGCACCGCCTTCCGGCAGAAGCTGGTCAGTCAAATCG AGGATGCCATGAGGAAAGCTGGTGTGGCACACAGTAAATCCAGCAAGGATATGGAGAGCCATGTTTTCCT GAAGGCCAAGACCCGGGACGAATACCTTTCTCTCGTGGCCAGGCTCATTATCCATTTTCGAGACATTCAT AACAAGAAATCTCAAGCTTCCGTCAGTGATCCTATGAATGCACTCCAGAGCCTGACTGGCGGACCTGCTG CGGGAGCCGCTGGAATTGGCATGCCTCCTCGGGGCCCGGGACAGTCTCTGGGCGGGATGGGTAGCCTTGG TGCCATGGGACAGCCAATGTCTCTCTCAGGGCAGCCGCCTCCTGGGACCTCGGGGATGGCCCCTCACAGC ATGGCTGTCGTGTCTACGGCAACTCCACAGACCCAGCTGCAGCTCCAGCAGGTGGCGCTGCAGCAGCAGC AGCAACAGCAGCAGTTCCAGCAGCAGCAGCAGGCGGCGCTACAGCAGCAGCAGCAGCAGCAGCAACAGCA GCAGTTCCAGGCTCAGCAGAGTGCCATGCAGCAGCAGTTCCAAGCAGTAGTGCAGCAGCAGCAGCAGCTC CAGCAGCAGCAGCAGCAGCAGCAGCATCTAATTAAATTGCATCATCAAAATCAGCAACAGATACAGCAGC AGCAACAGCAGCTGCAGCGAATAGCACAGCTGCAGCTCCAACAACAGCAACAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGCAGGCTTTGCAGGCCCAGCCACCAATTCAGCAGCCACCGATGCAGCAGCCACAGCCT CCGCCCTCCCAGGCTCTGCCCCAGCAGCTGCAGCAGATGCATCACACACAGCACCACCAGCCGCCACCAC AGCCCCAGCAGCCTCCAGTTGCTCAGAACCAACCATCACAACTCCCGCCACAGTCGCAGACCCAGCCTTT GGTGTCACAGGCGCAAGCTCTCCCTGGACAAATGTTGTATACCCAACCACCACTGAAATTTGTCCGAGCT CCGATGGTGGTGCAGCAGCCCCCAGTGCAGCCCCAGGTGCAGCAGCAGCAGACAGCAGTACAGACAGCTC AGGCTGCCCAGATGGTGGCTCCCGGAGTCCAGGTCAGCCAGAGCAGCCTCCCCATGCTGTCCTCGCCGTC ACCGGGCCAGCAGGTGCAGACCCCGCAGTCGATGCCCCCTCCCCCCCAGCCGTCCCCGCAGCCCGGCCAG CCCAGCTCACAGCCCAACTCCAACGTCAGCTCTGGCCCTGCCCCATCTCCCAGTAGCTTCCTGCCCAGCC CCTCACCGCAGCCCTCCCAGAGCCCAGTGACGGCGCGGACCCCACAGAACTTCAGTGTCCCCTCACCTGG ACCTTTAAACACACCTGTGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCCAGCCAGGCTGAGGAGCAG CAGTACCTGGACAAGCTGAAGCAGCTGTCGAAGTACATCGAGCCCCTGCGCCGCATGATCAACAAGATCG ACAAGAACGAAGACAGAAAAAAGGACCTGAGTAAGATGAAGAGCCTTCTGGACATTCTGACAGACCCCTC GAAGCGGTGTCCCCTGAAGACCTTGCAAAAGTGTGAGATCGCCCTGGAGAAACTCAAGAATGACATGGCG GTGCCCACTCCCCCACCGCCCCCGGTGCCACCGACCAAACAGCAGTACCTATGCCAGCCGCTCCTGGATG CCGTCCTGGCCAACATCCGCTCACCTGTCTTCAACCATTCCCTGTACCGCACATTCGTTCCAGCCATGAC CGCCATTCACGGCCCACCCATCACGGCCCCAGTGGTGTGCACCCGGAAGCGCAGGCTTGAGGATGATGAG CGGCAGAGCATCCCCAGTGTGCTCCAGGGTGAGGTGGCCAGGCTGGACCCCAAGTTCCTGGTAAACCTGG ACCCTTCTCACTGCAGCAACAATGGCACTGTCCACCTGATCTGCAAGCTGGATGACAAGGACCTCCCAAG TGTGCCACCACTGGAGCTCAGTGTGCCCGCTGACTATCCTGCCCAAAGCCCGCTGTGGATAGACCGGCAG TGGCAGTACGACGCCAACCCCTTCCTCCAGTCGGTGCACCGCTGCATGACCTCCAGGCTGCTGCAGCTCC CGGACAAGCACTCGGTCACCGCCTTGCTCAACACCTGGGCCCAGAGCGTCCACCAGGCCTGCCTCTCAGC CGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204224 protein sequence
Red=Cloning site Green=Tags(s) MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH NKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHS MAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQL QQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQP PPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRA PMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQ PSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQ QYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMA VPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDE RQSIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQ WQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015889 |
| ORF Size | 2244 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015889.5 |
| RefSeq Size | 3331 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 51586 |
| UniProt ID | Q96RN5 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 82.6 kDa |
| Gene Summary | The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204224L3 | Lenti ORF clone of Human mediator complex subunit 15 (MED15), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204224L4 | Lenti ORF clone of Human mediator complex subunit 15 (MED15), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204224 | MED15 (tGFP-tagged) - Human mediator complex subunit 15 (MED15), transcript variant 2 |
CNY 7,080.00 |
|
| SC114575 | MED15 (untagged)-Human mediator complex subunit 15 (MED15), transcript variant 2 |
CNY 5,488.00 |
|
| SC320784 | MED15 (untagged)-Human mediator complex subunit 15 (MED15), transcript variant 2 |
CNY 5,488.00 |
