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SGK3 (NM_013257) Human Tagged ORF Clone
CAT＃: RC204416
SGK3 (Myc-DDK-tagged)-Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1
ORF Plasmid: tGFP
"NM_013257" in other vectors (8)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CISK; SGK2; SGKL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204416 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAGAGATCACACCATGGACTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAAC ACAGAGAGAAAAAGAAGAGGTTTACTGTTTATAAAGTTCTGGTTTCAGTGGGAAGAAGTGAATGGTTTGT CTTCAGGAGATATGCAGAGTTTGATAAACTTTATAACACTTTAAAAAAACAGTTTCCTGCTATGGCCCTG AAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGACGAGCAGGAC TAAACGAATTCATTCAGAACCTAGTTAGGTATCCAGAACTTTATAACCATCCAGATGTCAGAGCATTCCT TCAAATGGACAGTCCAAAACACCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTTCTCAGAAGCTA CACTCTACCTCACAGAACATCAACCTGGGACCGTCTGGAAATCCTCATGCCAAACCAACTGACTTTGATT TCTTAAAAGTTATTGGAAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATT TTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAA CGTAATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAACTG AAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTTTCCACTTACAAAGAGAACGGTC CTTTCCTGAGCACAGAGCTAGGTTTTACGCTGCTGAAATTGCTAGTGCATTGGGTTACTTACATTCCATC AAAATAGTATACAGAGACTTGAAACCAGAAAATATTCTTTTGGATTCAGTAGGACATGTTGTCTTAACAG ATTTTGGGCTTTGTAAAGAAGGAATTGCTATTTCTGACACCACTACCACATTTTGTGGGACACCAGAGTA TCTTGCACCTGAAGTAATTAGAAAACAGCCCTATGACAATACTGTAGATTGGTGGTGCCTTGGGGCTGTT CTGTATGAAATGCTGTATGGATTGCCTCCTTTTTATTGCCGAGATGTTGCTGAAATGTATGACAATATCC TTCACAAACCCCTAAGTTTGAGGCCAGGAGTGAGTCTTACAGCCTGGTCCATTCTGGAAGAACTCCTAGA AAAAGACAGGCAAAATCGACTTGGTGCCAAGGAAGACTTTCTTGAAATTCAGAATCATCCTTTTTTTGAA TCACTCAGCTGGGCTGACCTTGTACAAAAGAAGATTCCACCACCATTTAATCCTAATGTGGCTGGACCAG ATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGA CTATTCTATAGTGAATGCCAGTGTATTGGAGGCAGATGATGCATTCGTTGGTTTCTCTTATGCACCTCCT TCAGAAGACTTATTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204416 protein sequence
Red=Cloning site Green=Tags(s) MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRRYAEFDKLYNTLKKQFPAMAL KIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKL HSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAE RNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSI KIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAV LYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFE SLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPP SEDLFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013257 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013257.4 |
| RefSeq Size | 4206 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 23678 |
| UniProt ID | Q96BR1 |
| Domains | pkinase, S_TK_X, TyrKc, PX, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 57.1 kDa |
| Gene Summary | This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204416L1 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204416L2 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204416L3 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204416L4 | Lenti ORF clone of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204416 | SGK3 (tGFP-tagged) - Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 |
CNY 5,256.00 |
|
| SC115312 | SGK3 (untagged)-Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 |
CNY 3,656.00 |
|
| SC322464 | SGK3 (untagged)-Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 |
CNY 3,656.00 |
|
| SC323383 | SGK3 (untagged)-Kinase deficient mutant (K191M) of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 |
CNY 3,656.00 |
