MIR16 (GDE1) (NM_016641) Human Tagged ORF Clone
CAT#: RC204923
GDE1 (Myc-DDK-tagged)-Human glycerophosphodiester phosphodiesterase 1 (GDE1)
ORF Plasmid: tGFP
"NM_016641" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 363E6.2; MIR16 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204923 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGTGGGAGGACCAGGGCGGCCTCCTGGGCCCTTTCTCCTTCCTGCTGCTAGTGCTGCTGCTGG TGACGCGGAGCCCGGTCAATGCCTGCCTCCTCACCGGCAGCCTCTTCGTTCTACTGCGCGTCTTCAGCTT TGAGCCGGTGCCCTCTTGCAGGGCCCTGCAGGTGCTCAAGCCCCGGGACCGCATTTCTGCCATCGCCCAC CGTGGCGGCAGCCACGACGCGCCCGAGAACACGCTGGCGGCCATTCGGCAGGCAGCTAAGAATGGAGCAA CAGGCGTGGAGTTGGACATTGAGTTTACTTCTGACGGGATTCCTGTCTTAATGCACGATAACACAGTAGA TAGGACGACTGATGGGACTGGGCGATTGTGTGATTTGACATTTGAACAAATTAGGAAGCTGAATCCTGCA GCAAACCACAGACTCAGGAATGATTTCCCTGATGAAAAGATCCCTACCCTAAGGGAAGCTGTTGCAGAGT GCCTAAACCATAACCTCACAATCTTCTTTGATGTCAAAGGCCATGCACACAAGGCTACTGAGGCTCTAAA GAAAATGTATATGGAATTTCCTCAACTGTATAATAATAGTGTGGTCTGTTCTTTCTTGCCAGAAGTTATC TACAAGATGAGACAAACAGATCGGGATGTAATAACAGCATTAACTCACAGACCTTGGAGCCTAAGCCATA CAGGAGATGGGAAACCACGCTATGATACTTTCTGGAAACATTTTATATTTGTTATGATGGACATTTTGCT CGATTGGAGCATGCATAATATCTTGTGGTACCTGTGTGGAATTTCAGCTTTCCTCATGCAAAAGGATTTT GTATCCCCGGCCTACTTGAAGAAGTGGTCAGCTAAAGGAATCCAGGTTGTTGGTTGGACTGTTAATACCT TTGATGAAAAGAGTTACTACGAATCCCATCTTGGTTCCAGCTATATCACTGACAGCATGGTAGAAGACTG CGAACCTCACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204923 protein sequence
Red=Cloning site Green=Tags(s) MWLWEDQGGLLGPFSFLLLVLLLVTRSPVNACLLTGSLFVLLRVFSFEPVPSCRALQVLKPRDRISAIAH RGGSHDAPENTLAAIRQAAKNGATGVELDIEFTSDGIPVLMHDNTVDRTTDGTGRLCDLTFEQIRKLNPA ANHRLRNDFPDEKIPTLREAVAECLNHNLTIFFDVKGHAHKATEALKKMYMEFPQLYNNSVVCSFLPEVI YKMRQTDRDVITALTHRPWSLSHTGDGKPRYDTFWKHFIFVMMDILLDWSMHNILWYLCGISAFLMQKDF VSPAYLKKWSAKGIQVVGWTVNTFDEKSYYESHLGSSYITDSMVEDCEPHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016641 |
ORF Size | 993 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016641.4 |
RefSeq Size | 2960 bp |
RefSeq ORF | 996 bp |
Locus ID | 51573 |
UniProt ID | Q9NZC3 |
Domains | GDPD |
Protein Families | Transmembrane |
Protein Pathways | Glycerophospholipid metabolism |
MW | 37.7 kDa |
Gene Summary | Has glycerophosphoinositol phosphodiesterase activity. Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204923L3 | Lenti ORF clone of Human glycerophosphodiester phosphodiesterase 1 (GDE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204923L4 | Lenti ORF clone of Human glycerophosphodiester phosphodiesterase 1 (GDE1), mGFP tagged |
CNY 5,890.00 |
|
RG204923 | GDE1 (tGFP-tagged) - Human glycerophosphodiester phosphodiesterase 1 (GDE1) |
CNY 4,000.00 |
|
SC108185 | GDE1 (untagged)-Human glycerophosphodiester phosphodiesterase 1 (GDE1) |
CNY 2,400.00 |