Parvin alpha (PARVA) (NM_018222) Human Tagged ORF Clone

CAT#: RC205086

PARVA (Myc-DDK-tagged)-Human parvin, alpha (PARVA)

ORF Plasmid: DDK tGFP



  "NM_018222" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PARVA mouse monoclonal antibody,clone OTI1H2
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CH-ILKBP; MXRA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205086 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCTCCCCGCAGAAGTCGCCTTCTGTCCCCAAGTCTCCCACTCCCAAGTCGCCCCCGTCCCGCA
AGAAAGATGATTCCTTCTTGGGGAAACTCGGAGGGACCCTGGCCCGGAGGAAGAAAGCCAAGGAGGTGTC
CGAGCTGCAGGAGGAGGGAATGAACGCCATCAACCTGCCCCTCAGCCCAATTCCCTTTGAGCTGGACCCC
GAGGACACGATGCTGGAGGAGAATGAGGTGCGAACAATGGTGGATCCAAACTCACGCAGTGACCCCAAGC
TTCAAGAACTGATGAAGGTATTAATTGACTGGATTAATGATGTGTTGGTTGGAGAAAGAATCATTGTGAA
AGACCTAGCTGAAGATTTGTATGATGGACAAGTCCTGCAGAAGCTTTTCGAGAAACTGGAGAGTGAGAAG
CTAAATGTGGCTGAGGTCACCCAGTCAGAGATTGCTCAGAAGCAAAAACTGCAGACTGTCCTGGAGAAGA
TCAATGAAACCCTGAAACTTCCTCCCAGGAGCATCAAGTGGAATGTGGATTCTGTTCATGCCAAGAGCCT
GGTGGCCATCTTACACCTGCTCGTTGCTCTGTCTCAGTATTTCCGCGCACCAATTCGACTCCCAGACCAT
GTTTCCATCCAAGTGGTTGTGGTCCAGAAACGAGAAGGAATCCTCCAGTCTCGGCAAATCCAAGAGGAAA
TAACTGGTAACACAGAGGCTCTTTCCGGGAGGCATGAACGTGATGCCTTTGACACCTTGTTCGACCATGC
CCCAGACAAGCTGAATGTGGTGAAAAAGACACTCATCACTTTCGTGAACAAGCACCTGAATAAACTGAAC
CTGGAGGTCACAGAACTGGAAACCCAGTTTGCAGATGGGGTGTACCTGGTGCTGCTCATGGGGCTCCTGG
AGGGCTACTTTGTGCCCCTGCACAGCTTCTTCCTGACCCCGGACAGCTTTGAACAGAAGGTCTTGAATGT
CTCCTTTGCCTTTGAGCTCATGCAAGATGGAGGGTTGGAAAAGCCAAAACCGCGGCCAGAAGACATAGTC
AACTGTGACCTGAAATCTACACTACGAGTGTTGTACAACCTCTTCACCAAGTACCGTAACGTGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205086 protein sequence
Red=Cloning site Green=Tags(s)

MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSELQEEGMNAINLPLSPIPFELDP
EDTMLEENEVRTMVDPNSRSDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEK
LNVAEVTQSEIAQKQKLQTVLEKINETLKLPPRSIKWNVDSVHAKSLVAILHLLVALSQYFRAPIRLPDH
VSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLN
LEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIV
NCDLKSTLRVLYNLFTKYRNVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018222
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018222.5
RefSeq Size 8719 bp
RefSeq ORF 1119 bp
Locus ID 55742
UniProt ID Q9NVD7
Domains CH
Protein Pathways Focal adhesion
MW 42.2 kDa
Gene Summary This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq, Dec 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...