DPP8 (NM_197961) Human Tagged ORF Clone

CAT#: RC205236

DPP8 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4

ORF Plasmid: DDK tGFP



  "NM_197961" in other vectors (6)

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CNY 6,208.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DPP8 mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DP8; DPRP-1; DPRP1; MST097; MSTP097; MSTP135; MSTP141
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205236 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAAGAGATCTGAGCAGATGAAAATAAAATCAGGAAAATGCAACATGGCAGCAGCAATGGAAACAG
AACAGCTGGGTGTTGAGATATTTGAAACTGCGGACTGTGAGGAGAATATTGAATCACAGGATCGGCCTAA
ATTGGAGCCTTTTTATGTTGAGCGGTATTCCTGGAGTCAGCTTAAAAAGCTGCTTGCCGATACCAGAAAA
TATCATGGCTACATGATGGCTAAGGCACCACATGATTTCATGTTTGTGAAGAGGAATGATCCAGATGGAC
CTCATTCAGACAGAATCTATTACCTTGCCATGTCTGGTGAGAACAGAGAAAATACACTGTTTTATTCTGA
AATTCCCAAAACTATCAATAGAGCAGCAGTCTTAATGCTCTCTTGGAAGCCTCTTTTGGATCTTTTTCAG
GCAACACTGGACTATGGAATGTATTCTCGAGAAGAAGAACTATTAAGAGAAAGAAAACGCATTGGAACAG
TCGGAATTGCTTCTTACGATTATCACCAAGGAAGTGGAACATTTCTGTTTCAAGCCGGTAGTGGAATTTA
TCACGTAAAAGATGGAGGGCCACAAGGATTTACGCAACAACCTTTAAGGCCCAATCTAGTGGAAACTAGT
TGTCCCAACATACGGATGGATCCAAAATTATGCCCTGCTGATCCAGACTGGATTGCTTTTATACATAGCA
ACGATATTTGGATATCTAACATCGTAACCAGAGAAGAAAGGAGACTCACTTATGTGCACAATGAGCTAGC
CAACATGGAAGAAGATGCCAGATCAGCTGGAGTCGCTACCTTTGTTCTCCAAGAAGAATTTGATAGATAT
TCTGGCTATTGGTGGTGTCCAAAAGCTGAAACAACTCCCAGTGGTGGTAAAATTCTTAGAATTCTATATG
AAGAAAATGATGAATCTGAGGTGGAAATTATTCATGTTACATCCCCTATGTTGGAAACAAGGAGGGCAGA
TTCATTCCGTTATCCTAAAACAGGTACAGCAAATCCTAAAGTCACTTTTAAGATGTCAGAAATAATGATT
GATGCTGAAGGAAGGATCATAGATGTCATAGATAAGGAACTAATTCAACCTTTTGAGATTCTATTTGAAG
GAGTTGAATATATTGCCAGAGCTGGATGGACTCCTGAGGGAAAATATGCTTGGTCCATCCTACTAGATCG
CTCCCAGACTCGCCTACAGATAGTGTTGATCTCACCTGAATTATTTATCCCAGTAGAAGATGATGTTATG
GAAAGGCAGAGACTCATTGAGTCAGTGCCTGATTCTGTGACGCCACTAATTATCTATGAAGAAACAACAG
ACATCTGGATAAATATCCATGACATCTTTCATGTTTTTCCCCAAAGTCACGAAGAGGAAATTGAGTTTAT
TTTTGCCTCTGAATGCAAAACAGGTTTCCGTCATTTATACAAAATTACATCTATTTTAAAGGAAAGCAAA
TATAAACGATCCAGTGGTGGGCTGCCTGCTCCAAGTGATTTCAAGTGTCCTATCAAAGAGGAGATAGCAA
TTACCAGTGGTGAATGGGAAGTTCTTGGCCGGCATGGATCTAATATCCAAGTTGATGAAGTCAGAAGGCT
GGTATATTTTGAAGGCACCAAAGACTCCCCTTTAGAGCATCACCTGTACGTAGTCAGTTACGTAAATCCT
GGAGAGGTGACAAGGCTGACTGACCGTGGCTACTCACATTCTTGCTGCATCAGTCAGCACTGTGACTTCT
TTATAAGTAAGTATAGTAACCAGAAGAATCCACACTGTGTGTCCCTTTACAAGCTATCAAGTCCTGAAGA
TGACCCAACTTGCAAAACAAAGGAATTTTGGGCCACCATTTTGGATTCAGCAGGTCCTCTTCCTGACTAT
ACTCCTCCAGAAATTTTCTCTTTTGAAAGTACTACTGGATTTACATTGTATGGGATGCTCTACAAGCCTC
ATGATCTACAGCCTGGAAAGAAATATCCTACTGTGCTGTTCATATATGGTGGTCCTCAGGTGCAGTTGGT
GAATAATCGGTTTAAAGGAGTCAAGTATTTCCGCTTGAATACCCTAGCCTCTCTAGGTTATGTGGTTGTA
GTGATAGACAACAGGGGATCCTGTCACCGAGGGCTTAAATTTGAAGGCGCCTTTAAATATAAAATGGTTG
CTATTGCTGGGGCCCCAGTCACTCTGTGGATCTTCTATGATACAGGATACACGGAACGTTATATGGGTCA
CCCTGACCAGAATGAACAGGGCTATTACTTAGGATCTGTGGCCATGCAAGCAGAAAAGTTCCCCTCTGAA
CCAAATCGTTTACTGCTCTTACATGGTTTCCTGGATGAGAATGTCCATTTTGCACATACCAGTATATTAC
TGAGTTTTTTAGTGAGGGCTGGAAAGCCATATGATTTACAGATCTATCCTCAGGAGAGACACAGCATAAG
AGTTCCTGAATCGGGAGAACATTATGAACTGCATCTTTTGCACTACCTTCAAGAAAACCTTGGATCACGT
ATTGCTGCTCTAAAAGTGATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205236 protein sequence
Red=Cloning site Green=Tags(s)

MWKRSEQMKIKSGKCNMAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQLKKLLADTRK
YHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEIPKTINRAAVLMLSWKPLLDLFQ
ATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSGTFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETS
CPNIRMDPKLCPADPDWIAFIHSNDIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRY
SGYWWCPKAETTPSGGKILRILYEENDESEVEIIHVTSPMLETRRADSFRYPKTGTANPKVTFKMSEIMI
DAEGRIIDVIDKELIQPFEILFEGVEYIARAGWTPEGKYAWSILLDRSQTRLQIVLISPELFIPVEDDVM
ERQRLIESVPDSVTPLIIYEETTDIWINIHDIFHVFPQSHEEEIEFIFASECKTGFRHLYKITSILKESK
YKRSSGGLPAPSDFKCPIKEEIAITSGEWEVLGRHGSNIQVDEVRRLVYFEGTKDSPLEHHLYVVSYVNP
GEVTRLTDRGYSHSCCISQHCDFFISKYSNQKNPHCVSLYKLSSPEDDPTCKTKEFWATILDSAGPLPDY
TPPEIFSFESTTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYVVV
VIDNRGSCHRGLKFEGAFKYKMVAIAGAPVTLWIFYDTGYTERYMGHPDQNEQGYYLGSVAMQAEKFPSE
PNRLLLLHGFLDENVHFAHTSILLSFLVRAGKPYDLQIYPQERHSIRVPESGEHYELHLLHYLQENLGSR
IAALKVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_197961
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_197961.4
RefSeq Size 4079 bp
RefSeq ORF 2544 bp
Locus ID 54878
UniProt ID Q6V1X1
Protein Families Druggable Genome, Protease, Transmembrane
MW 97.5 kDa
Gene Summary This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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