ICAM5 (NM_003259) Human Tagged ORF Clone

CAT#: RC205369

ICAM5 (Myc-DDK-tagged)-Human intercellular adhesion molecule 5, telencephalin (ICAM5)

ORF Plasmid: DDK tGFP



  "NM_003259" in other vectors (4)

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CNY 6,768.00

CNY 7,130.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-ICAM5 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms TLCN; TLN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205369 representing NM_003259
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGGCCTTCGCCAGGGCTGCGCCGGGCGCTACTCGGCCTCTGGGCTGCTCTGGGCCTGGGGCTCT
TCGGCCTCTCAGCGGTCTCGCAGGAGCCCTTCTGGGCGGACCTGCAGCCTCGCGTGGCGTTCGTGGAGCG
CGGGGGCTCGCTGTGGCTGAATTGCAGCACCAACTGCCCTCGGCCGGAGCGCGGTGGCCTGGAGACCTCG
CTGCGCCGAAACGGGACCCAGAGGGGTTTGCGTTGGTTGGCGCGGCAGCTGGTGGACATTCGCGAGCCGG
AGACTCAGCCCGTCTGCTTCTTCCGCTGCGCGCGGCGCACACTACAGGCGCGTGGGCTCATTCGCACTTT
CCAGCGACCAGATCGCGTAGAGCTGATGCCGCTGCCTCCCTGGCAGCCGGTGGGCGAGAACTTCACCCTG
AGCTGTAGGGTCCCCGGCGCCGGGCCCCGTGCGAGCCTCACGCTGACCCTGCTGCGGGGCGCCCAGGAGC
TGATCCGCCGCAGCTTCGCCGGTGAACCACCCCGAGCGCGGGGCGCGGTGCTCACAGCCACGGTACTGGC
TCGGAGGGAGGACCATGGAGCCAATTTCTCGTGTCGCGCCGAGCTGGACCTGCGGCCGCACGGACTGGGA
CTGTTTGAAAACAGCTCGGCCCCCAGAGAGCTCCGAACCTTCTCCCTGTCTCCGGATGCCCCGCGCCTCG
CTGCTCCCCGGCTCTTGGAAGTTGGCTCGGAAAGGCCCGTGAGCTGCACTCTGGACGGACTGTTTCCAGC
CTCAGAGGCCAGGGTCTACCTCGCACTGGGGGACCAGAATCTGAGTCCTGATGTCACCCTCGAAGGGGAC
GCATTCGTGGCCACTGCCACAGCCACAGCTAGCGCAGAGCAGGAGGGTGCCAGGCAGCTGGTCTGCAACG
TCACCCTGGGGGGCGAAAACCGGGAGACCCGGGAGAACGTGACCATCTACAGCTTCCCGGCACCACTCCT
GACCCTGAGCGAACCCAGCGTCTCCGAGGGGCAGATGGTGACAGTAACCTGCGCAGCTGGGGCCCAAGCT
CTGGTCACACTGGAGGGAGTTCCAGCCGCGGTCCCGGGGCAGCCCGCCCAGCTTCAGCTAAATGCCACCG
AGAACGACGACAGACGCAGCTTCTTCTGCGACGCCACCCTCGATGTGGACGGGGAGACCCTGATCAAGAA
CAGGAGCGCAGAGCTTCGTGTCCTATACGCTCCCCGGCTAGACGATTCGGACTGCCCCAGGAGTTGGACG
TGGCCCGAGGGCCCAGAGCAGACGCTGCGCTGCGAGGCCCGCGGGAACCCAGAACCCTCAGTGCACTGTG
CGCGCTCCGACGGCGGGGCCGTGCTGGCTCTGGGCCTGCTGGGTCCAGTCACTCGGGCGCTCTCAGGCAC
TTACCGCTGCAAGGCGGCCAATGATCAAGGCGAGGCGGTCAAGGACGTAACGCTAACGGTGGAGTACGCA
CCAGCGCTGGACAGCGTGGGCTGCCCAGAACGCATTACTTGGCTGGAGGGAACAGAAGCCTCGCTGAGCT
GTGTGGCGCACGGGGTACCGCCGCCTGATGTGATCTGCGTGCGCTCTGGAGAACTCGGGGCCGTCATCGA
GGGGCTGTTGCGTGTGGCCCGGGAGCATGCGGGCACTTACCGCTGCGAAGCCACCAACCCTCGGGGCTCT
GCGGCCAAAAATGTGGCCGTCACGGTGGAATATGGCCCCAGGTTTGAGGAGCCGAGCTGCCCCAGCAATT
GGACATGGGTGGAAGGATCTGGGCGCCTGTTTTCCTGTGAGGTCGATGGGAAGCCACAGCCAAGCGTGAA
GTGCGTGGGCTCCGGGGGCGCCACTGAGGGGGTGCTGCTGCCGCTGGCACCCCCAGACCCTAGTCCCAGA
GCTCCCAGAATCCCTAGAGTCCTGGCACCCGGTATCTACGTCTGCAACGCCACCAACCGCCACGGCTCCG
TGGCCAAAACAGTCGTCGTGAGCGCGGAGTCGCCACCGGAGATGGATGAATCTACCTGCCCAAGTCACCA
GACGTGGCTGGAAGGGGCTGAGGCTTCCGCGCTGGCCTGCGCCGCCCGGGGTCGCCCTTCCCCAGGAGTG
CGCTGCTCTCGGGAAGGCATCCCATGGCCTGAGCAGCAGCGCGTGTCCCGAGAGGACGCGGGCACTTACC
ACTGTGTGGCCACCAATGCGCATGGCACGGACTCCCGGACCGTCACTGTGGGCGTGGAATACCGGCCAGT
GGTGGCCGAACTTGCTGCCTCGCCCCCTGGAGGCGTGCGCCCAGGAGGAAACTTCACGTTGACCTGCCGC
GCGGAGGCCTGGCCTCCAGCCCAGATCAGCTGGCGCGCGCCCCCGGGGGCCCTCAACATCGGCCTGTCGA
GCAACAACAGCACACTGAGCGTGGCAGGCGCCATGGGAAGCCACGGCGGCGAGTACGAGTGCGCAGCCAC
CAACGCGCACGGGCGCCACGCGCGGCGCATCACGGTGCGCGTGGCCGGTCCGTGGCTATGGGTCGCCGTG
GGCGGCGCGGCGGGGGGCGCGGCGCTGCTGGCCGCGGGGGCCGGCCTGGCCTTCTACGTGCAGTCCACCG
CCTGCAAGAAGGGCGAGTACAACGTGCAGGAGGCCGAGAGCTCAGGCGAGGCCGTGTGTCTGAACGGAGC
GGGCGGCGGCGCTGGCGGGGCGGCAGGCGCGGAGGGCGGACCCGAGGCGGCGGGGGGCGCGGCCGAGTCG
CCGGCGGAGGGCGAGGTCTTCGCCATACAGCTGACATCGGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205369 representing NM_003259
Red=Cloning site Green=Tags(s)

MPGPSPGLRRALLGLWAALGLGLFGLSAVSQEPFWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETS
LRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTL
SCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLG
LFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGD
AFVATATATASAEQEGARQLVCNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGAQA
LVTLEGVPAAVPGQPAQLQLNATENDDRRSFFCDATLDVDGETLIKNRSAELRVLYAPRLDDSDCPRSWT
WPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLGPVTRALSGTYRCKAANDQGEAVKDVTLTVEYA
PALDSVGCPERITWLEGTEASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPRGS
AAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKCVGSGGATEGVLLPLAPPDPSPR
APRIPRVLAPGIYVCNATNRHGSVAKTVVVSAESPPEMDESTCPSHQTWLEGAEASALACAARGRPSPGV
RCSREGIPWPEQQRVSREDAGTYHCVATNAHGTDSRTVTVGVEYRPVVAELAASPPGGVRPGGNFTLTCR
AEAWPPAQISWRAPPGALNIGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGPWLWVAV
GGAAGGAALLAAGAGLAFYVQSTACKKGEYNVQEAESSGEAVCLNGAGGGAGGAAGAEGGPEAAGGAAES
PAEGEVFAIQLTSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003259
ORF Size 2772 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003259.4
RefSeq Size 3005 bp
RefSeq ORF 2775 bp
Locus ID 7087
UniProt ID Q9UMF0
Protein Families Transmembrane
MW 97.1 kDa
Gene Summary The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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