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FEM 1C (FEM1C) (NM_020177) Human Tagged ORF Clone
CAT＃: RC205563
FEM1C (Myc-DDK-tagged)-Human fem-1 homolog c (C. elegans) (FEM1C)
ORF Plasmid: tGFP
"NM_020177" in other vectors (4)
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CNY 4,592.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EUROIMAGE686608; EUROIMAGE783647; FEM1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205563 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTAAAGACAGCAGTATTTAACGCAGCTCGGGATGGCAAACTCCGGCTTCTCACCAAATTGTTGG CAAGCAAATCCAAAGAGGAGGTTTCCTCCTTGATCTCTGAAAAAACAAATGGGGCCACGCCACTCTTGAT GGCCGCCAGGTATGGGCACCTTGACATGGTGGAATTCCTCCTAGAGCAATGCAGTGCCTCCATAGAAGTT GGGGGCTCCGTCAATTTTGATGGCGAAACCATTGAGGGGGCTCCCCCTTTATGGGCCGCTTCTGCAGCAG GACATCTGAAGGTGGTCCAGTCCTTGTTAAATCATGGAGCATCTGTCAACAACACGACTTTAACCAATTC AACTCCTCTTCGAGCTGCGTGTTTCGATGGCCATTTGGAAATAGTGAAGTACCTTGTAGAACACAAAGCT GATTTGGAAGTGTCAAACCGACATGGGCATACGTGCTTGATGATTTCATGTTACAAAGGACATAAAGAGA TTGCTCAGTATTTACTTGAAAAGGGGGCAGATGTTAATAGAAAAAGTGTCAAAGGTAATACTGCATTGCA TGATTGTGCAGAATCTGGAAGTTTGGACATCATGAAGATGCTTCTTATGTATTGTGCCAAGATGGAAAAG GATGGTTATGGAATGACTCCCCTTCTCTCAGCAAGTGTGACTGGTCACACAAATATTGTGGATTTTCTGA CACACCATGCACAGACCAGCAAGACAGAACGTATTAATGCTCTAGAGCTTCTGGGAGCTACATTTGTAGA CAAAAAAAGAGATCTGCTTGGGGCTTTGAAATACTGGAAAAAGGCAATGAACATGAGGTACAGTGATAGG ACTAATATTATTAGTAAACCAGTGCCACAGACACTAATAATGGCTTATGATTATGCCAAGGAAGTGAACA GTGCAGAAGAGCTAGAAGGTCTTATTGCTGATCCTGATGAGATGAGAATGCAGGCACTATTAATCAGAGA ACGTATTCTTGGTCCTTCTCATCCTGATACCTCTTACTATATTAGATATAGAGGCGCTGTCTATGCAGAC TCTGGAAATTTCAAACGATGCATCAACCTATGGAAGTATGCTTTGGATATGCAGCAGAGCAATTTGGATC CTTTAAGCCCAATGACAGCCAGCAGCTTATTATCTTTTGCAGAACTATTCTCCTTTATGCTACAGGATAG GGCTAAAGGCCTGCTGGGTACTACTGTTACATTTGATGATCTTATGGGCATACTTTGCAAAAGCGTCCTT GAAATAGAGCGAGCTATCAAACAAACTCAGTGTCCAGCTGACCCATTACAGTTAAATAAGGCCCTTTCTA TTATTTTGCACTTAATTTGCTTGTTAGAGAAAGTTCCTTGTACTCTAGAACAAGACCATTTCAAAAAGCA GACTATATACAGGTTTCTTAAGCTGCATCCAAGGGGAAAGAATAACTTCAGCCCTCTTCATCTGGCTGTG GACAAGAATACTACATGTGTAGGGCGGTACCCTGTTTGTAAATTTCCATCTCTACAAGTTACTGCAATAC TGATAGAATGTGGTGCTGATGTGAACGTCAGAGACTCGGATGACAACAGTCCCCTGCATATCGCTGCTCT TAACAACCATCCAGACATCATGAATCTCCTTATTAAATCAGGTGCACATTTTGATGCCACAAACTTGCAC AAACAAACTGCTAGTGACTTGCTGGATGAGAAGGAAATAGCTAAAAATTTAATCCAGCCTATAAATCATA CCACATTGCAGTGTCTTGCTGCTCGTGTCATAGTGAATCATAGAATATATTATAAAGGGCATATCCCAGA AAAGCTAGAGACTTTTGTTTCCCTTCATAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205563 protein sequence
Red=Cloning site Green=Tags(s) MDLKTAVFNAARDGKLRLLTKLLASKSKEEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEV GGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKA DLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEK DGYGMTPLLSASVTGHTNIVDFLTHHAQTSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDR TNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYAD SGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTTVTFDDLMGILCKSVL EIERAIKQTQCPADPLQLNKALSIILHLICLLEKVPCTLEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAV DKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNNHPDIMNLLIKSGAHFDATNLH KQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020177 |
| ORF Size | 1851 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020177.3 |
| RefSeq Size | 5813 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 56929 |
| UniProt ID | Q96JP0 |
| Domains | ANK |
| MW | 68.7 kDa |
| Gene Summary | Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205563L3 | Lenti ORF clone of Human fem-1 homolog c (C. elegans) (FEM1C), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205563L4 | Lenti ORF clone of Human fem-1 homolog c (C. elegans) (FEM1C), mGFP tagged |
CNY 6,460.00 |
|
| RG205563 | FEM1C (tGFP-tagged) - Human fem-1 homolog c (C. elegans) (FEM1C) |
CNY 6,192.00 |
|
| SC102558 | FEM1C (untagged)-Human fem-1 homolog c (C. elegans) (FEM1C) |
CNY 7,220.00 |
