BRUNOL6 (CELF6) (NM_052840) Human Tagged ORF Clone

CAT#: RC205606

CELF6 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 6 (CELF6), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_052840" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CELF6 mouse monoclonal antibody,clone OTI5G3
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BRUNOL6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205606 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGCGGCGCCGGGAGGGTCAGCGCAGCCCGCTGGCCCCGGCCCGCGCCTGGGTTTCAGCACCGCGG
ACAGCGGCGTCGGCATGAGCGGGCTAAACCCCGGTCCCGCCGTACCCATGAAGGACCACGACGCCATCAA
GCTCTTCGTGGGGCAGATCCCGCGGGGCTTGGACGAGCAGGACCTCAAGCCGCTGTTCGAGGAGTTCGGC
CGCATCTACGAGCTGACGGTGCTGAAGGACCGGCTCACCGGCCTCCACAAAGGCTGTGCCTTCCTCACCT
ACTGCGCCCGGGACTCTGCTCTCAAGGCCCAGAGTGCACTGCACGAGCAGAAGACCCTGCCAGGGATGAA
TCGTCCGATCCAAGTGAAGCCAGCTGCCAGTGAGGGCCGAGGAGAGGACCGAAAGCTGTTTGTGGGGATG
CTGGGCAAGCAGCAGGGTGAGGAGGACGTCAGACGCCTGTTCCAGCCCTTTGGCCACATCGAGGAGTGCA
CGGTCCTGCGGAGTCCTGACGGCACCAGTAAAGGCTGTGCCTTTGTGAAGTTCGGGAGTCAAGGGGAAGC
TCAGGCGGCCATCCGGGGTCTGCACGGCAGCCGGACCATGGCGGGCGCCTCGTCCAGCCTCGTGGTCAAG
CTGGCGGACACCGACCGGGAGCGCGCGCTGCGGCGGATGCAGCAGATGGCCGGCCACCTGGGCGCCTTCC
ACCCCGCGCCACTGCCGCTAGGGGCCTGCGGCGCCTACACCACGGCGATCCTGCAGCACCAGGCGGCCCT
GCTGGCGGCGGCACAGGGCCCAGGCCTAGGCCCGGTGGCGGCAGTGGCGGCCCAGATGCAACACGTGGCG
GCCTTTAGCCTGGTAGCTGCGCCTCTGTTGCCCGCGGCAGCAGCCAACTCCCCGCCTGGCAGCGGCCCTG
GCACCCTCCCAGGTCTTCCGGCGCCCATCGGGGTCAATGGATTCGGCCCTCTGACCCCCCAGACCAATGG
CCAGCCGGGCTCCGACACGCTCTACAATAACGGGCTCTCCCCTTATCCAGCCCAGAGCCCCGGCGTGGCT
GACCCCCTGCAGCAGGCCTACGCTGGGATGCACCACTACGCAGCAGCCTATCCGTCGGCCTATGCCCCAG
TGAGCACAGCTTTTCCCCAGCAGCCTTCAGCCCTGCCCCAGCAGCAGAGAGAAGGCCCCGAAGGCTGTAA
CCTCTTCATCTATCACCTGCCTCAGGAGTTTGGTGATGCGGAACTCATACAGACATTCCTGCCCTTTGGA
GCCGTTGTCTCTGCTAAAGTCTTTGTGGATCGAGCCACCAACCAGAGCAAGTGTTTTGGGTTTGTTAGTT
TTGACAATCCAACTAGTGCCCAGACTGCTATTCAGGCGATGAATGGCTTTCAAATTGGCATGAAGAGGCT
CAAGGCCCAGCTAAAGCGGCCCAAGGATGCCAACCGGCCTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205606 protein sequence
Red=Cloning site Green=Tags(s)

MTAAPGGSAQPAGPGPRLGFSTADSGVGMSGLNPGPAVPMKDHDAIKLFVGQIPRGLDEQDLKPLFEEFG
RIYELTVLKDRLTGLHKGCAFLTYCARDSALKAQSALHEQKTLPGMNRPIQVKPAASEGRGEDRKLFVGM
LGKQQGEEDVRRLFQPFGHIEECTVLRSPDGTSKGCAFVKFGSQGEAQAAIRGLHGSRTMAGASSSLVVK
LADTDRERALRRMQQMAGHLGAFHPAPLPLGACGAYTTAILQHQAALLAAAQGPGLGPVAAVAAQMQHVA
AFSLVAAPLLPAAAANSPPGSGPGTLPGLPAPIGVNGFGPLTPQTNGQPGSDTLYNNGLSPYPAQSPGVA
DPLQQAYAGMHHYAAAYPSAYAPVSTAFPQQPSALPQQQREGPEGCNLFIYHLPQEFGDAELIQTFLPFG
AVVSAKVFVDRATNQSKCFGFVSFDNPTSAQTAIQAMNGFQIGMKRLKAQLKRPKDANRPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_052840
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052840.2
RefSeq Size 3418 bp
RefSeq ORF 1446 bp
Locus ID 60677
UniProt ID Q96J87
MW 50.5 kDa
Gene Summary Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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