ANKRD1 (NM_014391) Human Tagged ORF Clone
CAT#: RC205609
ANKRD1 (Myc-DDK-tagged)-Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1)
ORF Plasmid: tGFP
"NM_014391" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALRP; bA320F15.2; C-193; CARP; CVARP; MCARP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205609 representing NM_014391
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGTACTGAAAGTAGAGGAACTGGTCACTGGAAAGAAGAATGGCAATGGGGAGGCAGGGGAATTCC TTCCTGAGGATTTCAGAGATGGAGAGTATGAAGCTGCTGTTACTTTAGAGAAGCAGGAGGATCTGAAGAC ACTTCTAGCCCACCCTGTGACCCTGGGGGAGCAACAGTGGAAAAGCGAGAAACAACGAGAGGCAGAGCTC AAAAAGAAAAAACTAGAACAAAGATCAAAGCTTGAAAATTTAGAAGACCTTGAAATAATCATTCAACTGA AGAAAAGGAAAAAATACAGGAAAACTAAAGTTCCAGTTGTAAAGGAACCAGAACCTGAAATCATTACGGA ACCTGTGGATGTGCCTACGTTTCTGAAGGCTGCTCTGGAGAATAAACTGCCAGTAGTAGAAAAATTCTTG TCAGACAAGAACAATCCAGATGTTTGTGATGAGTATAAACGGACAGCTCTTCATAGAGCATGCTTGGAAG GACATTTGGCAATTGTGGAGAAGTTAATGGAAGCTGGAGCCCAGATCGAATTCCGTGATATGCTTGAATC CACAGCCATCCACTGGGCAAGCCGTGGAGGAAACCTGGATGTTTTAAAATTGTTGCTGAATAAAGGAGCA AAAATTAGCGCCCGAGATAAGTTGCTCAGCACAGCGCTGCATGTGGCGGTGAGGACTGGCCACTATGAGT GCGCGGAGCATCTTATCGCCTGTGAGGCAGACCTCAACGCCAAAGACAGAGAAGGAGATACCCCGTTGCA TGATGCGGTGAGACTGAACCGCTATAAGATGATCCGACTCCTGATTATGTATGGCGCGGATCTCAACATC AAGAACTGTGCTGGGAAGACGCCGATGGATCTGGTGCTACACTGGCAGAATGGAACCAAAGCAATATTCG ACAGCCTCAGAGAGAACTCCTACAAGACCTCTCGCATAGCTACATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205609 representing NM_014391
Red=Cloning site Green=Tags(s) MMVLKVEELVTGKKNGNGEAGEFLPEDFRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAEL KKKKLEQRSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKAALENKLPVVEKFL SDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWASRGGNLDVLKLLLNKGA KISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGADLNI KNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014391 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014391.3 |
RefSeq Size | 1994 bp |
RefSeq ORF | 960 bp |
Locus ID | 27063 |
UniProt ID | Q15327 |
MW | 36.1 kDa |
Gene Summary | The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system. [provided by RefSeq, Jul 2008] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
,Bin, L;Li, X;Richers, B;Streib, JE;Hu, JW;Taylor, P;Leung, DYM;,
J. Allergy Clin. Immunol.
,PubMed ID 29371118
[ANKRD1]
|
ANKRD1 modulates inflammatory responses IN C2C12 myoblasts through feedback inhibition OF NF-?B signaling activity
,Liu, XH;Bauman, WA;Cardozo, C;,
Biochem. Biophys. Res. Commun.
,PubMed ID 26102030
[ANKRD1]
|
Inhibition of ANKRD1 sensitizes human ovarian cancer cells to endoplasmic reticulum stress-induced apoptosis
,Lei, Y;Henderson, BR;Emmanuel, C;Harnett, PR;Defazio, A;,
Oncogene, Feb 2014.
,PubMed ID 24531715
[ANKRD1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205609L1 | Lenti ORF clone of Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC205609L2 | Lenti ORF clone of Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1), mGFP tagged |
CNY 5,890.00 |
|
RC205609L3 | Lenti ORF clone of Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC205609L4 | Lenti ORF clone of Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1), mGFP tagged |
CNY 4,800.00 |
|
RG205609 | ANKRD1 (tGFP-tagged) - Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1) |
CNY 4,000.00 |
|
SC304093 | ANKRD1 (untagged)-Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1) |
CNY 6,270.00 |
|
SC321099 | ANKRD1 (untagged)-Human ankyrin repeat domain 1 (cardiac muscle) (ANKRD1) |
CNY 2,400.00 |