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C20orf11 (GID8) (NM_017896) Human Tagged ORF Clone
CAT＃: RC205664
GID8 (Myc-DDK-tagged)-Human chromosome 20 open reading frame 11 (C20orf11)
ORF Plasmid: tGFP
"NM_017896" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C20orf11; TWA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205664 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTATGCAGAAAAACCCGATGAAATCACGAAAGATGAGTGGATGGAAAAGCTCAATAACTTGCATG TCCAGAGAGCAGACATGAACCGCCTCATCATGAACTACCTGGTCACAGAGGGCTTTAAGGAAGCAGCGGA GAAGTTTCGAATGGAATCTGGAATCGAACCTAGTTTGGATCTGGAAACACTTGATGAACGAATCAAGATC CGGGAGATGATACTGAAAGGTCAGATTCAGGAGGCCATCGCCTTGATCAACAGCCTCCACCCAGAGCTCT TGGACACAAACCGGTATCTTTACTTCCATTTGCAGCAACAGCATTTGATCGAGCTGATCCGCCAGCGGGA GACAGAGGCGGCGCTGGAGTTTGCACAGACTCAGCTGGCGGAGCAGGGCGAGGAGAGCCGAGAGTGCCTC ACAGAGATGGAGCGTACCCTGGCACTGCTGGCCTTTGACAGTCCCGAGGAGTCGCCCTTCGGAGACCTCC TCCACACCATGCAGAGGCAGAAGGTGTGGAGTGAAGTTAACCAAGCTGTGCTAGATTATGAAAATCGCGA GTCAACACCCAAACTGGCAAAATTACTGAAACTACTACTTTGGGCTCAGAACGAGCTGGACCAGAAGAAA GTAAAATATCCCAAAATGACAGACCTCAGCAAGGGTGTGATTGAGGAGCCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205664 protein sequence
Red=Cloning site Green=Tags(s) MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSLDLETLDERIKI REMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECL TEMERTLALLAFDSPEESPFGDLLHTMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKK VKYPKMTDLSKGVIEEPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017896 |
| ORF Size | 684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017896.3 |
| RefSeq Size | 4440 bp |
| RefSeq ORF | 687 bp |
| Locus ID | 54994 |
| UniProt ID | Q9NWU2 |
| Domains | LisH, CTLH |
| MW | 26.8 kDa |
| Gene Summary | Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (PubMed:28829046).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205664L3 | Lenti ORF clone of Human chromosome 20 open reading frame 11 (C20orf11), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205664L4 | Lenti ORF clone of Human chromosome 20 open reading frame 11 (C20orf11), mGFP tagged |
CNY 5,890.00 |
|
| RG205664 | GID8 (tGFP-tagged) - Human chromosome 20 open reading frame 11 (C20orf11) |
CNY 4,000.00 |
|
| SC319568 | GID8 (untagged)-Human chromosome 20 open reading frame 11 (C20orf11) |
CNY 2,400.00 |
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