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PCID1 (EIF3M) (NM_006360) Human Tagged ORF Clone
CAT＃: RC205694
EIF3M (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M)
ORF Plasmid: tGFP
"NM_006360" in other vectors (7)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B5; GA17; hfl-B5; PCID1; TANGO7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205694 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTCCCGGCCTTCATCGACATCAGTGAAGAAGATCAGGCTGCTGAGCTTCGTGCTTATCTGAAAT CTAAAGGAGCTGAGATTTCAGAAGAGAACTCGGAAGGTGGACTTCATGTTGATTTAGCTCAAATTATTGA AGCCTGTGATGTGTGTCTGAAGGAGGATGATAAAGATGTTGAAAGTGTGATGAACAGTGTGGTATCCCTA CTCTTGATCCTGGAACCAGACAAGCAAGAAGCTTTGATTGAAAGCCTATGTGAAAAGCTGGTCAAATTTC GCGAAGGTGAACGCCCGTCTCTGAGACTGCAGTTGTTAAGCAACCTTTTCCACGGGATGGATAAGAATAC TCCTGTAAGATACACAGTGTATTGCAGCCTTATTAAAGTGGCAGCATCTTGTGGGGCCATCCAGTACATC CCAACTGAGCTGGATCAAGTTAGAAAATGGATTTCTGACTGGAATCTCACCACTGAAAAAAAGCACACCC TTTTAAGACTACTTTATGAGGCACTTGTGGATTGTAAGAAGAGTGATGCTGCTTCAAAAGTCATGGTGGA ATTGCTCGGAAGTTACACAGAGGACAATGCTTCCCAGGCTCGAGTTGATGCCCACAGGTGTATTGTACGA GCATTGAAAGATCCAAATGCATTTCTTTTTGACCACCTTCTTACTTTAAAACCAGTCAAGTTTTTGGAAG GCGAGCTTATTCATGATCTTTTAACCATTTTTGTGAGTGCTAAATTGGCATCATATGTCAAGTTTTATCA GAATAATAAAGACTTCATTGATTCACTTGGCCTGTTACATGAACAGAATATGGCAAAAATGAGACTACTT ACTTTTATGGGAATGGCAGTAGAAAATAAGGAAATTTCTTTTGACACAATGCAGCAAGAACTTCAGATTG GAGCTGATGATGTTGAAGCATTTGTTATTGACGCCGTAAGAACTAAAATGGTCTACTGCAAAATTGATCA GACCCAGAGAAAAGTAGTTGTCAGTCATAGCACACATCGGACATTTGGAAAACAGCAGTGGCAACAACTG TATGACACACTTAATGCCTGGAAACAAAATCTGAACAAAGTGAAAAACAGCCTTTTGAGTCTTTCTGATA CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205694 protein sequence
Red=Cloning site Green=Tags(s) MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSL LLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYI PTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQARVDAHRCIVR ALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLL TFMGMAVENKEISFDTMQQELQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQL YDTLNAWKQNLNKVKNSLLSLSDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006360 |
| ORF Size | 1122 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006360.6 |
| RefSeq Size | 1338 bp |
| RefSeq ORF | 1125 bp |
| Locus ID | 10480 |
| UniProt ID | Q7L2H7 |
| Domains | PCI |
| MW | 42.5 kDa |
| Gene Summary | This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205694L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205694L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), mGFP tagged |
CNY 5,890.00 |
|
| RC205694L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205694L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), mGFP tagged |
CNY 5,890.00 |
|
| RG205694 | EIF3M (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit M (EIF3M) |
CNY 7,088.00 |
|
| SC116147 | EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M) |
CNY 5,488.00 |
|
| SC320414 | EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M) |
CNY 5,488.00 |
