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CALML3 (NM_005185) Human Tagged ORF Clone
CAT＃: RC205791
CALML3 (Myc-DDK-tagged)-Human calmodulin-like 3 (CALML3)
ORF Plasmid: tGFP
"NM_005185" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205791 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCAGCTGACTGAGGAGCAGGTCACAGAATTCAAGGAGGCCTTCTCCCTGTTTGACAAGGATG GGGACGGCTGCATCACCACCCGCGAGCTGGGCACGGTCATGCGGTCCCTGGGCCAGAACCCCACGGAGGC CGAGCTGCGGGACATGATGAGTGAGATCGACCGGGACGGCAACGGCACCGTGGACTTCCCCGAGTTCCTG GGCATGATGGCCAGGAAGATGAAGGACACGGACAACGAGGAGGAGATCCGCGAGGCCTTCCGCGTGTTCG ACAAGGACGGCAACGGCTTCGTCAGCGCCGCCGAGCTGCGACACGTCATGACCCGGCTGGGGGAGAAGCT GAGTGACGAGGAGGTGGACGAGATGATCCGGGCCGCGGACACGGACGGAGACGGACAGGTGAACTACGAG GAGTTTGTCCGTGTGCTGGTGTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205791 protein sequence
Red=Cloning site Green=Tags(s) MADQLTEEQVTEFKEAFSLFDKDGDGCITTRELGTVMRSLGQNPTEAELRDMMSEIDRDGNGTVDFPEFL GMMARKMKDTDNEEEIREAFRVFDKDGNGFVSAAELRHVMTRLGEKLSDEEVDEMIRAADTDGDGQVNYE EFVRVLVSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005185 |
| ORF Size | 447 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005185.4 |
| RefSeq Size | 1417 bp |
| RefSeq ORF | 450 bp |
| Locus ID | 810 |
| UniProt ID | P27482 |
| Protein Pathways | Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction |
| MW | 16.9 kDa |
| Gene Summary | May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205791L1 | Lenti ORF clone of Human calmodulin-like 3 (CALML3), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC205791L2 | Lenti ORF clone of Human calmodulin-like 3 (CALML3), mGFP tagged |
CNY 5,890.00 |
|
| RC205791L3 | Lenti ORF clone of Human calmodulin-like 3 (CALML3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205791L4 | Lenti ORF clone of Human calmodulin-like 3 (CALML3), mGFP tagged |
CNY 5,890.00 |
|
| RG205791 | CALML3 (tGFP-tagged) - Human calmodulin-like 3 (CALML3) |
CNY 2,800.00 |
|
| SC122701 | CALML3 (untagged)-Human calmodulin-like 3 (CALML3) |
CNY 3,990.00 |
