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ATG4C (NM_178221) Human Tagged ORF Clone
CAT＃: RC205955
ATG4C (Myc-DDK-tagged)-Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2
ORF Plasmid: tGFP
"NM_178221" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APG4-C; APG4C; AUTL1; AUTL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205955 representing NM_178221
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTACAGGAACAGATGAAGTTGACAAGCTAAAAACCAAATTTATATCTGCTTGGAACAACATGA AATATAGTTGGGTGTTGAAAACAAAGACGTATTTTAGTAGAAATTCTCCTGTATTATTGCTTGGAAAATG TTACCATTTTAAATATGAAGATGAAGATAAAACGTTACCTGCAGAGTCGGGATGTACAATAGAGGATCAC GTAATTGCAGGAAATGTAGAAGAATTTCGTAAAGATTTCATTTCTAGAATATGGCTGACCTACAGGGAAG AATTCCCTCAAATAGAAGGCTCAGCTTTGACAACAGACTGTGGGTGGGGCTGCACATTGAGAACTGGCCA GATGCTCTTGGCTCAAGGACTCATACTACACTTTCTTGGTAGAGCTTGGACCTGGCCTGATGCTTTGAAT ATTGAAAATTCAGACTCTGAATCATGGACTTCCCACACTGTCAAAAAATTTACTGCATCATTTGAAGCAT CACTTTCAGGGGAAAGAGAATTCAAAACCCCAACAATTTCTCTGAAGGAAACAATTGGGAAATATTCTGA TGATCATGAAATGCGAAATGAAGTTTATCATAGGAAAATCATCTCTTGGTTTGGTGATTCCCCCTTGGCT CTTTTTGGCTTACATCAACTAATAGAATATGGAAAGAAGTCTGGGAAAAAAGCAGGAGATTGGTATGGAC CAGCTGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCCTGATTTACAAGGAATAAC TATTTATGTTGCACAAGATTGTACAGTTTACAATTCTGATGTAATTGATAAACAGAGTGCTTCCATGACT TCTGATAATGCAGATGACAAAGCTGTTATTATTCTAGTTCCTGTTAGACTTGGTGGAGAAAGAACCAACA CCGACTACTTAGAATTTGTGAAGGGTATTTTAAGCCTGGAATATTGTGTGGGTATTATTGGTGGCAAACC TAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATGGATCCTCATTACTGCCAA TCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTTGAGACATTCCACTGCCCTTCTCCCAAAAAGATGT CTTTTCGAAAAATGGATCCCAGCTGTACAATAGGATTTTACTGTCGAAATGTTCAGGACTTCAAACGAGC TTCTGAAGAAATCACCAAGATGCTGAAATTTTCTTCTAAGGAGAAATATCCCTTATTTACTTTTGTAAAT GGTCATTCCAGAGACTATGATTTTACATCTACTACAACCAATGAAGAAGACCTTTTTTCAGAGGATGAAA AGAAACAATTAAAAAGATTTAGCACGGAAGAGTTTGTCTTGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205955 representing NM_178221
Red=Cloning site Green=Tags(s) MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDH VIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALN IENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLA LFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMT SDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQ SFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFKRASEEITKMLKFSSKEKYPLFTFVN GHSRDYDFTSTTTNEEDLFSEDEKKQLKRFSTEEFVLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178221 |
| ORF Size | 1374 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178221.2 |
| RefSeq Size | 1774 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 84938 |
| UniProt ID | Q96DT6 |
| Protein Families | Protease |
| Protein Pathways | Regulation of autophagy |
| MW | 52.3 kDa |
| Gene Summary | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205955L1 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205955L2 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC205955L3 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205955L4 | Lenti ORF clone of Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205955 | ATG4C (tGFP-tagged) - Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2 |
CNY 5,256.00 |
|
| SC111887 | ATG4C (untagged)-Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2 |
CNY 3,656.00 |
