ACT (FHL5) (NM_020482) Human Tagged ORF Clone
CAT#: RC205990
FHL5 (Myc-DDK-tagged)-Human four and a half LIM domains 5 (FHL5), transcript variant 1
ORF Plasmid: tGFP
"NM_020482" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1700027G07Rik; ACT; dJ393D12.2; FHL-5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205990 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTGCTCACTTTTACTGTCAATACTGCACAGCATCACTTCTTGGGAAGAAATATGTACTAAAGG ATGACAGTCCATACTGTGTTACATGTTATGATCGTGTATTTTCTAACTATTGCGAGGAATGCAAAAAACC AATTGAATCTGATTCTAAGGATCTTTGTTACAAAGACCGGCACTGGCATGAAGGATGCTTCAAGTGCACC AAATGCAATCACTCTTTGGTGGAAAAGCCTTTTGCTGCCAAGGATGAGCGCCTGCTGTGCACGGAGTGCT ATTCTAACGAGTGCTCCTCCAAGTGCTTCCACTGCAAGAGGACCATCATGCCTGGTTCCCGCAAAATGGA ATTTAAGGGAAACTACTGGCATGAAACCTGTTTTGTGTGTGAGAATTGCCGACAACCTATAGGGACAAAG CCTTTGATCTCCAAAGAGAGTGGCAATTATTGTGTGCCATGTTTTGAGAAGGAGTTTGCTCACTACTGCA ACTTTTGTAAGAAGGTGATAACTTCAGGTGGGATAACATTTTGTGACCAGCTATGGCATAAAGAGTGTTT TCTGTGTAGTGACTGTAGGAAAGATCTCTGTGAAGAACAGTTCATGTCCAGAGACGACTATCCATTCTGC ATGGACTGCTACAACCATCTTTATGCCAACAAGTGTGTAGCCTGTTCCAAACCCATTAGTGGTCTCACAG GTGCCAAGTTTATCTGCTTTCAAGACAGCCAGTGGCATAGCGAATGCTTTAACTGCGGGAAATGCTCTGT CTCCTTGGTGGGTAAAGGCTTCCTGACCCAGAACAAGGAAATCTTCTGCCAAAAATGTGGCTCCGGAATG GACACTGACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205990 protein sequence
Red=Cloning site Green=Tags(s) MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCT KCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTK PLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFCDQLWHKECFLCSDCRKDLCEEQFMSRDDYPFC MDCYNHLYANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGM DTDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020482 |
ORF Size | 852 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020482.5 |
RefSeq Size | 2135 bp |
RefSeq ORF | 855 bp |
Locus ID | 9457 |
UniProt ID | Q5TD97 |
Domains | LIM |
Protein Families | Druggable Genome |
MW | 32.8 kDa |
Gene Summary | The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Apr 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205990L3 | Lenti ORF clone of Human four and a half LIM domains 5 (FHL5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205990L4 | Lenti ORF clone of Human four and a half LIM domains 5 (FHL5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG205990 | FHL5 (tGFP-tagged) - Human four and a half LIM domains 5 (FHL5), transcript variant 1 |
CNY 4,000.00 |
|
SC113089 | FHL5 (untagged)-Human four and a half LIM domains 5 (FHL5), transcript variant 1 |
CNY 2,400.00 |