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Cytohesin 3 (CYTH3) (NM_004227) Human Tagged ORF Clone
CAT＃: RC206003
CYTH3 (Myc-DDK-tagged)-Human cytohesin 3 (CYTH3)
ORF Plasmid: tGFP
"NM_004227" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARNO3; cytohesin-3; GRP1; PSCD3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206003 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAAGACGGCGGCGGCGAGGGTGGTGGCGTGCCTGAAGACCTCTCATTAGAAGAGAGAGAAGAAC TTCTAGACATTCGTCGAAGAAAAAAGGAACTTATTGATGACATTGAGAGGCTGAAATATGAAATTGCAGA GGTGATGACAGAGATCGACAATCTAACTTCCGTAGAGGAGAGCAAAACGACTCAGAGGAACAAACAGATA GCCATGGGAAGAAAGAAATTCAACATGGATCCCAAAAAGGGAATTCAGTTTCTAATAGAAAATGACCTGC TACAGAGTTCCCCAGAAGACGTCGCCCAGTTCCTTTATAAAGGAGAAGGCCTAAATAAGACCGTCATTGG GGACTACCTGGGTGAAAGGGATGAATTTAATATTAAAGTTCTTCAAGCCTTTGTTGAACTCCATGAGTTT GCTGATCTCAACCTTGTACAAGCCTTAAGGCAGTTCTTATGGAGCTTCAGGCTGCCCGGGGAGGCGCAGA AGATTGATCGCATGATGGAGGCTTTCGCTTCTCGCTACTGCCTGTGCAACCCCGGGGTCTTCCAGTCCAC AGACACGTGCTACGTGCTGTCATTCGCCATCATCATGCTCAACACCAGCCTCCACAACCACAACGTGCGT GACAAGCCCACGGCAGAACGGTTCATCGCCATGAACCGCGGCATCAACGAGGGCGGGGACCTCCCTGAGG AGCTGCTGAGGAATTTGTATGAGAGCATTAAGAACGAGCCATTTAAGATCCCGGAGGACGACGGGAACGA CCTGACCCACACCTTCTTCAACCCCGACCGCGAGGGCTGGCTCCTGAAGCTGGGAGGGCGTGTGAAGACC TGGAAGCGCCGGTGGTTCATCCTGACCGATAACTGCCTCTATTACTTTGAATACACAACAGATAAGGAGC CCAGGGGAATCATCCCGTTGGAAAACCTCAGCATCAGGGAGGTGGAGGACCCCCGGAAACCCAACTGTTT TGAGCTCTACAATCCCAGCCACAAAGGGCAGGTCATCAAGGCCTGTAAGACGGAGGCCGACGGCCGCGTG GTAGAGGGGAACCATGTGGTGTACCGGATCTCAGCCCCGAGCCCGGAGGAGAAGGAGGAGTGGATGAAAT CCATCAAAGCCAGTATCAGCAGAGATCCCTTCTATGACATGTTGGCAACGAGGAAACGAAGGATTGCCAA TAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206003 protein sequence
Red=Cloning site Green=Tags(s) MDEDGGGEGGGVPEDLSLEEREELLDIRRRKKELIDDIERLKYEIAEVMTEIDNLTSVEESKTTQRNKQI AMGRKKFNMDPKKGIQFLIENDLLQSSPEDVAQFLYKGEGLNKTVIGDYLGERDEFNIKVLQAFVELHEF ADLNLVQALRQFLWSFRLPGEAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVR DKPTAERFIAMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKT WKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRV VEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004227 |
| ORF Size | 1197 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004227.2 |
| RefSeq Size | 4482 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 9265 |
| UniProt ID | O43739 |
| Domains | Sec7, PH |
| Protein Families | Druggable Genome |
| MW | 46.3 kDa |
| Gene Summary | This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206003L3 | Lenti ORF clone of Human cytohesin 3 (CYTH3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206003L4 | Lenti ORF clone of Human cytohesin 3 (CYTH3), mGFP tagged |
CNY 5,890.00 |
|
| RG206003 | CYTH3 (tGFP-tagged) - Human cytohesin 3 (CYTH3) |
CNY 7,088.00 |
|
| SC127412 | CYTH3 (untagged)-Human cytohesin 3 (CYTH3) |
CNY 3,656.00 |
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