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MEK4 (MAP2K4) (NM_003010) Human Tagged ORF Clone
CAT＃: RC206051
MAP2K4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 4 (MAP2K4)
ORF Plasmid: tGFP
"NM_003010" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
| Cited in 4 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206051 representing NM_003010
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCCGAGCCCGAGCGGCGGCGGCGGCTCCGGGGGCGGCCGCGGCAGCGGCACCCCCGGCCCCG TAGGGTCCCCGGCGCCAGGCCACCCGGCCGTCAGCAGCATGCAGGGTAAACGCAAAGCACTGAAGTTGAA TTTTGCAAATCCACCTTTCAAATCTACAGCAAGGTTTACTCTGAATCCCAATCCTACAGGAGTTCAAAAC CCACACATAGAGAGACTGAGAACACACAGCATTGAGTCATCAGGAAAACTGAAGATCTCCCCTGAACAAC ACTGGGATTTCACTGCAGAGGACTTGAAAGACCTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCAA CAAAATGGTCCACAAACCAAGTGGGCAAATAATGGCAGTTAAAAGAATTCGGTCAACAGTGGATGAAAAA GAACAAAAACAACTTCTTATGGATTTGGATGTAGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGT TTTATGGTGCACTCTTCAGAGAGGGTGACTGTTGGATCTGTATGGAACTCATGTCTACCTCGTTTGATAA GTTTTACAAATATGTATATAGTGTATTAGATGATGTTATTCCAGAAGAAATTTTAGGCAAAATCACTTTA GCAACTGTGAAAGCACTAAACCACTTAAAAGAAAACTTGAAAATTATTCACAGAGATATCAAACCTTCCA ATATTCTTCTGGACAGAAGTGGAAATATTAAGCTCTGTGACTTCGGCATCAGTGGACAGCTTGTGGACTC TATTGCCAAGACAAGAGATGCTGGCTGTAGGCCATACATGGCACCTGAAAGAATAGACCCAAGCGCATCA CGACAAGGATATGATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTATGAGTTGGCCACAGGCC GATTTCCTTATCCAAAGTGGAATAGTGTATTTGATCAACTAACACAAGTCGTGAAAGGAGATCCTCCGCA GCTGAGTAATTCTGAGGAAAGGGAATTCTCCCCGAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAG GATGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTTATTTTGATGTATGAAGAACGTG CCGTTGAGGTCGCATGCTATGTTTGTAAAATCCTGGATCAAATGCCAGCTACTCCCAGCTCTCCCATGTA TGTCGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206051 representing NM_003010
Red=Cloning site Green=Tags(s) MAAPSPSGGGGSGGGRGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQN PHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEK EQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITL ATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSAS RQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTK DESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003010 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003010.4 |
| RefSeq Size | 3752 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 6416 |
| UniProt ID | P45985 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway |
| MW | 44.1 kDa |
| Gene Summary | This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Truncation- and motif-based pan-cancer analysis reveals tumor-suppressing kinases
,Hudson, AM;Stephenson, NL;Li, C;Trotter, E;Fletcher, AJ;Katona, G;Bieniasz-Krzywiec, P;Howell, M;Wirth, C;Furney, S;Miller, CJ;Brognard, J;,
Sci Signal
,PubMed ID 29666306
[MEK4]
|
|
Truncation and Motif Based Pan-Cancer Analysis Highlights Novel Tumor Suppressing Kinases
,Hudson, A;Stepehnson, N;Li, C;Trotter, E;Fletcher, A;Katona, G;Bieniasz-Krzywiec, P;Howell, M;Wirth, C;Miller, C;Brognard, J;,
bioRxiv
[MEK4]
|
|
Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers
,null,
eLife
,PubMed ID 26894955
[MEK4]
|
|
Diverse somatic mutation patterns and pathway alterations in human cancers
,Zhengyan Kan, Bijay S. Jaiswal, Jeremy Stinson, Vasantharajan Janakiraman, Deepali Bhatt, Howard M. Stern, Peng Yue, Peter M. Haverty, Richard Bourgon, Jianbiao Zheng, et al.,
Nature (28 July 2010) doi:10.1038/nature09208 Letter
[MEK4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206051L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 4 (MAP2K4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206051L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 4 (MAP2K4), mGFP tagged |
CNY 5,890.00 |
|
| RC206051L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 4 (MAP2K4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206051L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 4 (MAP2K4), mGFP tagged |
CNY 6,056.00 |
|
| RG206051 | MAP2K4 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 4 (MAP2K4) |
CNY 5,256.00 |
|
| SC118240 | MAP2K4 (untagged)-Human mitogen-activated protein kinase kinase 4 (MAP2K4) |
CNY 3,656.00 |
|
| SC323476 | MAP2K4 (untagged)-Kinase deficient mutant (K131M) of Human mitogen-activated protein kinase kinase 4 (MAP2K4) |
CNY 3,656.00 |
