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SPG7 (NM_003119) Human Tagged ORF Clone
CAT＃: RC206340
SPG7 (Myc-DDK-tagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_003119" in other vectors (6)
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CNY 8,736.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAR; CMAR; PGN; SPG5C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206340 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGCTGCTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGG GCCCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATGGCCAGCAG GCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAATTGAGACTGCTAACCCCT ACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTAGTTCAGAATCCAGTCAGACTCTGGCAAC TTTTAGGTGGTACTTTCTATTTTAACACCTCAAGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAA GGGGAAGGCGCCTGAAGAGGACGAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAG CGGCTGCGCACCTTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCA GCATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAGGTGGTGCC TGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCCGTGGTGTTTGGGCGGCCTCGGCTAGCCTTG ATGTACCGAATGCAGGTTGCAAATATTGACAAGTTTGAAGAGAAGCTTCGAGCAGCTGAAGATGAGCTGA ATATCGAGGCCAAGGACAGGATCCCAGTTTCCTACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTC TGTGGGGATGACGGCAGTGGGCCTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGG GAAGGTGGATTCAGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGGAAGATGGGGA AAGGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAAGTCCGCGAGTTTGTGGATTA TCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAGGTCCCAAAGGGCGCACTGCTGCTCGGCCCC CCCGGCTGTGGGAAGACGCTGCTGGCCAAGGCGGTGGCCACGGAGGCTCAGGTGCCCTTCCTGGCGATGG CCGGCGCAGAGTTCGTGGAGGTCATTGGAGGCCTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGC CCGAGCCCGGGCCCCCTGCATCGTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACC ATGTCCGGCTTCTCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATGGATGGAATGG GTACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATTTTGGACGGTGCTCTGATGAG GCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCCACGCTGCAGGAGAGGCGGGAGATTTTTGAG CAGCACCTGAAGAGCCTGAAGCTGACCCAGTCCAGCACCTTTTACTCCCAGCGTCTGGCAGAGCTGACAC CAGGATTCAGTGGGGCTGACATCGCCAACATCTGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACA CACTTCCGTGCACACTCTCAACTTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGT AAGATCCTGTCCAAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTGGTGGGCTGGA TGCTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACAAACGCCGCCCTGGGCTTTGC TCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAGCAGCTGTTTGAGCGGATGTGCATGGCCCTG GGAGGACGGGCCTCGGAAGCACTGTCCTTCAACGAGGTCACTTCTGGGGCACAGGACGACCTGAGGAAGG TCACCCGCATCGCCTACTCCATGGTGAAGCAGTTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCC TGAGGCGCAGGAGGGCCTCATGGGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGAC CATGAAGCAAGACTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGACAACCTGGACA AGTTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAGGCTCTCAT TGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCGCAGAGGTGGATCGACGCCCAGAGGGAGAAA CAGGACTTGGGCGAGGAGGAGACCGAAGAGACCCAGCAGCCTCCACTTGGAGGCGAAGAGCCGACTTGGC CCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206340 protein sequence
Red=Cloning site Green=Tags(s) MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTP TFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRE RLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLAL MYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGR EGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGP PGCGKTLLAKAVATEAQVPFLAMAGAEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTT MSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADILDGALMRPGRLDRHVFIDLPTLQERREIFE QHLKSLKLTQSSTFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTLNFEYAVERVLAGTAKKS KILSKEEQKVVAFHESGHALVGWMLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTKEQLFERMCMAL GGRASEALSFNEVTSGAQDDLRKVTRIAYSMVKQFGMAPGIGPISFPEAQEGLMGIGRRPFSQGLQQMMD HEARLLVAKAYRHTEKVLQDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQRWIDAQREK QDLGEEETEETQQPPLGGEEPTWPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003119 |
| ORF Size | 2385 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003119.4 |
| RefSeq Size | 3102 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 6687 |
| UniProt ID | Q9UQ90 |
| Domains | Peptidase_M41, AAA, AAA |
| Protein Families | Protease, Transmembrane |
| MW | 88.2 kDa |
| Gene Summary | This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206340L1 | Lenti ORF clone of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 11,136.00 |
|
| RC206340L2 | Lenti ORF clone of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206340L3 | Lenti ORF clone of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 11,136.00 |
|
| RC206340L4 | Lenti ORF clone of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 11,136.00 |
|
| RG206340 | SPG7 (tGFP-tagged) - Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 10,336.00 |
|
| SC126532 | SPG7 (untagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,744.00 |
