RGS3 (NM_134427) Human Tagged ORF Clone
CAT#: RC206471
- TrueORF®
RGS3 (Myc-DDK-tagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 4
ORF Plasmid: tGFP
"NM_134427" in other vectors (4)
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CNY 6,720.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C2PA; RGP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGCTTCAATGGGCTCTGCAAGGTGTGCTCGGAGCGCCGCTACCGCCAGATCACCATCCCGAGGG GAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGAGTCCAGGCCGTGGATTCCGGGGG TCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGCTGAATGAGAGGCCTGTGGAGCAC TGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGAGATCATCCTACTCGTGTGGCGCA TGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTCCTGCGGCGGGCCTCCTGCAAGTCGACACATGA CCTCCAGTCACCCCCCAACAAACGGGAGAAGAACTGCACCCATGGGGTCCAGGCACGGCCTGAGCAGCGC CACAGCTGCCACCTGGTATGTGACAGCTCTGATGGGCTGCTGCTCGGCGGCTGGGAGCGCTACACCGAGG TGGCCAAGCGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCCACTGCCCCCACCGACCCCAACTA CATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTGTGTACCAGGAGGATACCATCCCC GAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGGGAAGAAGTCCCGGCTGATGAAGA CAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAACTGCCCGGTTGTGAGGCCGCATGCCACGCACTC AAGCTATGGCACCTACGTCACCCTGGCCCCCAAAGTCCTGGTGTTCCCTGTCTTTGTTCAGCCTCTAGAT CTCTGTAATCCTGCCCGGACCCTCCTGCTGTCAGAGGAGCTGCTGCTGTATGAAGGGAGGAACAAGGCTG CCGAGGTGACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAGGAGGACGAGCCTGGCCGCTGCGA CGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAGGTTCTTCAGAAGACCTGAAATTC TGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAAGCGCACTCGCAGGAGCAGAAGA AGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAGCTGGCAGCATCACCCCCGGACAG CAAGATGTTTGAGACGGAGGCAGATGAGAAGAGGGAGATGGCCTTGGAGGAAGGGAAGGGGCCTGGTGCC GAGGATTCCCCACCCAGCAAGGAGCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTTCCACCCA GCAAGGACTCCCCTTCTGGGCAGGAACCCGCTCCCAGCCAAGAACCACTGTCCAGCAAAGACTCAGCTAC CTCTGAAGGATCCCCTCCAGGCCCAGATGCTCCGCCCAGCAAGGATGTGCCACCATGCCAGGAACCCCCT CCAGCCCAAGACCTCTCACCCTGCCAGGACCTACCTGCTGGTCAAGAACCCCTGCCTCACCAGGACCCTC TACTCACCAAAGACCTCCCTGCCATCCAGGAATCCCCCACCCGGGACCTTCCACCCTGTCAAGATCTGCC TCCTAGCCAGGTCTCCCTGCCAGCCAAGGCCCTTACTGAGGACACCATGAGCTCCGGGGACCTACTAGCA GCTACTGGGGACCCACCTGCGGCCCCCAGGCCAGCCTTCGTGATCCCTGAGGTCCGGCTGGATAGCACCT ACAGCCAGAAGGCAGGGGCAGAGCAGGGCTGCTCGGGAGATGAGGAGGATGCAGAAGAGGCCGAGGAGGT GGAGGAGGGGGAGGAAGGGGAGGAGGACGAGGATGAGGACACCAGCGATGACAACTACGGAGAGCGCAGT GAGGCCAAGCGCAGCAGCATGATCGAGACGGGCCAGGGGGCTGAGGGTGGCCTCTCACTGCGTGTGCAGA ACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAGCCCCGAGGGCCCTGCTTTGCCTC CGACACCACCTTGCACTGCTCAGACGGTGAGGGCGCCGCCTCCACCTGGGGCATGCCTTCGCCCAGCACC CTCAAGAAAGAGCTGGGCCGCAATGGTGGCTCCATGCACCACCTTTCCCTCTTCTTCACAGGACACAGGA AGATGAGCGGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGGAAGAGAAAGAGCAAAAACCTAGC CAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCCCCTCCCGCGGGC AAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCCCTCAAGTGGGGCGAGTCCTTGG AGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTCCTTCGCACTGAGTTCAGTGAGGA GAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCAAGTCACAGTCCAAGATGGCATCCAAG GCCAAGAAGATCTTTGCTGAATACATCGCGATCCAGGCATGCAAGGAGGTCAACCTGGACTCCTACACGC GGGAGCACACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTCGACCTGGCACAGAAGCGCATCTT CGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACCTCTACCTGGACCTTATTAACCAG AAGAAGATGAGTCCCCCGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206471 protein sequence
Red=Cloning site Green=Tags(s) MNRFNGLCKVCSERRYRQITIPRGKDGFGFTICCDSPVRVQAVDSGGPAERAGLQQLDTVLQLNERPVEH WKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRRASCKSTHDLQSPPNKREKNCTHGVQARPEQR HSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRATAPTDPNYIILAPLNPGSQLLRPVYQEDTIP EESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPVVRPHATHSSYGTYVTLAPKVLVFPVFVQPLD LCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTKEDEPGRCDVLRNPLYLQSVKLQEGSSEDLKF CVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAASPPDSKMFETEADEKREMALEEGKGPGA EDSPPSKEPSPGQELPPGQDLPPSKDSPSGQEPAPSQEPLSSKDSATSEGSPPGPDAPPSKDVPPCQEPP PAQDLSPCQDLPAGQEPLPHQDPLLTKDLPAIQESPTRDLPPCQDLPPSQVSLPAKALTEDTMSSGDLLA ATGDPPAAPRPAFVIPEVRLDSTYSQKAGAEQGCSGDEEDAEEAEEVEEGEEGEEDEDEDTSDDNYGERS EAKRSSMIETGQGAEGGLSLRVQNSLRRRTHSEGSLLQEPRGPCFASDTTLHCSDGEGAASTWGMPSPST LKKELGRNGGSMHHLSLFFTGHRKMSGADTVGDDDEASRKRKSKNLAKDMKNKLGIFRRRNESPGAPPAG KADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMASK AKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLINQ KKMSPPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134427 |
ORF Size | 2754 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1733 bp |
RefSeq ORF | 507 bp |
Locus ID | 5998 |
UniProt ID | P49796 |
Domains | RGS |
Protein Families | Druggable Genome |
Protein Pathways | Axon guidance |
MW | 100.9 kDa |
Gene Summary | This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206471L3 | Lenti-ORF clone of RGS3 (Myc-DDK-tagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 4 |
CNY 5,890.00 |
|
RC206471L4 | Lenti-ORF clone of RGS3 (mGFP-tagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 4 |
CNY 5,890.00 |
|
RG206471 | RGS3 (tGFP-tagged) - Human regulator of G-protein signaling 3 (RGS3), transcript variant 4 |
CNY 8,320.00 |
|
SC109729 | RGS3 (untagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 4 |
CNY 2,400.00 |