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GPR182 (NM_007264) Human Tagged ORF Clone
CAT＃: RC206505
GPR182 (Myc-DDK-tagged)-Human G protein-coupled receptor 182 (GPR182)
ORF Plasmid: tGFP
"NM_007264" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 7TMR; ADMR; AM-R; AMR; G10D; gamrh; hrhAMR; L1-R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206505 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACCAGTGACCTTG GAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTGTCTGAGTGCCACGTGGAGCT CAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGGGCTGGTGGAG AACCTCCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTCTACATCCTCA ACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCACGCTGGACTA CACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACATGTATAGCAGC ATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCCTCCTGGCAGC GTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCATCCCGCTGCC TGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACCTTTTGAAACG TACAGCACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCCTTCCCTCTCA TCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCCGGCGCCACTG CCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGACCCTGCTGCTG CTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTCTTCTATGATG TCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTACAACTTTCTCAGCCCACACTT CCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGGCACATGCGCC TCCTCTTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCTGCTGCAGCAG CCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCCCCATCTCTCC CACTCAGCCTCTTACACCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206505 protein sequence
Red=Cloning site Green=Tags(s) MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALYLAMFVVGLVE NLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWLWGSFSCRFTHYFYFVNMYSS IFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVLSAIIPLPEVVHIQLVEGPEPMCLFMAPFET YSTWALAVALSTTILGFLLPFPLITVFNVLTACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLL LTLHGTHISLHCHLVHLLYFFYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCA SSSSCSTQHSIIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007264 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007264.4 |
| RefSeq Size | 1620 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 11318 |
| UniProt ID | O15218 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 45.3 kDa |
| Gene Summary | Adrenomedullin is a potent vasodilator peptide that exerts major effects on cardiovascular function. This gene encodes a seven-transmembrane protein that belongs to the family 1 of G-protein coupled receptors. Studies of the rat counterpart suggest that the encoded protein may function as a receptor for adrenomedullin. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206505L1 | Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206505L2 | Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), mGFP tagged |
CNY 5,890.00 |
|
| RC206505L3 | Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206505L4 | Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), mGFP tagged |
CNY 5,890.00 |
|
| RG206505 | GPR182 (tGFP-tagged) - Human G protein-coupled receptor 182 (GPR182) |
CNY 5,256.00 |
|
| SC115624 | GPR182 (untagged)-Human G protein-coupled receptor 182 (GPR182) |
CNY 3,656.00 |
