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Calcineurin B (PPP3R1) (NM_000945) Human Tagged ORF Clone
CAT＃: RC206585
PPP3R1 (Myc-DDK-tagged)-Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1)
ORF Plasmid: tGFP
"NM_000945" in other vectors (5)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CALNB1; CNB; CNB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206585 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATGAGGCAAGTTATCCTTTGGAAATGTGCTCACACTTTGATGCGGATGAAATTAAAAGGCTAG GAAAGAGATTTAAGAAGCTTGATTTGGACAATTCTGGTTCTTTGAGTGTGGAAGAGTTCATGTCTCTGCC TGAGTTACAACAGAATCCTTTAGTACAGCGAGTAATAGATATATTCGACACAGATGGGAATGGAGAAGTA GACTTTAAAGAATTCATTGAGGGCGTCTCTCAGTTCAGTGTCAAAGGAGATAAGGAGCAGAAATTGAGGT TTGCTTTCCGTATCTATGACATGGATAAAGATGGCTATATTTCCAATGGGGAACTCTTCCAGGTATTGAA GATGATGGTGGGGAACAATCTGAAAGATACACAGTTACAGCAAATTGTAGACAAAACCATAATAAATGCA GATAAGGATGGAGATGGAAGAATATCCTTTGAAGAATTCTGTGCTGTTGTAGGTGGCCTAGATATCCACA AAAAGATGGTGGTAGATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206585 protein sequence
Red=Cloning site Green=Tags(s) MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEV DFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINA DKDGDGRISFEEFCAVVGGLDIHKKMVVDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000945 |
| ORF Size | 510 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000945.4 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 513 bp |
| Locus ID | 5534 |
| UniProt ID | P63098 |
| Domains | EFh |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| MW | 19.3 kDa |
| Gene Summary | Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206585L3 | Lenti ORF clone of Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206585L4 | Lenti ORF clone of Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1), mGFP tagged |
CNY 5,890.00 |
|
| RG206585 | PPP3R1 (tGFP-tagged) - Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1) |
CNY 4,000.00 |
|
| SC127998 | PPP3R1 (untagged)-Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1) |
CNY 3,990.00 |
|
| SC322580 | PPP3R1 (untagged)-Human protein phosphatase 3, regulatory subunit B, alpha (PPP3R1) |
CNY 2,400.00 |
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