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PDE1B (NM_000924) Human Tagged ORF Clone
CAT＃: RC206588
PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1
ORF Plasmid: tGFP
"NM_000924" in other vectors (6)
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CNY 3,992.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-79p; PDE1B1; PDES1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206588 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTCCCCCCGCAGTCCTCCGGAGATGCTGGAGGAGTCGGATTGCCCGTCACCCCTGGAGCTGA AGTCAGCCCCCAGCAAGAAGATGTGGATTAAGCTTCGGTCTCTGCTGCGCTACATGGTGAAGCAGTTGGA GAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTTCTCTGCTGGAAGCCGTC TACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAGCTGCGGTCAGATGCCGTGC CTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCCAAAGGCCGCCGAGCAGA GGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAACGGATGTTCCGG AGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAGAACCTGGATCTCT GGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGGACCATTGTTTTTGAGTT GCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGATGAGTTTCCTGGATGCC TTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGCAGCCGATGTTACCCAGA CAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAGCTCCTGGCCATCAT CTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCACATCCAGACCAAGTCA GAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGCTCTGTTTTCCGATTGA TGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTAGAACTCCGAGCCCTGGT CATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACCATGAAGACAGCCTTG CAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCATGCTGCTGACATCAGCCACC CAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAATTCTTCCGTCAGGGTGA CAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCACTCTAGTGGCACAGTCT CAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGACGTGGCAGAGAAGAGTG TTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGCTTTCAGTGGCGCCAGCCCTCTCT GGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGTTCCACCTGGGTCAAGCGCATTCAG GAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGATGTCCATTGACGAGCTGT CCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAACACAACCAGAATGGGAATCTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206588 protein sequence
Red=Cloning site Green=Tags(s) MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAV YIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFR RTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDA LETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKS ECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTAL QQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQS QIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQ ENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000924 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000924.4 |
| RefSeq Size | 3463 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 5153 |
| UniProt ID | Q01064 |
| Domains | PDEase, HDc |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 61.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Preclinical profile of ITI-214, an inhibitor of phosphodiesterase 1, for enhancement of memory performance in rats
,Snyder, GL;Prickaerts, J;Wadenberg, ML;Zhang, L;Zheng, H;Yao, W;Akkerman, S;Zhu, H;Hendrick, JP;Vanover, KE;Davis, R;Li, P;Mates, S;Wennogle, LP;,
Psychopharmacology (Berl.) 2016
,PubMed ID 27342643
[PDE1B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206588L1 | Lenti ORF clone of Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, Myc-DDK-tagged |
CNY 6,392.00 |
|
| RC206588L2 | Lenti ORF clone of Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206588L3 | Lenti ORF clone of Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206588L4 | Lenti ORF clone of Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG206588 | PDE1B (tGFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1 |
CNY 5,592.00 |
|
| SC310288 | PDE1B (untagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1 |
CNY 4,000.00 |
