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Dihydropyrimidinase (DPYS) (NM_001385) Human Tagged ORF Clone
CAT＃: RC206603
DPYS (Myc-DDK-tagged)-Human dihydropyrimidinase (DPYS)
ORF Plasmid: tGFP
"NM_001385" in other vectors (4)
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CNY 3,864.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DHP; DHPase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206603 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCCCTCGCGGCTCCTGATCCGCGGGGGTCGCGTGGTCAACGATGACTTCTCGGAGGTGGCCG ACGTGCTGGTGGAGGACGGCGTGGTGCGGGCACTCGGGCACGACCTGCTGCCTCCCGGGGGCGCTCCTGC GGGGCTGCGGGTCCTCGACGCCGCCGGCAAGCTCGTCCTGCCCGGAGGCATCGACACACACACGCACATG CAGTTCCCCTTCATGGGCTCGCGGTCCATCGACGACTTCCACCAGGGCACCAAGGCTGCTCTCTCAGGAG GCACCACCATGATTATTGATTTCGCCATTCCTCAGAAAGGTGGCTCCCTCATTGAGGCCTTCGAGACCTG GCGAAGCTGGGCTGATCCCAAAGTTTGCTGCGACTACAGCCTTCATGTGGCAGTGACGTGGTGGAGTGAC CAGGTTAAAGAAGAAATGAAAATCCTTGTGCAAGATAAAGGTGTTAACTCTTTCAAGATGTTTATGGCCT ATAAAGATCTGTACATGGTGACAGACCTGGAGCTGTACGAAGCCTTCTCTCGGTGCAAGGAAATTGGAGC AATTGCCCAGGTCCATGCGGAAAATGGAGACTTAATTGCAGAGGGAGCAAAGAAGATGTTGGCTCTGGGG ATAACAGGCCCTGAGGGCCACGAGCTGTGCCGCCCAGAGGCAGTGGAGGCAGAGGCCACGCTGAGAGCCA TCACCATAGCCAGCGCTGTGAACTGTCCTCTCTACATTGTGCATGTGATGAGCAAGTCTGCAGCTAAGGT GATAGCGGATGCAAGGAGAGATGGGAAGGTGGTCTATGGTGAACCCATAGCAGCCAGTCTTGGCACAGAT GGCACTCACTACTGGAATAAAGAATGGCACCATGCAGCCCACCATGTCATGGGTCCACCTTTGCGACCAG ACCCCTCAACACCCGACTTCCTCATGAATCTGTTGGCTAATGATGATCTAACCACAACAGGGACTGATAA CTGCACTTTCAACACCTGCCAGAAAGCTCTTGGGAAGGATGATTTTACCAAGATCCCCAATGGGGTGAAT GGTGTTGAAGATCGGATGTCCGTAATATGGGAAAAAGGCGTGCATAGTGGTAAAATGGATGAAAACAGAT TTGTGGCAGTTACCAGCACAAATGCAGCCAAAATTTTTAATCTCTATCCAAGAAAAGGAAGAATAGCTGT AGGATCAGATGCTGACATTGTTATTTGGGACCCAAAAGGCACAAGGACTATCTCAGCAAAAACTCATCAT CAGGCTGTTAACTTCAACATTTTCGAGGGCATGGTTTGCCACGGGGTGCCCCTTGTGACTATTTCAAGAG GCAAAGTGGTATATGAAGCCGGAGTGTTCAGTGTCACGGCAGGAGATGGGAAGTTTATTCCTCGAAAACC ATTTGCTGAATATATTTACAAACGAATAAAGCAGCGAGACCGGACTTGCACACCTACCCCTGTGGAGCGT GCACCCTATAAGGGAGAAGTCGCCACACTGAAATCCAGAGTGACAAAAGAAGATGCCACAGCAGGGACCA GGAAACAGGCCCACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206603 protein sequence
Red=Cloning site Green=Tags(s) MAAPSRLLIRGGRVVNDDFSEVADVLVEDGVVRALGHDLLPPGGAPAGLRVLDAAGKLVLPGGIDTHTHM QFPFMGSRSIDDFHQGTKAALSGGTTMIIDFAIPQKGGSLIEAFETWRSWADPKVCCDYSLHVAVTWWSD QVKEEMKILVQDKGVNSFKMFMAYKDLYMVTDLELYEAFSRCKEIGAIAQVHAENGDLIAEGAKKMLALG ITGPEGHELCRPEAVEAEATLRAITIASAVNCPLYIVHVMSKSAAKVIADARRDGKVVYGEPIAASLGTD GTHYWNKEWHHAAHHVMGPPLRPDPSTPDFLMNLLANDDLTTTGTDNCTFNTCQKALGKDDFTKIPNGVN GVEDRMSVIWEKGVHSGKMDENRFVAVTSTNAAKIFNLYPRKGRIAVGSDADIVIWDPKGTRTISAKTHH QAVNFNIFEGMVCHGVPLVTISRGKVVYEAGVFSVTAGDGKFIPRKPFAEYIYKRIKQRDRTCTPTPVER APYKGEVATLKSRVTKEDATAGTRKQAHP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001385 |
| ORF Size | 1557 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001385.3 |
| RefSeq Size | 2197 bp |
| RefSeq ORF | 1560 bp |
| Locus ID | 1807 |
| UniProt ID | Q14117 |
| Domains | Amidohydro_1 |
| Protein Families | Druggable Genome |
| Protein Pathways | beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism |
| MW | 56.6 kDa |
| Gene Summary | Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206603L3 | Lenti ORF clone of Human dihydropyrimidinase (DPYS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206603L4 | Lenti ORF clone of Human dihydropyrimidinase (DPYS), mGFP tagged |
CNY 5,890.00 |
|
| RG206603 | DPYS (tGFP-tagged) - Human dihydropyrimidinase (DPYS) |
CNY 5,464.00 |
|
| SC321808 | DPYS (untagged)-Human dihydropyrimidinase (DPYS) |
CNY 3,872.00 |
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