NMNAT3 (NM_178177) Human Tagged ORF Clone
CAT#: RC206772
NMNAT3 (Myc-DDK-tagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1
ORF Plasmid: tGFP
"NM_178177" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FKSG76; PNAT-3; PNAT3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCAGGTCATCCAGGGTATCATCTCTCCTGTCAACGACACCTATGGGAAGAAAGACCTCGCAGCTT CTCATCACCGAGTGGCCATGGCCCGGCTGGCCCTGCAGACATCCGACTGGATCCGGGTGGACCCTTGGGA GAGTGAGCAGGCACAGTGGATGGAGACAGTGAAGGTGCTGAGGCATCATCACAGCAAACTGCTCAGATCT CCACCCCAGATGGAAGGCCCAGACCATGGCAAGGCACTCTTCTCGACCCCTGCAGCTGTGCCTGAGCTGA AGCTTCTCTGTGGGGCAGACGTCTTGAAGACCTTCCAGACCCCCAACCTCTGGAAGGATGCGCACATCCA GGAAATAGTGGAGAAGTTTGGCTTGGTGTGCGTGGGCCGAGTAGGTCACGACCCAAAAGGTTACATCGCA GAATCTCCCATCCTACGGATGCACCAGCACAACATTCACCTGGCCAAGGAGCCTGTGCAGAATGAGATCA GTGCCACATACATCAGGCGAGCCTTGGGCCAAGGGCAGAGCGTAAAGTACCTGATTCCCGATGCTGTCAT CACGTACATCAAGGACCATGGCCTCTACACCAAGGGCAGTACCTGGAAAGGCAAAAGCACCCAGAGCACT GAGGGCAAGACAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206772 protein sequence
Red=Cloning site Green=Tags(s) MYQVIQGIISPVNDTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHHHSKLLRS PPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKDAHIQEIVEKFGLVCVGRVGHDPKGYIA ESPILRMHQHNIHLAKEPVQNEISATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQST EGKTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_178177 |
ORF Size | 645 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178177.4 |
RefSeq Size | 1919 bp |
RefSeq ORF | 648 bp |
Locus ID | 349565 |
UniProt ID | Q96T66 |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
MW | 24.1 kDa |
Gene Summary | This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification
,Yagi, M;Toshima, T;Amamoto, R;Do, Y;Hirai, H;Setoyama, D;Kang, D;Uchiumi, T;,
The EMBO journal
,PubMed ID 33528041
[NMNAT3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206772L1 | Lenti-ORF clone of NMNAT3 (Myc-DDK-tagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 6,000.00 |
|
RC206772L2 | Lenti-ORF clone of NMNAT3 (mGFP-tagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 5,890.00 |
|
RC206772L3 | Lenti-ORF clone of NMNAT3 (Myc-DDK-tagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 5,890.00 |
|
RC206772L4 | Lenti-ORF clone of NMNAT3 (mGFP-tagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 5,890.00 |
|
RG206772 | NMNAT3 (tGFP-tagged) - Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 5,200.00 |
|
SC127956 | NMNAT3 (untagged)-Human nicotinamide nucleotide adenylyltransferase 3 (NMNAT3), transcript variant 1 |
CNY 2,400.00 |