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RGS5 (NM_003617) Human Tagged ORF Clone
CAT＃: RC206857
RGS5 (Myc-DDK-tagged)-Human regulator of G-protein signaling 5 (RGS5), transcript variant 1
ORF Plasmid: tGFP
"NM_003617" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCAAAGGACTTGCAGCTTTGCCCCACTCATGCCTGGAAAGGGCCAAGGAGATTAAGATCAAGTTGG GAATTCTCCTCCAGAAGCCAGACTCAGTTGGTGACCTTGTCATTCCGTACAATGAGAAGCCAGAGAAACC AGCCAAGACCCAGAAAACCTCGCTGGACGAGGCCCTGCAGTGGCGTGATTCCCTGGACAAACTCCTGCAG AACAACTATGGACTTGCCAGTTTCAAAAGTTTCCTGAAGTCTGAATTCAGTGAGGAAAACCTTGAGTTCT GGATTGCCTGTGAGGATTACAAGAAGATCAAGTCCCCTGCCAAGATGGCTGAGAAGGCAAAGCAAATTTA TGAAGAATTCATTCAAACGGAGGCTCCTAAAGAGGTGAATATTGACCACTTCACTAAGGACATCACAATG AAGAACCTGGTGGAACCTTCCCTGAGCAGCTTTGACATGGCCCAGAAAAGAATCCATGCCCTGATGGAAA AGGATTCTCTGCCTCGCTTTGTGCGCTCTGAGTTTTATCAGGAGTTAATCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206857 protein sequence
Red=Cloning site Green=Tags(s) MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQ NNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITM KNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003617 |
| ORF Size | 543 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003617.4 |
| RefSeq Size | 5927 bp |
| RefSeq ORF | 546 bp |
| Locus ID | 8490 |
| UniProt ID | O15539 |
| Domains | RGS |
| Protein Families | Druggable Genome |
| MW | 20.9 kDa |
| Gene Summary | This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206857L1 | Lenti ORF clone of Human regulator of G-protein signaling 5 (RGS5), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206857L2 | Lenti ORF clone of Human regulator of G-protein signaling 5 (RGS5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206857L3 | Lenti ORF clone of Human regulator of G-protein signaling 5 (RGS5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206857L4 | Lenti ORF clone of Human regulator of G-protein signaling 5 (RGS5), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG206857 | RGS5 (tGFP-tagged) - Human regulator of G-protein signaling 5 (RGS5), transcript variant 1 |
CNY 4,000.00 |
|
| SC117891 | RGS5 (untagged)-Human regulator of G-protein signaling 5 (RGS5), transcript variant 1 |
CNY 2,400.00 |
